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Summary

A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min−1. The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL−1 with the limit of detection of 3.5 ng mL−1 (S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.

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Summary

A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid-liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and C max values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.

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(‘music’), while lágy (‘soft’) and puha (‘tender’) are synonyms in the context of margarin (‘margarine’) and fém (‘metal’). Our main objective in the present proof-of concept experiment is the detection of adjectival cliques of this type: cliques

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satellites as reference points for geodesic surveys and computers to speed up the recording and processing of survey data. The use of the Global Positioning System (GPS). as well as of Light Detection and Ranging (LiDAR) 3D laser scanners have aided in

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dataset will be extended with the corpus occurrences of complex forms listed above. However, in this initial phase, even the accurate detection of simple forms poses a great challenge which is mainly due to their rather free distribution relative to the

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Broadcast speech transcription and spoken term detection . 2nd IEEE International Conference on Cognitive Infocommunications (CogInfoCom 2011) . Tarján , Balázs , Gellért Sárosi , Tibor Fegyó and Péter Mihajlik . 2013 . Improved recognition of

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selection for rapid detection of snakes . Proceedings of the National Academy of Sciences 110 ( 47 ). 19000 – 19005 . 10.1073/pnas.1312648110 Maudslay , Rowan Hall and Ryan Cotterell . 2021 . Do syntactic probes probe syntax? Experiments with

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variables, as introduced by Strobl and her colleagues. 50 This measure is conditional in the sense that, when calculating variable importance for a given variable other variables are taken into account, and therefore this method can enable the detection of

Open access

to a depth of several tens of micrometers. With the standard PIXE setup, elements from Al to U can be detected simultaneously, and with appropriate measurement configurations, sensitivity down to ppm(µg g −1 ) detection limit can be achieved. 3 The

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Follow the Rivers?•

Group identity, interactions and the role of rivers in late Hallstatt southern Pannonia

Folyók mentén?•

Csoportidentitás, interakciók és a folyók szerepe a késő Hallstatt-kori dél-pannoniai régióban
Archaeologiai Értesítő
Author:
Bence Soós

Series 1378 . Oxford , 45 – 52 . Mazzucato , Camilla 2019 Socio-Material Archaeological Networks at Çatalhöyük a Community Detection Approach . Frontiers in Digital Humanities 6 : 8 . DOI: https://doi.org/10.3389/fdigh.2019.00008 Márton

Open access