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, Jung M , Meller S , Kristiansen G Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition . PLoS One 8 , 1 – 10 ( 2013 ) 3

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, Thomson RB Jr. , Peterson LR , Kaul KL : Real-time PCR for detection and identification of Plasmodium spp . J Clin Microbiol 43 , 2435 – 2440 ( 2005 ) 8. Berry

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the spread of MRSA and SA [ 6 , 7 ]. The German MRSA screening guidelines [ 8 ] recommend culture-based methods as the basis of MRSA screening. Due to the high negative predictive value (NPV) and rapid turnaround time, PCR-based methods are

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biopsies. If only cysts are seen, discrimination of E. histolytica from non-pathogenic species can be performed either by PCR or antigen testing [ 2 ]. While diagnostic accuracy of modern real-time PCR for the detection of E. histolytica in stool

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European Journal of Microbiology and Immunology
Authors:
Alexis Lacout
,
Marie Mas
,
Julie Pajaud
,
Véronique Perronne
,
Yannick Lequette
,
Michel Franck
, and
Christian Perronne

patients suffering from polymorphic signs and symptoms (SPPT/PTLDS), using real time qPCR, which is a direct diagnostic method amplifying the DNA of the microorganisms sought. As the yield of PCRs looking for Borrelia or co-infections in the venous blood is

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. Pierce VM , Elkan M , Leet M , McGowan KL , Hodinka RL : Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children . J Clin Microbiol 50 , 364 – 371 ( 2012

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1990 28 1982 1987 Loeffelholz MJ, Thompson C, Long KS, Gilchrist MJR: Comparison of PCR, culture and direct fluorescent

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Acta Alimentaria
Authors:
J. Krulj
,
N. Ćurčıć
,
A. Bočarov Stančıć
,
J. Kojıć
,
L. Pezo
,
L. Peıć Tukuljac
, and
M. Bodroža Solarov

spp. based on morphological attributes is not unusual ( Wanu et al., 2001 ). Therefore, most identification methodologies are now based on DNA detection by using polymerase chain reaction (PCR) methods. The ITS region is considered to be a universal and

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) for the detection of C. difficile GDH and toxins A/B in stool samples were used according to the manufacturer's instructions. Polymerase Chain Reaction (PCR) Assay Samples with discordant EIA results were tested by

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A SARS-CoV-2-járvány kihívásai és tapasztalatai a molekuláris diagnosztikában

Challenges and experiences of the SARS-CoV-2 pandemic in molecular diagnostics

Orvosi Hetilap
Authors:
András Zóka
,
Bálint Tresó
, and
Gabriella Bekő

References 1 Tresó B. Real-time PCR. In: Takács M. (ed.) Clinical and epidemiological virology. [Real-time PCR. In: Takács M. (szerk.) Klinikai és

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