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A SARS-CoV-2-járvány kihívásai és tapasztalatai a molekuláris diagnosztikában

Challenges and experiences of the SARS-CoV-2 pandemic in molecular diagnostics

Orvosi Hetilap
Authors:
András Zóka
,
Bálint Tresó
, and
Gabriella Bekő

References 1 Tresó B. Real-time PCR. In: Takács M. (ed.) Clinical and epidemiological virology. [Real-time PCR. In: Takács M. (szerk.) Klinikai és

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Acta Veterinaria Hungarica
Authors:
Zsolt Becker
,
Noémi Holló
,
Róbert Farkas
,
Mónika Gyurkovszky
,
Jenő Reiczigel
,
Krisztián Olaszy
,
Zoltán Vári
, and
Károly Vörös

applicable for diagnosing microfilaraemia without definitive distinction of D. immitis and D. repens ( Magnis et al., 2013 ). The polymerase chain reaction (PCR) technique as a molecular biological method can be considered the most sensitive

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Zoonoses Report ( European Food Safety Authority, 2022 ). PCR-based molecular assays are already available for the detection of Salmonella infection ( Aabo et al., 1993 ; Szmolka et al., 2006 ). However, these assays were developed for the detection of

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Physiology International
Authors:
András Végh
,
Anita Csorba
,
Ákos Koller
,
Behnam Mohammadpour
,
Petra Killik
,
Lilla István
,
Márton Magyar
,
Teréz Fenesi
, and
Zoltán Zsolt Nagy

between January and April of 2021. During recovery, both nasopharyngeal and conjunctival swabs were taken and analyzed with real-time polymerase chain reaction (PCR) in order to show the presence of SARS-CoV-2 RNA in the sample (in the Central Laboratory

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, no other obvious signs of disease or mortality were observed among the 2-year-old wels catfish. One fish with the most prominent rash was used for PCR testing for a possible viral origin of the skin disease. A pea-sized piece from the damaged skin

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Acta Microbiologica et Immunologica Hungarica
Authors:
Zaira Moure
,
Elena Cuadros
,
Daniel Pablo-Marcos
,
María José Reina
,
Inés de Benito
, and
Ana Belén Campo

on real-time RT-PCR (RT-qPCR), are the main tools for the detection of SARS-CoV-2, the diagnosis was initially centralized at reference hospitals, with well-equipped microbiology departments and qualified staff [ 2 , 3 ]. Within a few weeks, new

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To explore the diversity of some DNA viruses in reptiles, a continuous screening is going on, in our laboratory, by PCR using different consensus primers designed for the detection of the most conserved genome regions of adeno-, herpes- and parvoviruses. The test material consists essentially of dead specimens collected randomly from private pet owners, local pet shops, or at occasional exotic pet fairs. Here we report the partial sequence of a putative novel parvovirus obtained from a dead checkerboard worm lizard (Trogonophis wiegmanni) that had been wild-caught in its native habitat. An in-house-developed PCR with consensus primers targeting the gene of the parvoviral capsid protein was used. Other PCRs, intended to detect certain large DNA viruses, remained negative. The sequence of the PCR product indicated the presence of a hitherto unknown parvovirus in the internal organs of the checkerboard worm lizard. In phylogeny reconstruction, the novel sequence clustered with the members of the Dependovirus genus of the Parvoririnae subfamily, closest to the branch of snake adeno-associated virus. Since we could not demonstrate the presence of a potential helper virus, the putative amphisbaenian parvovirus supposedly can replicate autonomously. This is the first virus infection ever detected in any members of the suborder Amphisbaenia, and only the third parvoviral sequence obtained from any reptilian host.

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Tartós SARS-CoV-2-PCR-pozitivitáshoz társuló, lokoregionálisan előrehaladott emlődaganat komplex onkológiai kezelése

Complex oncologic therapy for loco-regionally advanced breast cancer associated with long-lasting SARS-CoV-2 PCR-positivity

Orvosi Hetilap
Authors:
András Drozgyik
,
Dániel Kollár
,
Márta Knausz
,
István Sipőcz
,
F. Tamás Molnár
, and
Tamás Kullmann

Összefoglaló. A COVID–19 mortalitását a súlyos társbetegségek, közöttük bizonyos daganatos betegségek is növelik. Immunszuppresszív hatásuk miatt felmerülhet a citotoxikus kezelések rizikónövelő hatása is. Ugyanakkor az onkológiai terápia megszakítása vagy halasztása, különösen az agresszívebb, kiterjedtebb és fiatalkorban jelentkező daganatok esetében ronthatja a kórjóslatot. Egy 39 éves nőbeteg esetét ismertetjük. A járvány során késlekedve felismert, lokoregionálisan kiterjedt emlődaganat miatt primer szisztémás kemoterápiában részesült. A kezelés 5. ciklusa során enyhe légúti tünetek kapcsán, az onkológiai ambulancián SARS-CoV-2-fertőzése igazolódott. Kemoterápiás kezelését felfüggesztettük. A diagnózistól számított 3. napon tünetmentessé vált, ám SARS-CoV-2-PCR-pozitivitása még a 43. napon is fennállt. A 19. napon hormongátló kezelést indítottunk. Az 51. napon mastectomia és axillaris block dissectio történt. A 82. napon a megszakított kemoterápiát a hormongátló kezelés leállítását követően G-CSF-profilaxis mellett újraindítottuk. A kezelés során fertőzéses szövődményt nem észleltünk. Kemoterápia és műtét SARS-CoV-2-fertőzött, tünetmentes daganatos betegnél szövődménymentesen végezhető elhúzódó virológiai pozitivitás esetén, felszabadító vizsgálat nélkül is. A daganatos betegek koronavírus-fertőzése esetén az onkológiai protokolltól történő eltérés egyénre szabott optimalizálásával és a multidiszciplináris team szorosabb együttműködésével az infektológiai és az onkológiai kockázat együttes alacsonyan tartása is megvalósítható. Orv Hetil. 2021; 162(16): 611–614.

Summary. Mortality of COVID-19 is increased when certain co-morbidities, among others advanced malignancies are present. Deleterious effect of cytotoxic therapy, related to its immunosuppressive effect, may also be hypothesised. However, postponing or cancelling oncologic treatment, especially in younger patients with advanced and more aggressive tumors may worsen the prognosis. The case of a 39-year-old female patient is presented, who was diagnosed with loco-regionally advanced breast cancer during the pandemic. Primary systemic chemotherapy was started. The patient presented with acute respiratory tract symptoms during the fifth cycle and subsequently SARS-CoV-2 infection was diagnosed. Chemotherapy was cancelled. Symptoms resolved in three days after diagnosis. SARS-CoV-2 PCR remained positive up to day 43. Antihormonal therapy was introduced on day 19 and she underwent mastectomy with axillary lymph node dissection on day 51. Chemotherapy was reset postoperatively on day 82 with prophylactic G-CSF protection. No adverse event was observed throughout the treatment. Cytotoxic chemotherapy and surgery can be successfully delivered in breast cancer patients with prolonged asymptomatic SARS-CoV-2 PCR positivity, even without negative swab result. Individual optimisation of the therapy may require deviations from standard protocols. Closer multidisciplinary cooperation may contribute to the minimisation of both oncologic and infectious risks. Orv Hetil. 2021; 162(16): 611–614.

Open access
Acta Veterinaria Hungarica
Authors:
Sándor Szekeres
,
Alexandra Juhász
,
Milán Kondor
,
Nóra Takács
,
László Sugár
, and
Sándor Hornok

Reports of Sarcocystis rileyi-like protozoa (‘rice breast disease’) from anseriform birds had been rare in Europe until the last two decades, when S. rileyi was identified in northern Europe and the UK. However, despite the economic losses resulting from S. rileyi infection, no recent accounts are available on its presence (which can be suspected) in most parts of central, western, southern and eastern Europe. Between 2014 and 2019, twelve mallards (Anas platyrhynchos) were observed to have rice breast disease in Hungary, and the last one of these 12 cases allowed molecular identification of S. rileyi, as reported here. In addition, S. rileyi was molecularly identified in the faeces of one red fox (Vulpes vulpes). The hunting season for mallards in Hungary lasts from mid-August to January, which in Europe coincides with the wintering migration of anseriform birds towards the south. Based on this, as well as bird ringing data, it is reasonable to suppose that the first S. rileyi-infected mallards arrived in Hungary from the north. on the other hand, red foxes (Vulpes vulpes), which are final hosts of S. rileyi, are ubiquitous in Hungary, and our molecular finding confirms an already established autochthonous life cycle of S. rileyi in the region. Taken together, this is the first evidence for the occurrence of S. rileyi in Hungary and its region.

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Alsó légúti minták molekuláris mikrobiológiai vizsgálata a koronavírus-járvány időszakában

Molecular microbiological testing of lower respiratory tract samples during COVID–19 pandemic

Orvosi Hetilap
Authors:
Sándor Károlyi
,
Emese Juhász
,
Miklós Iván
,
Edina Szabó
,
Petronella Farkas
,
Kamilla Székely
, and
Katalin Kristóf

: e00024-17. 2 Lee SH, Ruan SY, Pan SC, et al. Performance of a multiplex PCR pneumonia panel for the identification of respiratory pathogens and the main

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