A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous analysis of three triterpene acids (corosolic, oleanolic, and ursolic acids) in extracts from inflorescences, leaves, and fruits of Prunus serotina Ehrh. (American black cherry). Separation of the acids was accomplished on a C18 column (5 μm, 250 mm × 4.6 mm i.d.) and recorded at 210 nm. The greatest resolution was achieved with 90:10 (υ/υ) methanol-1% aqueous orthophosphoric acid as mobile phase at a flow rate of 0.6 mL min−1. The correlation coefficients for all the calibration plots (r > 0.998) showed linearity good over the range tested. The relative standard deviation of the method was less than 3.3% for intra and inter-day assays, and average recovery was between 95.9 and 100.9%. Sensitivity was high; detection limits were between 0.034 and 0.067 μg mL−1. Total amounts of triterpene acids were 0.451–0.928, 0.031, and 0.911–1.455% in the inflorescences, fruits, and leaves of P. serotina, depending on the time of harvesting.
Authors:A. Wesołowska, D. Jadczak, and M. Grzeszczuk
The essential oil of the herb Nepeta cataria L. var. citriodora was obtained by hydrodistillation in Dean-Stark apparatus and by steam distillation. The chemical composition of the oil was determined by GC-MS. Two different GC oven temperature programs were tested. The main constituents of all the samples studied were the monoterpene alcohols (R)-(+)-β-citronellol and geraniol. The largest amounts of monoterpene aldehydes (geranial and neral) were found in the hydrodistilled oil. α-Amyrin (a precursor of ursolic acid) was also detected in the oil. trans-Rose oxide, α-terpineol, geranic acid, and trace amount of rose-oxide were found in the steam distilled oil only.
Authors:A. Jóźwiak, G. Jóźwiak, and M. Waksmundzka-Hajnos
A reversed-phase high-performance liquid chromatography diode array detector (HPLC-DAD) method was developed for the separation of triterpene acids and their determination in crude extracts of Potentilla species. Symmetry octadecylsilyl silica (ODS) column with acetonitrile and water with phosphoric acid (pH = 2.5) as the mobile phase was used in the described HPLC system. The column temperature was at 20 °C, the flow rate was 0.8 mL/min. The detection wavelength was 210 nm. For quantitative purposes, the linear dependencies of analyte concentration and peak surface area were obtained with regression coefficient values 0.9983 < r2 < 0.9995. For euscaphic acid, calibration was performed in the range of 0.07–0.525; 0.7–2.1 mg/mL, for pomolic acid in the range of 0.019–0.171; 0.209–0.665 mg/mL, for ursolic acid in the range of 0.2–1.375–3.3 mg/mL. The observed recovery of triterpene acids was from 95.3% to 103.1%. The observed values of RSD were well within the generally acceptable limit of no more than 5%. This method was successfully applied to quantify pomolic acid (PA), ursolic acid (UA) and the sum of euscaphic (EA) and tormentic acids (TA) (expressed as euscaphic acid) in roots and rhizomes of 15 various Potentilla species and 2 subspecies (i.e., P. atrosanguinea, P. recta).