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Acta Alimentaria
Authors: Á. Tóth, E. Baka, Sz. Luzics, I. Bata-Vidács, I. Nagy, B. Bálint, R. Herczeg, F. Olasz, T. Wilk, T. Nagy, B. Kriszt, I. Nagy, and J. Kukolya

Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

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). The functionality of rye AXs can be investigated by enzymatic treatments. It has been shown that the encapsulation of proteins by AX can be reduced by the addition of xylanase ( Grossmann et al., 2016 ; Döring et al., 2017 ). The treatment of rye

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agitation rates to improve cellulase-free xylanase production by thermotolerant Streptomyces sp. Ab 106 and repeated fed-batch cultivation using agricultural waste . J. Biosci. Bioeng. , 95 ( 3 ), 298 - 301 . 10.1016/S1389-1723(03)80033-6 Yen , H

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