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The negative samples of a collection, established originally for seeking new adeno- and herpesviruses in lower vertebrates, were screened for the pres-ence of circoviruses by a consensus nested PCR targeting the gene coding for the replication-associated protein. Six fish samples representing five species, namely asp (Aspius aspius), roach (Rutilus rutilus), common bream (Abramis brama), round goby (Neogobius melanostomus) and monkey goby (Neogobius fluviatilis), as well as three frog samples were found positive for circoviral DNA. Sequence analysis of the amplicons indicated the presence of three novel putative circo-like viruses and a circovirus in Hungarian fishes and one novel circovirus in a common toad (Bufo bufo), and another one in a dead and an alive specimen of green tree frog (Litoria caerulea), respectively. In phylogeny reconstruction, the putative bream circovirus clustered together with circoviruses discovered in other cyprinid fishes recently. Three other piscine circoviral sequences appeared closest to sequences derived from different environmental samples. Surprisingly, the nucleotide sequence derived from two fish samples (a bream and a monkey goby) proved to be from porcine circovirus 2 (PCV2), almost identical to a sequence detected in Sweden previously. This is the first report on the detection of PCV2 in fish and circoviral DNA in amphibian hosts.

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Acta Veterinaria Hungarica
Authors:
Gábor Cech
,
Diána Sándor
,
Kálmán Molnár
,
Petra Paulus
,
Melitta Papp
,
Bálint Preiszner
,
Zoltán Vitál
,
Ádám Varga
, and
Csaba Székely

from the River Danube at Szentendre and a single specimen from the River Ipoly at Ipolytölgyes was examined ( Table 2 ). Besides 142 pumpkinseeds, 16 specimens of roach ( Rutilus rutilus ) and 11 white breams ( Blicca bjoerkna ) were collected from Lake

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the uniformity value 0.3, which is required by the BREEAM standard. Fig. 6. Daylight illuminance comparison between bream standard and the living room and Room 1 a) illuminance in the summer (June); b) illuminance in the winter (December) Fig. 7

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. Infect Immun 61 ( 5 ), 1601 – 1610 ( 1993 ) 3. El-Omar EM , Carrington M , Chow WH , McColl KE , Bream JH , Young HA Herrera J , Lissowska

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the causative agent of the so-called carp pox disease in common carp ( Sano et al., 1985 ). Similar clinical signs were reported in other fish species of the family Cyprinidae, including barbel, bleak ( Alburnus alburnus ), freshwater bream ( Abramis

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higher production and consumption in Tunisia, the studied species of fish was Bream ( Sparus auratus ). Fish samples were collected from different aquaculture firms in Tunisia, immediately frozen below −20 °C with the aim of avoiding a possible antibiotic

Open access
Acta Alimentaria
Authors:
Sz. Luzics
,
Á. Tóth
,
T. Barna
,
E. Szabó
,
I. Nagy
,
B. Horváth
,
I. Nagy
,
Z. Varecza
,
I. Bata-Vidács
, and
J. Kukolya

, feed utilisation, intestinal histology and gut microbiota of gilthead sea bream ( Sparus aurata ) . Aquaculture , 300 ( 1–4 ): 182 – 188 . https://doi.org/10.1016/j.aquaculture.2010.01.015 . Eriksson , K.E.L. , Blanchette R.H. , and Ander , P

Open access