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The present work aimed to develop and validate a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of metformin hydrochloride and vildagliptin in tablet and biological samples. Isocratic elution of both the analytes was performed at 35 °C by injecting 20 μL into Thermo Hypersil ODS C18 column (5 μm, 4.6 mm× 250 mm), while the flow rate was set to 0.8 mL/min. The mobile phase comprised of methanol, acetonitrile, and phosphate buffer (5:30:65, v/v, pH 3.5), and wavelength was selected at 212 nm. The overall run time per sample was 7.0 min with a retention time of 3.36 and 5.41 min for metformin hydrochloride and vildagliptin, respectively. The calibration curve was linear from 10–140 μg/mL for metformin and 1–14 μg/mL for vildagliptin with a coefficient of determination (R 2) ≤ 0.9919, while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.13 and 0.97%, respectively. Force degradation studies indicated a complete separation of the analytes in the presence of their degradation products providing a high degree of method specificity. The proposed reversed-phase high-performance liquid chromatography (RP-HPLC) method was demonstrated to be simple and rapid for the determination of metformin hydrochloride and vildagliptin in commercially available tablet and biological samples providing recoveries ranged between 100.13–100.29%.

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1. Introduction Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycaemia, in which these two are the most common diseases of this era, and statistically, over 300 million people are affected by

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Summary

Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L−1) + KN (0.5 mg L−1) under abiotic stress conditions at 45 days determined by growth curve analysis. GA detection and quantification were carried out using thin-layer chromatography (TLC), highperformance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA. Quantification of GA had showed the linearity, accuracy, robustness and precision by HPLC. GA content was significantly higher in gravimetric method than HPLC. All these methods were found to be simple, accurate, selective and rapid and could be successfully applied for the determination of GA. It could have potential as a pharmaceutical drug for Type 1 diabetes mellitus (IDDM) and obesity.

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Summary

Artemisia pallens L. (Compositae) is used in Indian traditional medicine to treat diabetes mellitus, jaundice, hysteria, body pain, and bacterial and fungal infections. A major cause of a variety of diseases is oxidative stress which is reduced by antioxidants such as polyphenols. These secondary metabolites are generally ubiquitous in plants and extensively used in the pharmaceutical, cosmetic, and food industries. In this study a simple and sensitive HPLC-UV-MS-MS-based method was developed for separation, identification, and quantification of polyphenols, for example gallic, protocatechuic, chlorogenic, caffeic, and ferulic acids, rutin, quercetin, and kaempferol. Amounts of polyphenols detected in 50% methanol-water extracts of the plant varied from 0.005% (kaempferol) to 0.24% (protocatechuic acid). Separation of the polyphenols was achieved on a reversed-phase C18 with a mobile phase prepared from 1% aqueous with acetic acid and acetonitrile at a flow rate of 0.6 mL min−1. The phenolic compounds were detected by UV absorption at 254 nm. The method was validated for linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and compound stability. Results from intra and inter-day validation (n = 6) showed the method was efficient and rapid. The optimized method was applied to extracts of A. pallens for identification and quantification of the polyphenols. The reference standards and their presence in A. pallens were confirmed by mass spectrometry.

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1. Introduction Oral antidiabetic drugs are widely used for treatment of diabetes mellitus type II. It is the most common diabetes disease caused by fatty acids and myocells resistance to insulin. Its treatments include

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Introduction In recent years, the advantages of Herbal and Traditional Chinese Medicine in the prevention and treatment of diabetes mellitus (DM) have been widely recognized around the world [ 1 ,  2 ]. Bai-Hu-Jia-Ren-Shen-Tang Decoction (BHJRSTD

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Introduction Diabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia which on progression leads to many secondary complications [ 1 ]. The function of vascular endothelial cells in

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, diabetes mellitus , bleeding, osteoporosis, infections, muscle weakness and delayed healing of wounds. Seriousness of adverse effects depends on corticosteroids’ potency, skin area where the drug is applied and the duration of treatment. If the formulation

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easily soluble in methanol, acetonitrile, and chloroform [ 6 ]. ALA is extensively used in food supplements due to its antioxidant properties and finds wide clinical applicability in the management of many ailments such as diabetes mellitus, hypertension

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-insulin-dependent type II diabetes mellitus [ 1 , 2 ]. Many generic products have been produced and distributed in Egypt and many other countries. We need to ensure that all these generic products have similar quality and efficacy as the brand product. So, there is

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