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adhesion to fibronectin (CadF) [ 23 , 24 ], major outer membrane protein (MOMP) [ 25 ], periplasmic binding protein (PEB1) [ 26 ], P95 [ 27 ], jejuni lipoprotein A (JlpA) [ 28 , 29 ], Campylobacter autotransporter protein A (CapA) [ 30 ], and
Using quantitative phosphopeptide sequencing of unstimulated versus stimulated primary murine Foxp3+ regulatory and Foxp3− conventional T cells (Tregs and Tconv, respectively), we detected a novel and differentially regulated tyrosine phosphorylation site within the C1 domain of the guanine-nucleotide exchange factor CalDAG GEFI. We hypothesized that the Treg-specific and activation-dependent reduced phosphorylation at Y523 allows binding of CalDAG GEFI to diacylglycerol, thereby impacting the formation of a Treg-specific immunological synapse. However, diacylglycerol binding assays of phosphomutant C1 domains of CalDAG GEFI could not confirm this hypothesis. Moreover, CalDAG GEFI−/− mice displayed normal Treg numbers in thymus and secondary lymphoid organs, and CalDAG GEFI−/− Tregs showed unaltered in vitro suppressive capacity when compared to CalDAG GEFI+/+ Tregs. Interestingly, when tested in vivo, CalDAG GEFI−/− Tregs displayed a slightly reduced suppressive ability in the transfer colitis model when compared to CalDAG GEFI+/+ Tregs. Additionally, CRISPR-Cas9-generated CalDAG GEFI−/− Jurkat T cell clones showed reduced adhesion to ICAM-1 and fibronectin when compared to CalDAG GEFI-competent Jurkat T cells. Therefore, we speculate that deficiency in CalDAG GEFI impairs adherence of Tregs to antigen-presenting cells, thereby impeding formation of a fully functional immunological synapse, which finally results in a reduced suppressive potential.
931 Euker TP, Konkel ME: The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating
. 7 Reginald K, Westritschnig K, Linhart B, et al. Staphylococcus aureus fibronectin-binding protein specifically binds IgE from patients with atopic dermatitis and requires antigen
Mesterséges bőrszövetek a kutatásban és a gyógyításban
Tissue engineered skin products in research and therapeutic applications
template. Acta Biomater. 2017; 52: 33–40. 15 Schwarzbauer JE, DeSimone DW. Fibronectins, their fibrillogenesis, and in vivo functions. Cold Spring Harb Perspect Biol. 2011
Laboratories, Bratislava, Slovak Republic) were coated with 10 μg mL −1 fibronectin (Sigma-Aldrich, St. Louis, MO, USA), as optimal extracellular matrix support [ 14 ] overnight to model physiological circumstances. TR100 (kind gift from prof. Peter Gunning
atmosphere. The following day, potential growth inhibition zones around the discs were scrutinized by macroscopic inspection. For assessment of biofilm formation, experiments were performed in 96-well microtiter plates coated with human fibronectin
cells in microfluidic bioreactors for mAbs production [ 29 – 31 ]. They used different types of cell adhesion agents such as fibronectin [ 30 ] and poly- d -lysine [ 31 ] to improve the adhesion and viability. Green et al. tested the effect of different
, Calay Z , Aksu MF : Correlation between placental bed biopsy findings, vascular cell adhesion molecule and fibronectin levels in pre
Arencibia I , Suarez NC , Wolf-Watz H , Sundqvist KG . Yersinia invasin, a bacterial beta1-integrin ligand, is a potent inducer of lymphocyte motility and migration to collagen type IV and fibronectin . J Immunol . 1997