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Permeability glycoprotein (P-glycoprotein, Pgp) immunohistochemistry (IHC) was evaluated in dogs with multicentric lymphoma treated with cyclophosphamide– doxorubicin–vincristine–prednisolone with or without L-Asparaginase. Lymph nodes of 33 untreated dogs were immunophenotyped: Ki67% and Pgp analyses (with anti-Pgp, monoclonal mouse C494 clone) were performed. Pgp positivity rate and intensity were determined microscopically (by manual counting done by two blinded authors in two parallel specimens). The median overall survival time (OST) was 333 days and the relapse-free period (RFP) 134 days. Pgp expressions were positive in 18 out of 33 (54.5%) of tumour cells. T-cell types stained more intensively. Lower OST and RFP were found with Pgp positivity ≥ 35% (OST: 240 days, RFP: 95 days) compared to Pgp positivity < 35% (OST: 428 days, RFP: 232 days). Intensive staining was associated with a lower OST and RFP (240 and 103 days, respectively) than weak staining (428 and 221 days, respectively). Death due to adverse drug reactions was best predicted at Pgp positivity ≤ 6.5% (sensitivity/specificity: 0.55/0.81) and ≤ 123 days (sensitivity/ specificity: 0.55/0.86). Pgp evaluation by IHC can have prognostic value with a properly established Pgp% positivity cut-off value in dogs treated with Pgp substrate drugs.

Open access
European Journal of Microbiology and Immunology
Authors:
Cosme Alvarado-Esquivel
,
Luis Francisco Sánchez-Anguiano
,
Alejandra Mendoza-Larios
,
Jesús Hernández-Tinoco
,
José Francisco Pérez-Ochoa
,
Elizabeth Irasema Antuna-Salcido
,
Elizabeth Rábago-Sánchez
, and
Oliver Liesenfeld

The presence of tissue cysts of Toxoplasma gondii has only poorly been investigated in autopsy series. We determined the presence of T. gondii cysts in a series of 51 autopsies in a public hospital using immunohistochemistry of brain and heart tissues. The association of tissue cysts with the general characteristics of the autopsy cases was also investigated.

Of the 51 cases studied, five (9.8%) were positive by immunohistochemistry for T. gondii cysts in the brain. None of the heart specimens was positive for T. gondii cysts. The presence of T. gondii cysts in brains did not vary with age, sex, birthplace, residence, education, occupation, or the presence of pathology in the brain. In contrast, multivariate analysis showed that the presence of T. gondii cysts was associated with undernourishment (OR = 33.90; 95% CI: 2.82–406.32; P = 0.005).

We demonstrated cerebral T. gondii cysts in an autopsy series in Durango City, Mexico. Results suggest that T. gondii can be more readily found in brain than in heart of infected individuals. This is the first report of an association between the presence of T. gondii in brains and undernourishment.

Open access
European Journal of Microbiology and Immunology
Authors:
Laura Alejandra Mendoza-Larios
,
Fernando García-Dolores
,
Luis Francisco Sánchez-Anguiano
,
Elizabeth Irasema Antuna-Salcido
,
Jesús Hernández-Tinoco
,
Adriana Rocha-Salais
,
Marcela Araceli Segoviano-Mendoza
,
Antonio Sifuentes-Álvarez
, and
Cosme Alvarado-Esquivel

immunohistochemistry Brain tissues (amygdala and prefrontal cortex) of decedents were examined for detection of T. gondii using immunohistochemistry. Brain tissues were formalin-fixed, and paraffin-embedded sections were examined using the Tinto Detector Immuno DNA

Open access

„Dum spiro spero”: a SARS-CoV-2-fertőzés klinikopatológiája 26 eset kapcsán

“Dum spiro spero”: clinicopathologic characteristics of SARS-CoV-2 infection

Orvosi Hetilap
Authors:
Tamás Zombori
,
Levente Kuthi
,
Tibor Hortobágyi
,
Erika Csörgő
,
János Árgyelán
,
Lajos Kocsis
,
István Sejben
,
László Kaizer
,
Bence Radics
,
Anita Sejben
,
Tamás Pancsa
,
Gergely Róbert Nyári
,
Bence Baráth
,
Gábor Cserni
,
Béla Iványi
, and
László Tiszlavicz

-embedded specimens. JCI Insight 2020; 5: e139042. 36 Massoth LR, Desai N, Szabolcs A, et al. Comparison of RNA in situ hybridization and immunohistochemistry

Open access

Background

Fragile histidine triad (FHIT) is a tumor suppressor gene that is commonly inactivated in human tumors. Interestingly, the normal pattern of FHIT expression is largely unknown.

Aim

This study is aimed to characterize the expression of FHIT protein in normal human tissues.

Materials and methods

A total of 119 normal human tissue specimens were analyzed for the FHIT expression using immunohistochemistry technique. The inclusion criteria included: normal/inflammatory tissue with no evidence of cellular atypia.

Results

All studied specimens were stained positively with FHIT and showed either nuclear or cytoplasmic expression. Interestingly, the pattern of FHIT staining was similar among different specimens from each organ. FHIT is located predominantly in the nucleus, although cytoplasmic staining is also present in some cell types. Oral squamous epithelium, breast ductal epithelium, squamous and tubal metaplastic epithelium of the uterine cervix, esophageal squamous epithelium, salivary glands, and bronchial epithelia all strongly expressed the nuclear protein. In connective tissue, FHIT has shown strong cytoplasmic expression in histocytes including macrophages and dendritic cells, fibroblasts, and myofibroblasts.

Conclusion

Documentation of the pattern of FHIT expression in normal tissues will contribute to our understanding of the normal function of this protein and to interpretation of potentially altered FHIT expression in human tumors.

Open access
Acta Veterinaria Hungarica
Authors:
Carmen Solcan
,
Geta Pavel
,
Viorel Floristean
,
Ioan Chiriac
,
Bogdan Şlencu
, and
Gheorghe Solcan

The immunotoxic effect of ochratoxin A (OTA) on the intestinal mucosa-associated lymphoid tissue and its cytotoxic action on the intestinal epithelium were studied in broiler chickens experimentally treated with the toxin. From the 7th day of life, 80 male broiler chickens (Ross 308) were randomly divided into four groups of 20 birds each. The three experimental groups (E1-3) were treated with OTA for 28 days (E1: 50 μg/kg body weight [bw]/day; E2: 20 μg/kg bw/day; E3: 1 μg/kg bw/day) and the fourth group served as control. Histological examination of the intestinal mucosa and immunohistochemical staining for identification of CD4+, CD8+, TCR1 and TCR2 lymphocytes in the duodenum, jejunum and ileocaecal junction were performed, and CD4+/CD8+ and TCR1/TCR2 ratios were calculated. OTA toxicity resulted in decreased body weight gain, poorer feed conversion ratio, lower leukocyte and lymphocyte count, and altered intestinal mucosa architecture. After 14 days of exposure to OTA, immunohistochemistry showed a significant reduction of the lymphocyte population in the intestinal epithelium and the lamina propria. After 28 days of exposure, an increase in the CD4+ and CD8+ values in both the duodenum and jejunum of chickens in Groups E1 and E2 was observed, but the TCR1 and TCR2 lymphocyte counts showed a significant reduction. No significant changes were observed in Group E3. The results indicate that OTA induced a decrease in leukocyte and lymphocyte counts and was cytotoxic to the intestinal epithelium and the mucosa-associated lymphoid tissue, altering the intestinal barrier and increasing susceptibility to various associated diseases.

Open access
Acta Veterinaria Hungarica
Authors:
Katarzyna Paździor-Czapula
,
Joanna Fiedorowicz
, and
Iwona Otrocka-Domagała

Abstract

This study presents a case of a primary hepatic myofibroblastic tumour in a 15-year-old European Shorthair female cat. The cat showed a gradual increase in liver enzymes (alanine aminotransferase and aspartate aminotransferase), and an abdominal ultrasound revealed a tumour located within the left lateral lobe of the liver. The tumour was surgically excised and sent for histopathology. Histopathological examination showed that the tumour was composed of homogeneous fusiform cells with low mitotic count, crowded within the perisinusoidal, portal and interlobular spaces, and entrapment of hepatocytes and bile ducts. Immunohistochemistry revealed that the tumour cells expressed vimentin and α-SMA, and were negative to desmin and cytokeratins. Based on the histological and immunohistochemical features, as well as some similarities with analogous entities in humans and animals, the tumour was classified as a myofibroblastic neoplasm originating from the liver.

Open access
Orvosi Hetilap
Authors:
Helén Hevér
,
Károly Altdorfer
,
Tivadar Zelles
,
Bayarchimeg Batbayar
, and
Erzsébet Fehér

533 548 Kanazawa, H., Yoshie, S.: The taste bud and its innervation in the rat as studied by immunohistochemistry for PGP 9.5. Arch. Histol. Cytol., 1996, 59

Open access
Orvosi Hetilap
Authors:
Anita Sejben
,
László Tiszlavicz
,
József Furák
,
Krisztina Boros
,
Zoltán Sápi
, and
Tamás Zombori

Absztrakt:

A pulmonalis arterialis intimalis sarcoma ritka, magas mortalitású daganat, mely lokalizációja miatt a pulmonalis embolia, valamint a tüdőgyulladás tünettanát utánozhatja. A diagnózis felállítása és a megfelelő kezelés kiválasztása körül számos kérdés merülhet fel. Egy 46 éves, korábban hereseminoma, pulmonalis embolia, asztma, valamint pollenallergia miatt kezelés alatt álló férfi kontroll mellkasi CT-felvételén bal oldalon a mediastinalis pleurát infiltráló, az arteria pulmonalist teljesen elzáró, összességében 7–8 cm-es térfoglaló folyamatot írtak le. A hörgőbiopsziás mintából orsósejtes tumor diagnózisa született, illetve leiomyoma, leiomyomatosus hyperplasia, illetve leiomyosarcoma lehetőségét vetették fel. A fennálló obstrukció miatt a beteg bal oldali tüdőeltávolításon esett át. A szövettani vizsgálat a pulmonalis artériákban terjedő, orsósejtes daganatot kórismézett, immunhisztokémiailag diffúz SMA- és fokális MDM2-pozitivitással, valamint magas proliferációs aktivitással. A h-caldesmon, az S100 protein, az ERG, valamint a pancitokeratin-immunfestések negatívnak bizonyultak. Fluoreszcens in situ hibridizációval a tumorsejtek mintegy 10%-ában polysomiát, illetve MDM2-amplifikációt lehetett igazolni, aminek alapján a „high-grade” pulmonalis arterialis intimalis sarcoma diagnózisa megerősítésre került. Féléves követés alatt a betegség nem újult ki. A pulmonalis arterialis intimalis sarcoma pontos incidenciája nem ismert. Egyes források szerint a krónikus pulmonalis hypertoniás betegek 1–4%-ánál fordul elő. A tünetek közül a fogyás kelti fel a leginkább a daganatos betegség gyanúját. A képalkotó és a kórszövettani vizsgálatoknak kulcsszerepük van a diagnózis felállításában. A daganat rossz kórjóslata miatt a minél korábbi felismerés és a kemoterápiával kombinált sebészi kezelés javíthatja a túlélési lehetőségeket. Orv Hetil. 2020; 161(6): 232–236.

Open access

A tüdő histiocytás sarcomája

Pulmonary histiocytic sarcoma

Orvosi Hetilap
Authors:
Balázs Vincze
,
Erika Tóth
,
Laura Iski
,
Péter Mezei
,
Károly Szabó
,
László Országh
,
Judit Kovács
,
Zoltán Szöllősi
,
Éva Szövördi
, and
Imre Tóth

A histiocytás sarcoma ritka, malignus vérképző szervi daganat. Tüdőben való előfordulása nagyon ritka. Az esetek kis száma, valamint a betegség klinikai és patológiai jellegzetességei miatt diagnosztikus kihívást jelenthet. Optimális kezelése egyelőre nem ismert, lokális esetekben – az elhelyezkedéstől és a mérettől függően – a sebészeti eltávolítás a komplex onkoterápia részét képezi. Egy 52 éves férfi beteg esetét ismertetjük, akinél a bal tüdőben centrálisan elhelyezkedő daganat miatt pulmonectomiát végeztünk. A szövettan histiocytás sarcomát igazolt. Az eset kapcsán rövid irodalmi áttekintést nyújtunk a betegség klinikumáról. Orv Hetil. 2023; 164(34): 1350–1357.

Open access