Authors:Matthias Halfter, Ulrich Müseler, Ralf Matthias Hagen, and Hagen Frickmann
prospective studies are desirable to further assess specific health risks for police officers on tropical deployments. Conclusions In spite of the limitations mentioned above, the study indicates a quantitatively relevant minority of infected and infested
Authors:Konstantin Tanida, Lars Koste, Christian Koenig, Werner Wenzel, Andreas Fritsch, and Hagen Frickmann
To evaluate the automated cartridge-based PCR approach ARIES SARS-CoV-2 Assay targeting the ORF-sequence and the N-gene of SARS-CoV-2.
In line with the suggestions by Rabenau and colleagues, the automated ARIES SARS-CoV-2 Assay was challenged with strongly positive samples, weakly positive samples and negative samples. Further, intra-assay and inter-assay precision as well as the limit-of-detection (lod) were defined with quantified target RNA and DNA. The Cepheid Xpert Xpress SARS-Cov-2 Assay was used as gold standard.
Concordance between the ARIES assay and the Cepheid assay was 100% for strongly positive samples and for negative samples, respectively. For weakly positive samples as confirmed applying the Cepheid assay, a relevant minority of 4 out of 15 samples (26.7%) went undetected by the ARIES assay. Intra- and inter-assay precision were satisfactory, while the lod was in the 103 DNA copies/reaction-range, in the 103 virus copies/reaction-range, or in the 103–104 free RNA copies/reaction-range in our hands.
The automated ARIES assay shows comparable test characteristics as the Cepheid assay focusing on strongly positive and negative samples but a slightly reduced sensitivity with weakly positive samples. Decisions on diagnostic use should include considerations on the lod.
Authors:Carina Gottwald, Norbert Georg Schwarz, and Hagen Frickmann
prevalence for HIV (0.06%), syphilis (0.65%), HCV (0.82%), and HSV-2 (3.03%) was documented in spite of a quantitatively relevant minority reporting sexual risk practices like paying women for sex (21.3%) and sex between males (also 21.3%) [ 15 ]. In a Korean
instance, invasive bacteria) frequently employ specific strategies to reach deeper tissue. In the natural course of events, immune cells will detect the invaders and deal with them. In a minority of cases, bacteria may reach the bloodstream, and if they
Authors:K. Hoenes, M. Hess, P. Vatter, B. Spellerberg, and M. Hessling
S. cerevisiae are 182 J/cm 2 and 526 J/cm 2 for 405 nm and 450 nm, respectively. The trypan blue experiments show that only a minority of S. cerevisiae are stained, indicating an intact cell membrane and thus contradicting the previously
Authors:N. von Allmen, K. Gorzelniak, O. Liesenfeld, M. Njoya, J. Duncan, E. M. Marlowe, T. Hartel, A. Knaust, B. Hoppe, and M. Walter
as an add-on to culture-based testing; however, rapid PCR test results should be labeled as preliminary until confirmed by culture. The requirement for rapid PCR-based testing plus culture-based confirmation—while only applied in a minority of
Authors:Isabel Stephany-Brassesco, Stefan Bereswill, Markus M. Heimesaat, and Matthias F. Melzig
proven challenging. Obtaining samples from the lung is difficult and invasive, and biopsies are only performed in a minority of cases. Most bacteria linked to pneumonia are commonly found among the commensal flora of the upper respiratory tract, so how
Authors:Hans Kollenda, Hagen Frickmann, Rania Ben Helal, Dorothea Franziska Wiemer, Habiba Naija, Mohamed Sélim El Asli, Melanie Egold, Joachim Jakob Bugert, Susann Handrick, Roman Wölfel, Farouk Barguellil, and Mohamed Ben Moussa
Background: Carbapenem-resistance is frequently detected in Enterobacteriaceae isolated from patients in Tunisia. The study was performed to identify frequent carbapenemases in Tunisian isolates.
Methods: Between May 2014 and January 2018, 197 ertapenem-resistant Enterobacteriaceae were isolated at the microbiological department of the Military Hospital of Tunis. The strains were phenotypically characterized and then subjected to in-house polymerase chain reaction (PCR) targeting the carbapenemase genes blaIMP, blaVIM, blaNDM, blaSPM, blaAIM, blaDIM, blaGIM, blaSIM, blaKPC, blaBIC, and blaOXA-48.
Results: The assessed 197 ertapenem-resistant Enterobacteriaceae from Tunis comprised 170 Klebsiella pneumoniae, 19 Enterobacter cloacae, 6 Escherichia coli, 1 Citrobacter sedlakii, and 1 Enterobacter asburiae. Thereby, 55 out of 197 isolates (27.9%) were from blood cultures, suggesting a systemic disease. The carbapenemase gene blaOXA-48 quantitatively dominated by far with 153 detections, followed by blaNDM with 14 detections, which were distributed about the whole study interval. In contrast, blaBIC and blaVIM were only infrequently identified in 5 and 3 cases, respectively, while the other carbapenamases were not observed.
Conclusions: The carbapenemase gene blaOXA-48 was identified in the vast majority of ertapenem-resistant Tunisian Enterobacteriaceae while all other assessed carbapenemases were much less abundant. In a quantitatively relevant minority of isolates, the applied PCR-based screening approach did not identify any carbapenemases.