Authors:É. Szőke, G. Petroianu, K. Tekes, B. Benkő, P. Szegi, R. Laufer, and G. Veress
Reversed-phase HPLC has been used to monitor the concentration of the two major Chamomile components rutin and quercetin during rat liver microsomal treatment. The possibility of microsomal oxidative metabolism or stability of these two components was examined using a guard-column without any clean-up. The concentration of quercetin decreased when exposed to rat liver microsomal media whereas the rutin concentration did not change significantly over one hour of treatment.
An ultrasensitive and rapid method for the determination of epicatechin, rutin, and quercetin was developed using capillary zone electrophoresis with on-line chemiluminescence detection. Under the optimal conditions, the analytes were baseline separated within 12 min. The limits of detection in turn were 0.60 pg mL−1 for epicatechin, 0.50 pg mL−1 for rutin, and 1.0 pg mL−1 for quercetin. The developed method was an easy and reliable method of determining these analytes concentrations in tea, extract Ginkgo biloba, and rutin tablet, demonstrating the feasibility and reliability of the proposed method.
Authors:Bingying Hu, Yingying Sun, Min Wang, Zhisheng He, Shanshan Chen, Dake Qi, Zhen Ge, Lingling Fan, Jingfang Chen, and Yang Wei
glycoside prototypes and a pharmacokinetic research of them were needed. Rutin was an abundant flavonol glycoside of GBE [ 13 , 14 ] and was selected as a representative flavonol glycoside to be investigated in this study.
To quantify the rat
Authors:Y.T. Kamal, Sayeed Ahmad, Nanjaian Mahadevan, Prawez Alam, Shahana Salam, Yahya I Asiri, Abdullatif Bin Muhsinah, and Abdulrhman Alsayari
free flavonoids like rutin and quercetin forms the basis of bioactivity of these Itrifal formulations [ 5 ]. The anthraquinone glycosides like sennoside A and B were selected as a specific marker for C. angustifolia , which is present in a significant
from Sigma-Aldrich, St. Louis (Missouri, USA). Precoated TLC (silica gel 60 F254) and quercetin were purchased from Merck (Darmstadt, Germany). Rutin and β-sitosterol were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). The solvents used for
Authors:Yuping Shen, Minhui Xu, Peipei Deng, Qinying Gu, Huawu Yin, Guohua Xia, Xiaobin Jia, Huan Yang, and James Tam
quality of bioactive chemical constituents in natural products [ 15 ]. Accordingly, in our previous studies, gallic acid, methyl gallate, and a few non-specific flavonoids such as rutin, kaempferol 3- O - β - d -glucopyranoside, and quercitrin, in Red Toon
Authors:Omar M. Khalaf, Mosad A. Ghareeb, Amal M. Saad, Hassan M. F. Madkour, Ahmed K. El-Ziaty, and Mohamed S. Abdel-Aziz
:9, v/v), followed by PTLC eluted with (ethyl acetate: MeOH; 2.1:2.9; v/v) to give quercetin 3- O -α- l -rhamnopyranosyl-(1→6)-β- d -glucopyranoside (rutin) ( 5 ), while fraction (V) was purified on silica gel sub-column eluted with (EtOAc–MeOH; 17: 3, v
Authors:G.-F. Shi, Z.-J. Wang, G.-Y. Wang, R.-X. Yao, F.-W. Chen, and Z.-R. Shi
High-performance liquid chromatography-mass spectrometry (HPLC-MS) method coupled with radical reaction for screening active ingredients from perennial fujimoto bean whole herb was established. The active ingredients, present in perennial fujimoto bean whole herb, possess scavenging effects towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide, peroxy radical, and hydroxyl radical. The radical scavenging abilities of these active ingredients were evaluated based on the relative peak areas in the HPLC chromatogram. The results indicate that potent antioxidants are present in the anhydrous methanol extract of perennial fujimoto bean whole herb. Based on HPLC-MS analysis, it was found that the scavenging ability can be mostly attributed to the presence of three compounds: cyanidin-3-o-β-d-glucopyranoside, troxerutin, and rutin. The structures were identified based on the MS and nuclear magnetic resonance (NMR) data. Free radical scavenging activity decreased in the following order: troxerutin > rutin > cyanidin-3-o-β-d-glucopyranoside.