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A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 μg/g for cotinine, 20 μg/g for anabasine and nornicotine, and 30 μg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

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Acta Chromatographica
Authors: Lianguo Chen, Qingwei Zhang, Yijing Lin, Xiaojie Lu, Zuoquan Zhong, Jianshe Ma, Congcong Wen, and Cheng Ding

necessary to establish an analytical method to monitor the concentration of hapepunine. To the best of our knowledge, the pharmacokinetics of hapepunine had not been reported. In this paper, an ultra-performance liquid chromatography–tandem mass

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spectrometry (MS) [ 18 , 19 ], or coupled to tandem mass spectrometry (MS/MS) [ 6 , 18 , 20 – 23 ], had become the best choice for the determination of CAP because of its advantages of high selectivity and sensitivity, timesaving, and the accuracy of

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/sample. The injection volume was 10 μL. Electrospray ionization–tandem mass spectrometry (ESI–MS/MS) detection was achieved on an Agilent 6410 triple quadrupole tandem mass spectrometer. The positive ionization mode was used, and the ions were

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. Therefore, we attempted to develop and validate a sensitive and specific analytical method for the simultaneous determination of ZnPT and PT in shampoos using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Experimental

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]. LC coupled to an ultraviolet (UV) source or diode array [ 16 , 18 , 19 ], fluorescence [ 20 ], a time-of-flight (TOF) analyzer [ 21 ], or biosensor [ 22 ] has been used to detect antibiotics. Meanwhile, LC coupled to tandem mass spectrometry (LC

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Acta Chromatographica
Authors: Ruijie Chen, Mengrou Lu, Xiaoting Tu, Wei Sun, Weijian Ye, Jianshe Ma, Congcong Wen, Xianqin Wang, and Peiwu Geng

]. There is no effective bioanalytical method available to quantify panasenoside in biological samples to date. Compared to liquid chromatography tandem mass spectrometry (LC–MS/MS), ultra-performance liquid chromatography (UPLC)–MS/MS is more sensitive and

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humans, they both used [ 14 C]-labeled apremilast rather than normal tablets [ 15 ]. Liu’s method has been used to study the drug interactions with apremilast by liquid chromatography-tandem mass spectrometry [ 16, 17 ]. However, no study has reported

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quadrupole MS stands out as a fast, sensitive, and accurate quantification method for complex systems, such as natural product extracts [ 10 , 11 ]. The goal of the present study was to develop and validate a method coupling HPLC with tandem mass

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The rhizome of Sparganium stoloniferum Buch.-Ham has been used as a traditional Chinese folk medicine for thousands of years. Phenolic compounds are the main bioactive ingredients of the plant. In order to determine the content of phenolic compounds from different major cultivations, a reliable method has been developed using ultra-high performance liquid chromatography coupled with a triple quadrupole electrospray tandem mass spectrometry. Seven compounds, including rutin, kaempferol, p-hydroxybenzaldehyde, formononetin, ferulic acid, vanillic acid, and p-coumaric acid, were simultaneously measured in 10 min. The established approach was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery, and successfully applied to determine seven phenolic compounds of Rhizoma Sparganii. This study may be helpful in the quality control of Rhizoma Sparganii and can offer technical support for the pharmacological and clinical study of related drugs.

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