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Journal of Behavioral Addictions
Authors: Mitra-Sadat Sadat-Shirazi, Nasim Vousooghi, Bentolhoda Alizadeh, Seyed Mohammad Makki, Seyed Zeinolabedin Zarei, Shahrzad Nazari, and Mohammad Reza Zarrindast

Biosystems, USA). For each gene, the real-time PCR reaction was adjusted in such a way that a single product was amplified for each primer pair. The optimized annealing temperature of reactions was from 60 to 63 °C. Observing a single peak in melting curve

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