Authors:C.L. Liu, X.Y. Wang, Z.M. Wang, S.S. Li, C.T. Xin, H.F. Wang, B. Li, and L. Jiang
The migration of 99Tc in a weak loess aquifer was investigated in-situ with undisturbed aquifer medium columns. The columns were obtained horizontally at a depth of 3236 m in an Underground Research Facility (URF). Quartz containing 3H (HTO) and 99Tc (in the form of 99TcO4-) was introduced into one end of the columns and the columns were covered tightly. Aquifer water was introduced into the columns directly from an experimental shaft in the UFR. Effluents from the columns were collected and the activity of 3H and 99Tc were determined with a liquid scintillation analyzer. The breakthrough curves of 3H and 99Tc indicate that 99Tc migrates a little faster than that 3H does in the aquifer.
Authors:X. Han, Y. Sun, T. Wang, Zh. Lin, Sh. Li, F. Zhao, Z. Liu, J. Yi, and X. Ren
The effects of fullerenes, including fellerene soot (FS), extracted fullerene soot (EFS) and pure C60 on the thermal decomposition of ammonium perchlorate (AP) compared with traditional carbon black (CB) catalyst has been studied
by employing thermogravimetry (TG), differential thermal analysis (DTA), infrared spectroscopy (IR) and ignition temperature
experiments. The results showed that the addition of CB and FS to AP reduced the activation energy as well as the temperature
at maximum decomposition rate, but that of EFS and pure C60 had little effect on the thermal decomposition of AP, and among all catalysts, FS was the best one.
Authors:M.Y. Jiang, Z.R. Wang, K.W. Chen, J.Q. Kan, K.T. Wang, Zs. Zalán, F. Hegyi, K. Takács, and M.Y. Du
After suffering from mechanical injury and fungal infection, grapes are perishable. Botrytis cinerea, the causal agent of gray mould, is a critical pathogen for grapes. In this study, the inhibitory effect of Pseudomonas fluorescens on the formation of gray mould on grapes during the postharvest storage was investigated on “Kyoho” grape. The results suggest that a living cell suspension of P. fluorescens significantly inhibited spore germination of B. cinerea, and significantly reduced the incidence of grape gray mould. Moreover, compared with the control, the fruit inoculated with P. fluorescens had elevated activities of polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT), phenylalanine ammonia-lyase (PAL), chitinase (CHI), and β-1,3-glucanase (GLU). Increase in enzyme activity correlated with enhanced host resistance. In addition, there was little difference in storage quality between the treated group and control group, indicating no adverse effects of the induced defence response on fruit quality.
Authors:G. Vida, M. Cséplő, G. Gulyás, I. Karsai, T. Kiss, J. Komáromi, E. László, K. Puskás, Z. Wang, C. Pace, Z. Bedő, L. Láng, and O. Veisz
Among the factors which determine yield reliability an important role is played by disease resistance. One of the breeding aims in the Martonvásár institute is to develop wheat varieties with resistance to major diseases. The winter wheat varieties bred in Martonvásár are examined in artificially inoculated nurseries and greenhouses for resistance to economically important pathogens. The effectiveness of designated genes for resistance to powdery mildew and leaf rust has been monitored over a period of several decades. None of the designated major resistance genes examined in greenhouse tests is able to provide complete resistance to powdery mildew; however, a number of leaf rust resistance genes provide full protection against pathogen attack (Lr9, Lr19, Lr24, Lr25, Lr28 and Lr35). In the course of marker-assisted selection, efficient resistance genes (Lr9, Lr24, Lr25 and Lr29) have been incorporated into Martonvásár wheat varieties. The presence of Lr1, Lr10, Lr26, Lr34 and Lr37 in the Martonvásár gene pool was identified using molecular markers. New sources carrying alien genetic material have been tested for powdery mildew and leaf rust resistance. Valuable Fusarium head blight resistance sources have been identified in populations of old Hungarian wheat varieties. Species causing leaf spots (Pyrenophora tritici-repentis, Septoria tritici and Stagonospora nodorum) have gradually become more frequent over the last two decades. Tests on the resistance of the host plant were begun in Martonvásár four years ago and regular greenhouse tests on seedlings have also been initiated.
Aegilops sharonensis (Sharon goatgrass) is a valuable source of novel high molecular weight glutenin subunits, resistance to wheat rust, powdery mildew, and insect pests. In this study, we successfully hybridized Ae. sharonensis as the pollen parent to common wheat and obtained backcross derivatives. F1 intergeneric hybrids were verified using morphological observation and cytological and molecular analyses. The phenotypes of the hybrid plants were intermediate between Ae. sharonensis and common wheat. Observations of mitosis in root tip cells and meiosis in pollen mother cells revealed that the F1 hybrids possessed 28 chromosomes. Chromosome pairing at metaphase I of the pollen mother cells in the F1 hybrid plants was low, and the meiotic configuration was 25.94 I + 1.03 II (rod). Two pairs of primers were screened out from 150 simple sequence repeat markers, and primer WMC634 was used to identified the presence of the genome of Ae. sharonensis. Sequencing results showed that the F1 hybrids contained the Ssh genome of Ae. sharonensis. The sodium dodecyl sulfate polyacrylamide gel electrophoresis profile showed that the alien high molecular weight glutenin subunits of Ae. sharonensis were transferred into the F1 and backcross derivatives. The new wheat-Ae. sharonensis derivatives that we have produced will be valuable for increasing resistance to various diseases of wheat and for improving the quality of bread wheat.
Authors:W.M. Du, F.H. Wang, H.Y. Zhang, B.Z. Jiang, X.Y. Chen, W. Zhang, Y. Xie, and Z.T. Sheng
Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confirmed that the growth of both beneficial microbe B. longum subsp. longum (0.695, 0.205: R2, slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R2, slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, –0.165: R2, slope, respectively) growth. In vivo studies also revealed that genome copies of Bifidobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment.
Authors:B.L. Béres, N.Z. Lupwayi, F.J. Larney, B. Ellert, E.G. Smith, T.K. Turkington, D. Pageau, K. Semagn, and Z. Wang
Research indicates that not all crops respond similarly to cropping diversity and the response of triticale (× Triticosecale ssp.) has not been documented. We investigated the effects of rotational diversity on cereals in cropping sequences with canola (Brassica napus L.), field pea (Pisum sativum L.), or an intercrop (triticale:field pea). Six crop rotations were established consisting of two, 2-yr low diversity rotations (LDR) (continuous triticale (T-T_LDR) and triticale-wheat (Triticum aestivum L.) (T-W_LDR)); three, 2-yr moderate diversity rotations (MDR) (triticale-field pea (T-P_MDR), triticale-canola (T-C_MDR), and a triticale: field pea intercrop (T- in P_MDR)); and one, 3-yr high diversity rotation (HDR) (canola-triticale-field pea (C-T-P_HDR)). The study was established in Lethbridge, Alberta (irrigated and rainfed); Swift Current (rainfed) and Canora (rainfed), Saskatchewan, Canada; and carried out from 2008 to 2014. Triticale grain yield for the 3-yr HDR was superior over the LDR rotations and the MDR triticale-field pea system; however, results were similar for triticale-canola, and removal of canola from the system caused a yield drag in triticale. Triticale biomass was superior for the 3-yr HDR. Moreover, along with improved triticale grain yield, the 3-yr HDR provided greater yield stability across environments. High rotational diversity (C-T-P_HDR) resulted in the highest soil microbial community and soil carbon concentration, whereas continuous triticale provided the lowest. Net economic returns were also superior for C-T-P_HDR ($670 ha–1) and the lowest for T-W_LDR ($458 ha–1). Overall, triticale responded positively to increased rotational diversity and displayed greater stability with the inclusion of field pea, leading to improved profitability and sustainability of the system.
Authors:W.T. Xue, A. Gianinetti, R. Wang, Z.J. Zhan, J. Yan, Y. Jiang, T. Fahima, G. Zhao, and J.P. Cheng
Crop seeds are the main staples in human diet, especially in undeveloped countries. In any case, the diet needs to be rich not only in macro-nutrients like carbohydrates and protein, but also in micro-nutrients. Nevertheless, both the macro- and micro-nutrients presented in seeds largely vary in consequence of field and environment conditions. In this research, 60 lines of a barley RILs population segregating for the SSR marker Hvm74, which is genetically linked to the GPC (grain protein content) locus (HvNAM-1), were studied in 4 environments (two growing years and two field managements) by carrying out a comprehensive profile of seed macro- (starch, total nitrogen and total soluble protein) and micro-nutrients (phytate, phenolics, flavonoids, Pi, Zn and Fe). Under field conditions, all the components were largely affected by the environment, but TN (total nitrogen) exhibited high genotype contribution, while micro-nutrients displayed higher genotype × environments interactions (GEI) than macro-nutrients. In order to approach the effects of carbon-nitrogen (C–N) balance on other seed components, two C/N ratios were calculated: C/TN (CNR1) and C/TSP (CNR2). CNR2 exhibited stronger negative correlations with all micro-nutrients. Hence, the significant GEI and its negative relationships with CNR2 highlighted the different characters of micro-nutrients in barley seeds.
Authors:Y.P. Jing, D.T. Liu, X.R. Yu, F. Xiong, D.L. Li, Y.K. Zheng, Y.F. Hao, Y.J. Gu, and Z. Wang
The objective of the present study was to understand the developmental regularity of wheat endosperm cells at different Days After Pollination (DAP) using microscopic and histochemical methods. Resin semi-thin sections of the endosperm and the enzymatically dissociated Starchy Endosperm Cells (SECs) were observed under a light microscope. The results showed that: (1) SECs were irregular-shaped and had two types of starch granules: large oval-shaped A-type starch granules and small spherical B-type starch granules. (2) The growth shape of SECs was referred to as S-curve and the fastest cell growth period was at 16–24 DAP. (3) The largest increase and growth of A-type starch granules were mainly at 4–16 DAP. B-type starch granules increased rapidly after 16 DAP and made up over 90% of the total starch granules in SEC during the late stage of endosperm development. (4) The nuclei of SEC deformed and degenerated during the middle and late stages of endosperm development and eventually disappeared. However, starch granules still increased and grew after the cell nuclei had degenerated. The investigations showed the development regularity of starch endosperm cells and starch granules, thereby improving the understanding of wheat endosperm development.
Authors:Y. C. Xiao, L. T. Liu, J. J. Bian, C. Q. Yan, L. Ye, M. X. Zhao, Q. S. Huang, W. Wang, K. Liang, Z. F. Shi, and X. Ke
Shuganjieyu (SGJY) capsule is a classical formula widely used in Chinese clinical application. In this paper, an ultra-performance liquid chromatography coupled with electrospray ionization and ion trap mass spectrometry has been established to separate and identify the chemical constituents of SGJY and the multiple constituents of SGJY in rats. The chromatographic separation was performed on a C18 RRHD column (150 × 2.1 mm, 1.8 μm), while 0.1% formic acid–water and 0.1% formic acid–acetonitrile was used as mobile phase. Mass spectral data were acquired in both positive and negative modes. On the basis of the characteristic retention time (Rt) and mass spectral data with those of reference standards and relevant references, 73 constituents from the SGJY and 15 ingredients including 10 original constituents and 5 metabolites from the rat plasma after oral administration of SGJY were identified or tentatively characterized. This study provided helpful chemical information for further pharmacology and active mechanism research on SGJY.