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  • Author or Editor: Zheng Yu x
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Xinjiang rice wheat ( Triticum petropavlovskyi Udacz. et Migush, 2n=6x=42, AABBDD) is one of the endemic Chinese wheats, only distributing in Xinjiang and Xizang (Tibet), China. A novel high-molecular-weight (HMW) glutenin subunit gene 1Dx2.1 was isolated and characterized from Xinjiang rice wheat accession Daomai2. The complete open reading frame (ORF) of 1Dx2.1 is 2508 bp, encoding 836 amino acids. The primary structure of 1Dx2.1 consists of three distinct domains, a non-repetitive N-terminal domain with 89 residues, a non-repetitive C-terminal domain with 42 residues and a large central repetitive domain with 684 residues. In the N-terminal of 1Dx2.1, there is an R (arginine) at position 75, whereas there is a Q (glutamine) in other known x-type subunits. Four cysteine residues are observed in 1Dx2.1 with three in the N-terminal region and one in the C-terminal region. The number and distribution of cysteines in 1Dx2.1 are identical to those in x-type subunits except for 1Dx5, which possesses an extra cysteine residue. Differences between the repetitive domain of 1Dx2.1 and those of known HMW subunits resulted from substitutions, insertions or/and deletions involving single or more amino acid residues. The phylogenetic tree, which was constructed on the basis of amino acid sequences, and indicated that 1Dx2.1 was highly related to 1Dx2.1 t , then to 1Dx2 and 1Dx5.

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Abstract

Calycanthine is an important class of alkaloids extracted and isolated from the roots, leaves, flowers and fruits of Chimonanthus praecox. In this work, the UPLC-MS/MS method was used for determination of calycanthine in rat plasma, and the pharmacokinetics in rats were investigated. Midazolam was used as an internal standard (IS), and methanol precipitation method was used to pretreatment the rat plasma samples. Chromatographic separation was achieved on a UPLC BEH C18 (50 × 2.1 mm, 1.7 μm) column with the mobile phase of methanol- 0.1% formic acid aqueous solution with gradient elution. Multiple reaction monitoring (MRM) mode with positive ionization was applied for quantitative analysis, m/z 347.3 → 246.7 and 326.2 → 291.4 for calycanthine and IS, respectively. The results indicated that within the range of 1–200 ng/mL, linearity of calycanthine in rat plasma was good (r > 0.995), and the lower limit of quantification (LLOQ) was 1 ng/mL. Accuracy range was between 90.6 and 109.4%, precision (RSD) of calycanthine was less than 14%. The matrix effect was between 97.9% and 105.4%, the recovery was better than 85.6%. The developed UPLC-MS/MS method was successfully applied in the pharmacokinetics of calycanthine in rats after oral and intravenous administration. The absolute bioavailability of the calycanthine was 37.5% in rats.

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The aim of this study was to investigate postprandial effects of two Chinese liquors on s elected cardiovascular disease risk factors in humans. Sixteen healthy men were randomized into three groups in a three-way crossover study: tea-flavor liquor (TFL), traditional Chinese liquor (TCL) and water control (WC). Every subject consumed 60 mL of either liquor (45% (v/v) ethanol) or water together with a high-fat meal, respectively. Compared with baseline, serum uric acid was significantly increased in TFL group (0.5-hour: P = 0.012; 1-hour: P = 0.001; 2-hour: P = 0.008) and it was significantly decreased in WC group (1-hour: P = 0.001; 2-hour: P = 0.001; 4-hour: P < 0.001), while uric acid was non-significantly increased in the TCL group. High-sensitive C-reactive protein (hs-CRP) was significantly increased in the TCL (P = 0.014) and WC (P = 0.008) groups at postprandial 4 hours compared with baseline. There was no significant difference between groups during the postprandial period for these two parameters or other biochemical parameters. In conclusion, both liquors increased postprandial uric acid, and no significant difference was observed for the effects of TFL and TCL on the measured biochemical parameters.

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Abstract  

The objective of this study was to evaluate the potential ecological danger and toxic effect of uranium mill tailings leaching solution (UMTLS) on aquatic animals. UMTLS was identified to contain two radioactive elements, nine heavy metal elements, and five non-metallic materials. The acute toxicity test indicated that the 1, 12, 24, 48, 72, 96 h LC50 values of UMTLS to the zebrafish were 12.1, 7.1, 4.4, 3.8, 3.4, and 2.9%, respectively. In sub-lethal toxicity tests, superoxide dismutase, catalase, Na+–K+–ATPase activities, and malondialdehyde content were respectively determined and analyzed in the zebrafish gill, gonad, muscle, and liver after exposed to four different concentration levels of UMTLS for 7 and 14 days, respectively. The result showed that the most sensitivity of the antioxidant system in zebrafish tissues in UMTLS was gill, and then decreased in gonad, muscle and liver respectively. Na+–K+–ATPase activity in the liver and gonad may be considered as a reference biomarker of UMTLS stress. The data in this study may be valuable that the toxicity of such as the leaching solution of potentially hazardous material was compared with that of each constituent.

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The aim of this study was to investigate the effects of maternal lead exposure on the learning and memory ability and expression of tau protein phosphorylation (P-tau) and beta amyloid protein (Aβ) in hippocampus of mice offspring. Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups. On the 21 th of postnatal day, the learning and memory ability of the mouse pups was tested by Water Maze test and the Pb levels in blood and hippocampus of the offspring were also determined. The expression of P-tau and Aβ in hippocampus was measured by immunohistochemistry and Western blotting. The Pb levels in blood and hippocampus of all exposure groups were significantly higher than that of the control group ( P < 0.05). In Water Maze test, the performances of 0.5% and 1% groups were worse than that of the control group ( P < 0.05). The expression of P-tau and Aβ was increased in Pb exposed groups than that of the control group ( P < 0.05). Tau hyper-phosphorylation and Aβ increase in the hippocampus of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.

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Journal of Radioanalytical and Nuclear Chemistry
Authors: Jiaoyun Xia, Yongxian Wang, Junfeng Yu, Shiqiang Li, Lin Tang, Mingqiang Zheng, Xiuqing Liu, Gucai Li, Dengfeng Cheng, Sheng Liang, and Duanzhi Yin

Abstract  

Radiolabeling of biologically active molecules with fac-[188Re(CO)3(H2O)3]+ unit has been of primary interest in recent years. Therefore, we herein report ligands L1−L4 (L1=histidine, L2=nitrilotriacetic acid, L3=2-picolylamine-N,N-diacetic acid, L4=bis(2-pyridymethy)amine) that have been evaluated by radiochemical reactions with fac-[188Re(CO)3(H2O)3]+. These reactions yielded the radioactive complexes of fac-[188Re(CO)3L] (L = L1−L4, 188Re tricarbonyl complexes 1–4), which were identified by HPLC. Complexes 1–4, with log P o/w values ranging from −2.23 to 2.18, were obtained with yields of ≥95% using ligand concentrations within 10−6–10−4M range. Thus, specific activities of 220 GBq/μmol could be achieved. Challenge studies with cysteine and histidine revealed high stability for all of these radioactive complexes, and biodistribution studies in mice indicated a fast rate of blood clearance and high rate of total radioactivity excretion occurring primarily through the renal-urinary pathway. In summary, the ligands L1–L4 are potent chelators for the future functionalization of biomolecules labeling with fac-[188Re(CO)3(H2O)3]+.

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Scientometrics
Authors: Jia Zheng, Zhi-yun Zhao, Xu Zhang, Dar-zen Chen, Mu-hsuan Huang, Xiao-ping Lei, Ze-yu Zhang, Yun-hua Zhao, and Run-sheng Liu

Abstract

Patents are the manifestation of the industry's research and development (R&D) endeavor; therefore, this paper studies the industry evolution of and key technologies in China from the perspective of patent analysis. Patents in six types of industries, including Chemical (excluding Drugs), Computers and Communications, Drugs and Medical, Electrical and Electronics (E&E), Mechanical, and Others are analyzed in this study. Findings from the analysis show a steady increase of US granted utility patents in China as well as percentage of these patents in the world over the period between 2003 and 2008. All the above industries in China have been growing rapidly during this period, which is very different from the global industry development. Despite the rapid development, the citation rates of these patents have been low, reflecting a need for improvement in the quality of patents and R&D performance for these six industries in China in order to exert more influence in the industry world. The analysis on patents also reveals China's industry distribution to be similar to the global industry distribution, with the exception of E&E industry which weights over one third of the total patents in technologies. The E&E industry is also the field with largest economic growth which rises more rapidly after 2006 with a sudden increase of patents in USPC 361. Detailed tracking of the key technology evolution reveals that 90% of the newly issued patents in USPC 361 after 2006 are owned by Foxconn Technology Co., Ltd, pointing to an unbalanced R&D environment in China's E&E industry sector. By providing the insight into the evolution of China's industrial and technological development through the perspective of patent analysis, this paper hopes to provide an objective statistic reference for future policy directions and academic researches.

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Acta Chromatographica
Authors: Jianbo Li, Zheng Yu, Cheng Han, Zhening Wang, Yujie Hu, Congcong Wen, and Chongliang Lin

In this study, we used UPLC–MS/MS to determine diosmetin-7-o-β-d-glucoside in rat plasma and investigated its pharmacokinetics in rats. Six rats were given diosmetin-7-o-β-d-glucoside (5 mg/kg) by intravenous (i.v.) administration. The blood (150 μL) was withdrawn from the caudal vein after administration. Diazepam was used as an internal standard (IS), and a one-step acetonitrile precipitation method was used to process the plasma samples. Chromatographic separation was achieved using a UPLC BEH C18 column using a mobile phase of acetonitrile–0.1% formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization was applied, 463.1 → 301.0 for diosmetin-7-o-β-d-glucoside, m/z 285.1 → 193.0 for diazepam (IS). Intra-day and inter-day precision of diosmetin-7-o-β-d-glucoside in rat plasma were less than 14%. The method was successfully applied in the pharmacokinetics of diosmetin-7-o-β-d-glucoside in rats after intravenous administration. The t 1/2 of diosmetin-7-o-β-d-glucoside is 1.4 ± 0.4 h, which indicates the quick elimination.

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Cereal Research Communications
Authors: Y.P. Jing, D.T. Liu, X.R. Yu, F. Xiong, D.L. Li, Y.K. Zheng, Y.F. Hao, Y.J. Gu, and Z. Wang

The objective of the present study was to understand the developmental regularity of wheat endosperm cells at different Days After Pollination (DAP) using microscopic and histochemical methods. Resin semi-thin sections of the endosperm and the enzymatically dissociated Starchy Endosperm Cells (SECs) were observed under a light microscope. The results showed that: (1) SECs were irregular-shaped and had two types of starch granules: large oval-shaped A-type starch granules and small spherical B-type starch granules. (2) The growth shape of SECs was referred to as S-curve and the fastest cell growth period was at 16–24 DAP. (3) The largest increase and growth of A-type starch granules were mainly at 4–16 DAP. B-type starch granules increased rapidly after 16 DAP and made up over 90% of the total starch granules in SEC during the late stage of endosperm development. (4) The nuclei of SEC deformed and degenerated during the middle and late stages of endosperm development and eventually disappeared. However, starch granules still increased and grew after the cell nuclei had degenerated. The investigations showed the development regularity of starch endosperm cells and starch granules, thereby improving the understanding of wheat endosperm development.

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Acta Chromatographica
Authors: Qinghua Weng, Lianguo Chen, Luxin Ye, Xiaojie Lu, Zheng Yu, Congcong Wen, Yichuan Chen, and Gang Huang

The aim of this study was to establish a rapid, sensitive, and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method to quantify the concentrations of licochalcone A and applicate the technique to its pharmacokinetic study. Analytes were separated on an UPLC ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm, 1.7 μm). The mobile phase was consisted of acetontrile and 0.1% formic acid with a flow rate of 0.4 mL/min in a gradient elution mode. Multiple-reaction monitoring (MRM) was carried out in a negative mode for licochalcone A (m/z 337.2 → 119.7) and the internal standard (IS) (m/z 609.0 → 300.9). The linearity of licochalcone A was great from 0.53 to 530 ng/mL. The lower limit of quantification and the lower limit of detection were 0.53 ng/mL and 0.26 ng/mL, respectively. The intra-day precision was less than 14%, and the inter-day precision was no more than 11%. The accuracy was from 91.5% to 113.9%, the recovery was over 90.5%, and the matrix effect was between 84.5% and 89.7%. The results of stability were in an acceptable range. The bioavailability was only 3.3%, exhibiting poor absorption. The developed method was successfully applicable for determining the concentrations of licochalcone A and its pharmacokinetic study.

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