Authors:X. P. Geng, H. F. Zhang, B. H. Wang, X. D. Geng, and J. W. Xing
Calorimetric determination of the total enthalpy changes (ΔHi) of guanidine-denatured lysozyme (Lys) during the adsorption with simultaneously refolding on the surface of hydrophobic
interaction chromatography packings was carried out at 250.001C. The measured ΔHiin the circumstances should include the changes in the three fractions: adsorption, dehydration and molecular conformation.
It was found that when the unfolded Lys molecules are adsorbed and refold on the surface, entropy-driving caused by the dehydration
of Lys mainly dominates the foregoing process. The refolding enthalpies of Lys, ΔΔHiwere found to be 10~100 folds higher than that measured in usual solutions.</o:p>
Authors:J. Wang, B. Bao, M. Wu, X. Sun, X. Zhang, J. Hu, and G. Ye
This paper reports on the qualitative and quantitative analyses of light hydrocarbons produced by radiation degradation of
N,N-diethylhydroxylamine. The results show that when the absorbed doses are between 10 and 1000 kGy, the main light hydrocarbons
are methane, ethane, ethene, propane and n-butane. Their volume fractions are increased with the increase of the dose. The
volume fraction of ethene is also increased at low doses with the increase of the dose, but it is decreased with the increase
of dose at high doses.
Authors:J. Wang, M. Wu, B. Bao, Zh. Li, Q. Wang, X. Zhang, and G. Ye
This paper reports the study of hydrogen and carbon monoxide produced by radiation degradation of N, N-dimethylhydroxylamine
(DMHA). The results show that when the concentration of DMHA is between 0.1M–0.5M and the dose is between 10–1000 kGy, the
volume fraction of hydrogen is very high and increases with the dose. The volume fraction of hydrogen is little dependent
on the concentration of DMHA at lower dose but increases with increasing concentration of DMHA at higher dose. The volume
fraction of carbon monoxide is very low.
Authors:W. Zhang, K. Ungar, J. Chen, N. St-Amant, and B. Tracy
A quantitative method to determine the activity concentration of 226Ra in soil samples was established using high performance environmental gamma-ray spectrometry. In this method, a semi-empirical
calibration procedure was developed for full energy peak efficiency calculation utilizing the elemental composition of the
soil sample. Aatami software was used to deconvolute the 235U and 226Ra doublet at 185.7 keV and 186.2 keV, respectively, and to fit the baseline of the soil gamma-spectrum for the determination
of 226Ra activity. The results indicated that the Aatami doublet deconvolution procedure provides a rapid and accurate analysis
of a complicated spectrum in comparison with other cumbersome spectral interference correction methods. The study also compared
the results with those obtained by radon progeny (214Pb, or 214Bi) measurements and found that the deconvolution method provided a more accurate 226Ra activity as it is independent of the error caused by radon diffusion. This error can be quite large since the amount of
escaped radon gas through the sample container walls and sealing cannot be accurately quantified.
Authors:Y. Zhang, K. Ma, M. Anand, W. Ye, and B. Fu
Alpha, beta, and gamma diversity are three fundamental biodiversity components in ecology, but most studies focus only on the scale issues of the alpha or gamma diversity component. The beta diversity component, which incorporates both alpha and gamma diversity components, is ideal for studying scale issues of diversity. We explore the scale dependency of beta diversity and scale relationship, both theoretically as well as by application to actual data sets. Our results showed that a power law exists for beta diversity-area (spatial grain or spatial extent) relationships, and that the parameters of the power law are dependent on the grain and extent for which the data are defined. Coarse grain size generates a steeper slope (scaling exponent z) with lower values of intercept (c), while a larger extent results in a reverse trend in both parameters. We also found that, for a given grain (with varying extent) or a given extent (with varying grain) the two parameters are themselves related by power laws. These findings are important because they are the first to simultaneously relate the various components of scale and diversity in a unified manner.
Authors:J. Tian, S. Han, H. Zeng, B. Wang, Z. Li, and Y. Zhang
Microcalorimetric measurements of the polymerization of actin in the presence of 100 mM KCl and 2 mM MgCl2were carried out with a Calvet MS-80 microcalorimeter at temperatures from 293.15 to 310.15 K. It was observed that the polymerization
of actin was endothermic and the enthalpy change for actin polymerization was temperature-dependent. The enthalpy change ΔHowas fitted to ΔHo(kJ mol-1)=434.0-1.16 (T/K) and the change in heat capacity ΔCpocalculated from ΔHowas -1.16 kJ (mol K)-1in the above range of temperatures. The direct calorimetry results showed that the enthalpy and entropy change for actin polymerization
could not be obtained from measurements of the critical concentration and the only way to assess the enthalpy change for the
polymerization of actin and similar reactions lies in the use of calorimetry.
Authors:Y. Li, K. Zhou, M. Jiang, B. Zhang, M. Aslam, and H. Zou
Numerous studies showed that lipid transfer proteins (LTPs) play important roles in flower, development, cuticular wax deposition and pathogen responses; however, their roles in abiotic stresses are relatively less reported. This study characterized the function of a maize LTP gene (ZmLTP3) during drought stress. ZmLTP3 gene was transferred into maize inbred line Jing2416; subsequently the glyphosate and drought tolerance of the over-expression (OE) lines were analyzed. Analysis showed that OE lines could significantly enhance drought tolerance. Transgenic maize lines OE6, OE7 and OE8 showed lower cell membrane damage, higher chlorophyll contents, higher protective enzymes activities, better growth and development under drought condition. The results strongly indicated that overexpression of ZmLTP3 could increase drought tolerances in maize.
Authors:H. Wang, D. Cao, W. Chen, D. Liu, B. Liu, and H. Zhang
Wheat kernel morphology is a very important trait for wheat yield improvement. This is the first report of association analysis of kernel morphology traits in wheat breeding lines. In Qinghai, China, the research described here involved genome-wide association analysis in breeding lines derived from synthetic hexaploid wheat with a mixed linear model to identify the quantitative trait loci (QTLs) related to kernel morphology. The 8033 effective Diversity Array Technology (DArT) markers produced a genetic map of 5901.84 cM with an average density of 1.36 markers/cM. Population structure analysis classified 507 breeding lines into three groups by Bayesian structure analysis using unlinked markers. Linkage disequilibrium decay was observed with a map coverage of 2.78 cM. Marker-trait association analysis showed that 15 DArT markers for kernel morphology were detected, located on nine chromosomes, and explained 2.6%–4.0% of the phenotypic variation of kernel area (KA), kernel width (KW), kernel length (KL) and thousand-kernel weight (TKW). The marker 1139297 was related to both the KL and KA traits. Only six DArT markers were close to known QTLs. The parent SHW-L1 carried eight favored alleles, while other seven favored alleles were derived from elite common wheat cultivars. These QTLs, identified in elite breeding lines, should help us understand the kernel morphology trait better, and to provide germplasm for breeding new wheat cultivars for Qinghai Province or other regions.
Authors:J.-J. Zhang, R.-F. Wang, J.-B. Li, H.-M. Liu, and H.-F. Yang
The thermal decomposition of Eu2(BA)6(bipy)2 (BA=C2H5N–2, benzoate; bipy=C10H8N2, 2,2'-bipyridine)and its kinetics were studied under the non-isothermal condition by TG-DTG, IR and SEM methods. The kinetic
parameters were obtained from analysis of the TG-DTG curves by the Achar method, the Madhusudanan-Krishnan-Ninan (MKN) method,
the Ozawa method and the Kissinger method. The most probable mechanism function was suggested by comparing the kinetic parameters.
The kinetic equation for the first stage can be expressed as: dα/dt=Aexp(–E/RT)3(1–α)2/3.
Authors:X.R. Yu, L. Zhou, J. Zhang, H. Yu, D.R. Gao, B.Q. Zhang, F. Xiong, Y.J. Gu, and Z. Wang
This study was conducted to compare structural development and biochemical accumulation of waxy and non-waxy wheat (NW) caryopses. The caryopses’ microstructure of the waxy wheat (WW) and NW cultivars at different developmental stages were observed under light, fluorescence, and scanning electron microscope. The results were as follows: Compared with NW,WWhad a shorter maturation duration, which was reflected in several following characteristics. Programmed cell death of the pericarp began earlier, and the chlorophyll-containing layer in the pericarp was smaller. Vacuoles in chalazal cells accumulated more tannins at different developmental stages. Starch granules and protein bodies in the endosperm showed a higher accumulation level in developing caryopses, and aleurone cells were larger in size with larger numbers of aleurone grains. An analysis of the element content indicated that the mineral elements Mg, P, K, and Ca exhibited a higher content, while the heavy elements Cr, Cd, and Pb exhibited a lower content in the aleurone layer.