Authors:M.Y. Wang, Y.L. Song, S.X. Zhang, X.L. Zhao, J.W. Wang, N. Niu, and G.S. Zhang
Physiological male sterility induced by the chemical hybridizing agent (CHA) overcomes problems of maintenance of sterile lines and restorers. However, the mechanism of sterility is unclear. The process of tapetum of CHA-treated ‘Xi’nong 2611’ at uninucleate, binucleate and trinucleate were compared with control to determine if tapetum varying differently during developmental stages. Tapetal degradation in CHA-treated ‘Xi’nong 2611’ began at late uninucleate stage, somewhat earlier than control plants. Cytological observations indicated that the gradual degradation of the tapetum in CHA-treated ‘Xi’nong 2611’ was initiated and terminated earlier than in the control. These findings implied that CHA-induced male sterility was related to abnormally early tapetal degradation. In order to indicate the role of the SKP1 gene in fertility/sterility in wheat, its expression was assessed in anthers at uninucleate, binucleate and trinucleate stages. SKP1 expression was reduced in the later developmental stages, and there was an obvious decrease from the uninucleate to trinucleate stages. Higher expression of the SKP1 gene occurred in ‘Xi’nong 2611’ compared to CHA-treated ‘Xi’nong 2611’. This implied that SKP1 gene expression was inhibited during the fertility transformation process and was related to transformation from fertility to sterility. Moreover, the results from this study suggest that SKP1 plays an essential role of conducting fertility in physiological male sterility.
Authors:R.D. Wang, Y.J. Deng, L.J. Sun, Y.L. Wang, Z.J. Fang, D.F. Sun, Q. Deng, and R. Gooneratne
Growth and haemolytic activity of several pathogenic Vibrio species were compared in egg-fried-rice with different egg ratios. Egg-fried-rice preparations with rice-to-egg ratios of 4:1, 1:1, and 1:4 were inoculated with either Vibrio parahaemolyticus, V. cholerae, V. vulnificus, or V. alginolyticus and incubated for 24 h. Cell number, thermostable direct haemolysin (TDH) activity, and total haemolytic activity were determined. The cell number and total haemolytic activity increased in all Vibrio strains after 24 h, and these were most marked in egg-fried-rice with the highest egg content (1:4 (rice:egg) ratio; P<0.05). V. alginolyticus exhibited the maximal growth and V. parahaemolyticus the highest haemolytic activity, but only V. parahaemolyticus ATCC 33847, V. alginolyticus CAMT 21162, and V. alginolyticus HY 91101 showed TDH activity. Results suggest that lowering egg content in egg-fried-rice could reduce growth and virulence of Vibrio pathogens.
Authors:L.L. Han, W.G. Xu, L. Hu, Y. Li, X.L. Qi, J.H. Zhang, H.F. Zhang, and Y.X. Wang
To explore the physiological characteristics of the pepc gene in transgenic wheat (Triticum aestivum) plants, PEPC activities in various organs of T3 plants were analyzed at Feekes 6.0, Feekes 10.3 and Feekes 11.1, and compared to control, untransformed wheat cultivar Zhoumai 19. Net photosynthetic rates (Pn) in leaves were also measured at the same stages. At Feekes 11.1, both transgenic and control plants were treated with DCDP. Yield traits were surveyed after harvest. The results indicated that Pn and PEPC activity in the flag leaf of transgenic wheat were significantly higher than those of the control at different stages. At Feekes 10.3, Pn reached the highest value at 28.2 μmol m−2 s−1 and PEPC activity reached the highest value at 104.6 μmol h−1 mg−1. Both factors significantly increased by 21% compared to the control at Feekes 11.1. PEPC activity in the flag leaf of transgenic plants was significantly higher than that of non-leaf organs. Pn of transgenic plants was greatly reduced after DCDP treatment. In the flag leaf of transgenic wheat, Pn was significantly correlated to PEPC activities at 0.01 probability level with a correlation coefficient of 0.8957**. The yield traits of transgenic line 1-27-3, such as 1000-grain weight, single spike weight and harvest index were higher than those of the control. Additionally, the spike weight of 1-27-3 showed an increase of approximately 9.5% compared to the control. These results indicated that the expression of maize (Zea mays) pepc gene was different across various organs of transgenic wheat and across every growth stage. Therefore, we conclude that introducing maize pepc gene into wheat plants can increase their Pn and improve production.
Authors:X.L. Qi, X.F. Li, F. He, L.Q. Hu, Y.G. Bao, J.R. Gao, and H.G. Wang
Thinopyrum intermedium, which has many useful traits, is valuable for wheat breeding. A new wheat-Thinopyrum addition line, SN100109, was developed from the progeny of common wheat cultivar Yannong 15 and Th. intermedium. It was resistant to most races of Blumeria graminis f. sp tritici (Bgt), which caused powdery mildew in wheat, and its reactions were different from the reactions of gene Pm40 and Pm43. Genomic in situ hybridization (GISH) and molecular marker analysis were used to identify the genomic composition of SN100109. GISH results showed that SN100109 was a wheat-Th. intermedium disomic addition line containing one pair of J chromosomes, and the resistance gene was located on the alien additional chromosomes of SN100109. And four molecular markers BE425942, BF482714, Xgdm93 and BV679214 which were assigned to homologous group 2, were specific molecular markers of the additional chromosomes. All the results indicated that SN100109 contained one pair of 2J chromosomes. SN100109 can be used as a novel germplasm source for introducing powdery mildew resistance genes to wheat in breeding programs.
Authors:S. A. Malik, L. Wang, M. A. Paget, F. Biddlestone, and G. F. Fernando
The authors have previously reported on an optical technique to enable the simultaneous and non-contact acquisition of spectral, thermal and physical information of a sample in a differential scanning calorimeter (DSC). This was achieved using a simple bifurcated fibre optic probe to link the DSC to a conventional Fourier transform infrared spectrometer and an optical spectrum analyser. The fibre optic probe was located over the sample and reference compartments of the DSC. In the current study, a series of experiments were designed to investigate the stability of DSC pans during heating from ambient to 230 °C. During the first heating cycle, the base of the aluminium pans used in these experiments was found to deform in a non-linear manner. The deformation characteristics of pans manufactured from copper and steel were also investigated. Annealing the aluminium pans was found to improve significantly the deformation or expansion characteristics.
Authors:Y. Li, Y. Wang, C. Liu, J. Zhang, L. Jian, and C. He
Systematically complicated technique used for preparing high-intensity (more than 8.0 GBq/cm2)241Am -source by a new enamel technique is presented. High intensity241Am -sources with activities ranging from 3.7 to 37 GBq have been made by this new technique. The activity and photon output have been measured. The results were compared with the data reported by the Radiochemical Centre Amersham in their specification. The photon output of241Am -source produced by us meets the technical specification of241Am -sources produced by Amersham. Moreover, the highest intensity can reach 1789 mCi/cm2. The overall utilization ratio of241Am activity (59.5 keV) is 31.2%.
Authors:B. Xue, X. Li, J. Wang, S. Yu, Z. Tan, and L. Sun
The heat capacities of trans-(R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylic acid in the temperature range from 78 to 389 K were measured
with a precise automatic adiabatic calorimeter. The sample was prepared with the purity of 0.9874 mole fraction. A solid-liquid
fusion phase transition was observed in the experimental temperature range. The melting point, Tm, enthalpy and entropy of fusion, ΔfusHm, ΔfusSm, were determined to be 344.75±0.02 K, 13.75±0.07 kJ mol−1, 39.88±0.21 J K−1 mol−1, respectively. The thermodynamic functions of the sample, H(T)-H(298.15), S(T)-S(298.15) and G(T)-G(298.15), were reported with a temperature interval of 5 K. The thermal decomposition of the sample was studied by TG analysis, the
thermal decomposition starts at ca. 421 K and terminates at ca. 535 K, the maximum decomposition rate was obtained at 525
K. The order of reaction, pre-exponential factor and activation energy, are n=0.14, A=1.15·108 min−1, E=66.27 kJ mol−1, respectively.
Authors:X.-L. Zhou, Y. Yang, Z.-F. Li, B.-H. Wang, and Y.-M. Zhang
The effects of cisplatin and its trans isomer transplatin on the thermal denaturation of G-actin were studied with a Micro DSC-III differential scanning calorimeter. The denaturation enthalpy of G-actin was found to be 12 J g–1, and the denaturation temperature was 328 K. The thermal denaturation curve showed that increasing cisplatin concentration decreased the enthalpy change. However, after the ratio of cisplatin to G-actin attained 8:1 (mol:mol), the denaturation enthalpy no longer decreased. Transplatin decreased the enthalpy change more rapidly. In contrast with cisplatin, the denaturation peak at 328 K disappeared, and a strong exothermic peak appeared at 341 K when the ratio of transplatin to G-actin was 8:1 (mol:mol). The enthalpy change was 75 J g–1, which is far in excess of the range of weak interactions. This strong exothermic phenomenon probably reflects the agglutination of protein. The effects of cisplatin and transplatin on the number of the free thiol groups of G-actin are discussed.
Authors:Chien C. Lin, C. F. Wang, Y.C. Sun, J. H. Chao, and C. L. Tseng
experiments have been carried out to investigate the effects of gamma-radiation
on iodine aerosols under various chemical conditions. The results indicate that
iodide ions (I-) in aerosol can be readily oxidized to I2
and HIO, and some iodide ions may be converted to organic iodine when organic
additives are present in the KI solution from which the aerosol is generated.
The results also suggest that the chemical transformation of irradiated iodine
aerosol depends on the chemical environment both carrier gas and iodide
Authors:W. Wang, S. Luan, Y. Chen, L. Cal, Y. Jia, S. Ruan, and J. Duan
New complexes of the non-natural amino acid (p-iodo-phenylalanine) with divalent cobalt and nickel ions have been synthesized. The composition of the complexes is [M(IC6H4CH2CHNH2COO)2]2.5H2O (M=Co, Ni) and the crystal structure belongs to orthorhombic system. Infrared spectra indicate the nature of bonding in the
complex. The first stage in the thermal decomposition process of the complex shows the presence of crystal water. The thermal
decomposition process of cobalt complex differs from that of nickel. The intermediate and final residues in the thermal decomposition
process have been analyzed to check the pyrolysis reactions. Thermal analysis indicates that the iodine atom of the ligand
may coordinate to the metal ion in the lattice.