Authors:A. Zok, I. Forgács, A. Pedryc, R. Oláh, and E. Szegedi
The biotic and abiotic stresses are the major limiting factors in plant productivity. To overcome these difficulties molecular breeding methods have recently been widely used to improve the stress and disease resistance of grapevine cultivars. Crown gall disease caused by Agrobacterium vitis or Agrobacterium tumefaciens causes serious damage worldwide on grapevine, and there is no efficient method yet that can be routinely used by grape-growers to prevent this disease. Therefore genetic manipulation for crown gall resistance would have a great economic impact. To this end embryogenic culture of Vitis berlandieri × Vitis rupestris cv. Richter 110 was transformed with a virE1 gene construct. Twenty-six plant lines were selected, and their transgenic nature was confirmed by PCR analysis. Seventeen of the 26 lines showed resistance to crown gall disease following inoculation with A. vitis Tm4 strain.
Authors:E. Novák, A. Zok, I. Forgács, A. Pedryc, and R. Oláh
To raise the efficiency of plant regeneration we studied the important and necessary elements of the procedure. The embryogen capacity of 33 various grape genotypes were tested on four different induction media. We successfully obtained anther derived embryogenic calli in 27 genotypes with the range of 1–12%, this is the first reported protocol for embryogenesis from Korai bíbor, Odysseus, Orpheus and Pannon frankos cultivars. Two sorts of sterilization treatments were examined before inducing somatic embryos. For optimisation of grape regeneration system the propagation of calli was attempted in Richter 110 cultivar, there was no any significant differences in the measured values, but CPE medium proved more successful in maintaining embryogenic capacity of callus. We experienced high developmental differences between the propagated embryogenic culture of Richter 110, Teleki 5C and Chardonnay derived from MSNOA liquid medium and from MSE solid medium. Regenerated plants from embryogenic callus were obtained in 21 genotypes, in Chardonnay cultivar CP medium influenced more positively the plant regeneration than the MS/2 medium.