Authors:Huba Kalász, József Lengyel, Tibor Szarvas, György Morovján, and Imre Klebovich
Thin-layer chromatography has been used to monitor formaldehyde production during in-vivo metabolism of (−)-deprenyl. Formaldehyde was reacted with dimedone to yield its dimedone adduct, formaldemedone. Determination of
C-labeled formaldemedone was performed by planar displacement chromatography. The separated bands were detected either by digital autoradiography or on X-ray film after contact autoradiography. Two-dimensional separations have improved the evaluation.