Authors:E. Csányi, K. Bélafi-Bakó, N. Nemestóthy, and L. Gubicza
Ethanol recovery from aqueous fermentation broth by extraction using oleic acid with simultaneous esterification by lipase enzyme was studied. To determine the optimal conditions for the complex process, the ternary system was characterised; binodal curves and tie lines of (ethanol+oleic acid+water) system were determined. Enzymatic esterification of ethanol and oleic acid was carried out and resulted in higher than 50% conversion with simultaneous reduction of ethanol content in the broth. Finally, the effect of the ester product (ethyl oleate) on the distribution of ethanol was determined.