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  • Author or Editor: S. Laskar x
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Abstract  

The present study has been performed to preconcentrate gold using the proteins extracted from Mimusops elengi Linn. (Family: Sapotaceae) seed by radiometric technique using 198Au. Effects of buffer and pH dependence on the binding affinity of the gold have also been examined. It has been found that the binding of gold with M. elengi protein neither depends on the addition of buffer nor the composition (phosphate/citrate) of buffer. The adsorption of gold is also independent of pH of the solution. To verify the gold-protein interaction, inter-comparisons have been made between four different approaches, (1) notably extraction with anion-exchange resin Amberlite IRA 400, (2) trichloroacetic acid (TCA) precipitation, (3) isoelectric precipitation and (4) dialysis of protein after incubation with gold. Good agreement has been observed for all the cases. Binding of gold have been studied with three different concentrations of gold, 1, 10 and 50 ppm spiked with 198Au.

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Abstract  

The tracer packet technique is an efficient tool to evaluate simultaneously the behavior of some closely associated elements. The present study has been performed to determine the binding affinity of various toxic heavy and micronutrient elements with the proteins extracted from Erythrina variegata Linn. (Family: Leguminosae) seed by means of ‘tracer packet’ technique. Effects of buffer and pH dependence on the binding affinity of the elements of interest have also been examined.

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Abstract  

Binding ability of mercury, thallium, lead and bismuth with Erythrina variegata seed protein have been investigated using tracer packet technique. Due to the lack of standard methods, inter-comparisons have been made among three different approaches, like trichloroacetic acid (TCA) precipitation, isoelectric precipitation and dialysis of protein after incubation with the metals. Good agreement was observed for all the cases except that of lead.

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Abstract  

Pleurotus citrinopileatus, a species of edible mushrooms, is widely accepted food component, especially in Indian subcontinent. The accumulating susceptibility of this edible mushroom species towards long-lived radioisotopes of cesium was studied in controlled laboratory condition using the 134Cs (T 1/2 = 2.06 y) radioisotope. It was observed that the experimental mushroom species accumulated 134Cs and maximum accumulation took place in the cap portion. The pileus (cap)/stipes (stem) ratio of each 134Cs accumulated mushroom sample was determined and found 2.22±0.74. The protein and fat fractions of the experimental mushroom species were extracted separately after accumulation of radiocesium and it was found that most of the radiocesium accumulation occurred in the protein fraction of the mushroom. The mushroom Pleurotus citrinopileatus which is white in color, turned completely black after radiocesium accumulation. The black mushroom so obtained was produced upto fourth generation by tissue culture method without using any radiocesium further. All the successors were found to be black indicating a permanent mutation of the mushroom species.

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Summary  

The bioaccumulation of 198Au radionuclide, by Rhizoclonium riparium a member of Chlorophyceae has been studied. It has been observed that accumulation of gold on Rhizoclonium is almost pH independent and slightly higher at basic pH. Accumulation of gold was studied with 198Au radiotracer, 0.1, 1 and 5 ppm concentrations of gold. It has been concluded from the biochemical analysis that the gold accumulation is due to adsorption in the cellulose and not in protein, fat and carbohydrate. Accumulated gold was recovered when washed with conc. HNO3.

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