Lycopene content (LC) and soluble solid content (SSC) are important quality indicators for cherry tomatoes. This study attempted simultaneous analysis of inner quality of cherry tomato by Electronic nose (E-nose) using multivariate analysis. E-nose was used for data acquisition, the response signals were regressed by multiple linear regression (MLR) and partial least square regression (PLS) to build predictive models. The performances of the predictive models were tested according to root mean square and correlation coefficient (R2) in the training set and prediction set. The results showed that MLR models were superior to PLS model, with higher value of R2 and lower values of for RMSE firmness, pH, SSC, and LC. Together with MLR, E-nose could be used to obtain firmness, pH, soluble solid and lycopene contents in cherry tomatoes.
Authors:S.F. Wu, W. Qi, Y.F. Wang, R.X. Su, and Z.M. He
Casein peptides with calcium-chelating capacity were rapidly enriched by using a novel ceramic matrix (CM)-based Ti4+-IMAC adsorbent. The ability of calcium-chelating peptides (CCPs) to bind calcium and the physical properties of complexes formed between CCPs and calcium were investigated. Results demonstrated that the amount of calcium bound depended on the degree of hydrolysis (DH) of casein hydrolysates. The highest calcium binding capacity (683 mg g−1) occurred when bovine casein was hydrolysed by pancreatin at a DH of 0.14%, meanwhile, the calcium content of CCPs-Ca complex exhibited the maximum level (134.96 mg g−1). In addition, CCPs showed a higher radical scavenging capacity (50 µg ml−1; 99% inhibition, or an equivalent activity of 9.91×10−3 M Trolox) compared to casein digest. Moreover, Fourier-transform infrared spectroscopy and fluorescence spectroscopy were used to explore the interaction between CPPs and calcium, and the results demonstrated that phosphoserine residues as well as COO- groups of CCPs were involved in the formation of CCPs-Ca complex.
Authors:W.M. Du, F.H. Wang, H.Y. Zhang, B.Z. Jiang, X.Y. Chen, W. Zhang, Y. Xie, and Z.T. Sheng
Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confirmed that the growth of both beneficial microbe B. longum subsp. longum (0.695, 0.205: R2, slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R2, slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, –0.165: R2, slope, respectively) growth. In vivo studies also revealed that genome copies of Bifidobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment.