An adiabatic calorimeter in which automation of the control of the adiabatic condition and the thermogram recording is achieved
in a simple way has been designed for studies of both thermochemistry and thermokinetics. A new method for specific heat measurements
has been proposed and specific heats ofn-heptane were measured to test the reliability of this calorimeter.
The transformation equation for the thermokinetics of consecutive first-order reactions has been deduced, and a thermokinetic
research method of irreversible consecutive first-order reactions, which can be used to determine the rate constants of two
steps simultaneously, is proposed. The method was validated and its theoretical basis was verified by the experimental results.
Authors:Z. Deng, J. Tian, J. Chen, C. Sun, Y. Zhang, and Y. Wang
wheat is unique to China and has been improved by introducing good germplasm. In order to clear the subunits background of
wheat, sodium dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) was used to detect the high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) compositions in BC1F1, F2 and F3 generations from
wheat. Twenty-five alleles and 49 HMW-GS compositions at the
loci were detected in different generations. Null and subunit 1 were mainly existed at
, and 7 + 8 and 7 + 9 were primarily detected at
in different generations. Subunit combination 5 + 10 mainly appeared in BC1F1, while 2 + 12 major presented in F2 and F3 generations. HMW-GS compositions null, 7 + 8, 5 + 10 and null, 7 + 9, 5 + 10 showed higher frequencies than other banding patterns, followed by null, 14 + 15, 5 + 10 and null, 7 + 9, 2 + 12 combinations. In addition, some rare subunit combinations such as 14 + 15, 13 + 16, 17 + 18, 4 + 12, 2 + 10 and 5 + 12 were found in different generations. Eighteen alleles and 51 LMW-GS compositions at
loci were found in different generations.
d showed higher frequencies than others among three generations. There were mainly a, b, c alleles at
. Thirty, 31 and 14 different combinations were detected in BC1F1, F2 and F3 populations, respectively. There were some good combinations such as
a for different quality characteristics. So some desirable subunit combinations could be selected from different generations and new cultivars with good quality under distinct subunits background should be bred from
wheat in future.
Authors:R.D. Wang, Y.J. Deng, L.J. Sun, Y.L. Wang, Z.J. Fang, D.F. Sun, Q. Deng, and R. Gooneratne
Growth and haemolytic activity of several pathogenic Vibrio species were compared in egg-fried-rice with different egg ratios. Egg-fried-rice preparations with rice-to-egg ratios of 4:1, 1:1, and 1:4 were inoculated with either Vibrio parahaemolyticus, V. cholerae, V. vulnificus, or V. alginolyticus and incubated for 24 h. Cell number, thermostable direct haemolysin (TDH) activity, and total haemolytic activity were determined. The cell number and total haemolytic activity increased in all Vibrio strains after 24 h, and these were most marked in egg-fried-rice with the highest egg content (1:4 (rice:egg) ratio; P<0.05). V. alginolyticus exhibited the maximal growth and V. parahaemolyticus the highest haemolytic activity, but only V. parahaemolyticus ATCC 33847, V. alginolyticus CAMT 21162, and V. alginolyticus HY 91101 showed TDH activity. Results suggest that lowering egg content in egg-fried-rice could reduce growth and virulence of Vibrio pathogens.
Authors:C. Wen, Q. Zhang, Y. He, M. Deng, X. Wang, and J. Ma
A sensitive and simple liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for determination of dasatinib in rat plasma using one-step protein precipitation was developed. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with methanol-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 488.2 for dasatinib and m/z 338.7 for the IS. Calibration plots were linear over the range of 10–1000 ng mL−1 for dasatinib in rat plasma. Lower limit of quantification (LLOQ) for dasatinib was 10 ng mL−1. Mean recovery of dasatinib from plasma was in the range 82.2%–93.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 8%. This developed method is successfully used in pharmacokinetic study of dasatinib in rats.
Authors:Sh. Luo, M. Pu, J. Quao, Zh. Liu, Ch. Zhang, P. Zhao, Y. Fu, and H. Deng
Samarium-153-EDTMP (ethylene diamine tetramethylene phosphonate), for its promising biological properties, has been proved as a palliating therapeutic agent for boné cancer in human beings. In this article, we present the results on synthesis and structure analysis of Samarium-153-EDTMP. In a basic medium,153Sm-EDTMP can be readily prepared with a complexing yield not less than 98%, and it is confirmed that the ratio of the ligand to Sm is 11, and the charge of153Sm-EDTMP is negative two.
Authors:J. Wang, C. Chen, H. Yu, J. Sun, B. Li, Y. Li, Y. Gao, W. He, Y. Huang, Z. Chai, Y. Zhao, X. Deng, and H. Sun
The characterization of different sized TiO2 (25 nm, 80 nm, and 155 nm) was carried out by transmission electron microscopy (TEM) and the micro-distributions of TiO2 in the olfactory bulb of mice after nasal inhalation were investigated by microbeam SRXRF mapping techniques. The results
show that TiO2 particles can be translocated to the olfactory bulb through the olfactory nerve system after inhalation. The distributions
of Fe, Cu, and Zn in the olfactory bulb were also studied.
Authors:Z. Deng, S. Hu, F. Chen, J. Chen, X. Zhang, J. Chen, C. Sun, Y. Zhang, and J. Tian
Two hundred and ninety F9 recombinant inbred lines (RILs) derived from the bread wheat cultivar Gaocheng 8901 and the waxy wheat cultivar Nuomai 1 were used in determining the high-molecular-weight glutenin subunit (HMW-GS) and waxy protein subunit combinations and their effects on the dough quality and texture profile analysis (TPA) of cooked Chinese noodles. Seven alleles were detected at Glu-1 loci. There were two alleles found at each of the Wx-A1, Wx-B1 and Wx-D1 loci. Eight allelic combinations were observed for HMW-GS, LMW-GS and waxy proteins, respectively. Both the 1/7+8/5+10 and 1/7+8/5+12 combinations contributed to dough elasticity, and the 1/7+8/5+10 combination also provided better TPA characteristics. Compared to Wx protein, HMW-GS was more important on dough alveogram properties. LMW-GS significantly affected springiness and cohesiveness; HMW-GS mainly affected the hardness; Wx×LMW-GS significantly affected the springiness, cohesiveness and chewiness; HMW-GS×Wx×LMW-GS mainly influenced the springiness and chewiness. But HMW-GS×LMW-GS only affected the spinginess. These indicated the TPA of noodles was significantly affected by the interactions between glutenin and Wx proteins.
Authors:J.R. Li, D.X. Li, L. Li, W.L. Deng, L.S. Ding, H.X. Xu, and Y. Zhou
A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL−1 ng/mL for tanshinone IIA and 100 pg/mL−1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.