Authors:E. Molina-Trinidad, C. Arteaga de Murphy, and H. Jung-Cook
Good manufacturing practices specify that a well-type scintillation NaI(Tl) crystal detector has to be validated in order
to detect radioactivity from any radiopharmaceutical used to obtain radiopharmacokinetic parameters. A 5 cm well-type NaI(Tl)
scintillation detector was coupled to a multi-channel analyzer centered at the 140 keV 99mTc peak with a 20% window. The area represents counts per minute (cpm). All the net cpm were decay corrected. The activity
source was 99mTc-glucarate developed as an imaging agent for acute myocardial infarction. Wistar rats were injected in a tail vein with
0.1 ml (3.7 MBq) of 99mTc-glucarate solution and 13 blood samples were taken. The cpm were the input data for the WINNONLN program which calculates
radiopharmacokinetic parameters. The detector's efficiency for 99mTc was 15.03% and the sensitivity 1.12 kBq/ml in plasma. The response was linear between 0.31-14.3 kBq/ml of 99mTc-glucarate. The maximum assay variation coefficient was 2.79 and recovery of 99mTc-glucarate in plasma was 99.8%±0.2%. LOD was 0.31 kBq and LOQ = 1.12 kBq in plasma samples. 99mTc-glucarate follows a two-compartment model of distribution with Vd of 21.74 ml±2.71 ml; a Vdss of 74.36 ml±12.67 ml; t1/2a0.74 h±0.19 h; t1/2b 18.98 h±4.36 h; AUC = 32.75 mCi/min.ml ± 3.73 mCi/min.ml; MRT = 24.35 h±5.51 h and total clearance 3.05 ml/h±0.35 ml/h. The well-type detector fulfills the quality system requirements
and the radiopharmacokinetic parameters for 99mTc-glucarate in rats are reliable.
The Radiochemistry Department of Rotem Industries produces 131I hard gelatin capsules for the treatment and diagnosis of different thyroid disorders. Each capsule is designated for a specified patient according to the physician's decision that is based on the patient's morbid status. GMP (Good Manufacturing Practices) requirements have been implemented throughout the production process resulting in the accreditation by the Israeli Ministry of Health. A computerized system has been developed and is used as a QC tool throughout the process of 131I capsule production. The request by the physician for capsules of various activities are introduced into the system and the process from production through packaging and external radiation monitoring is computer-controlled. The system prevents the possibility of double orders, out of specification production and enables documentation of data regardless of production date.
on complete and correct citations, reinforcing qualitycontrol through the whole journal publication chain. However, it appears that the 2010 JCR merely tallies up for each journal, all the citations made to papers published in 2008–2009, irrespective
Authors:Christian Mattle, Nico Heigl, Gudrun Abel, Günther Bonn, and Christian Huck
By use of near infrared (NIR) spectroscopy hyphenated to thinlayer chromatography (TLC) for analysis of methoxylated flavones in a phytomedicine we sought to achieve two objectives: first, to establish a method for rapid, qualitative identification of five methoxylated flavones, denoted G1, G2, G3, G4, and G5, in order of their RF values in normal-phase TLC, and, second, to produce a quantitative model for analysis of G4 (3′,4′,5′-trimethoxyflavone), the compound most representative of Primula veris flowers in phytomedicine. To provide appropriate reference analytical data for building the multivariate cluster and partial least-squares regression (PLS) model, TLC was performed on alumina with n-hexane-ethyl acetate 70:30 (v/v) as mobile phase. Forty-four spectra of eleven independent phytomedicine samples were analyzed with five scans to generate a qualitative cluster model based on PCA (principle-components analysis) that enabled differentiation between G1-G5 on the basis of their methoxylation pattern. This PLS model, in the calibration range between 0 and 1000 mg L−1, enabled quantification of G4 with a standard error of cross validation (SECV) ,54.61 mg L−1. The possibility of conducting qualitative and quantitative analysis simultaneously by use of this method revealed NIRS to be an efficient alternative to conventional modes of detection used for analysis of G1-G5, especially in phytomedicines.
Authors:D. Bagatti, M. C. Cantone, A. Giussani, S. Ridone, C. Birattari, M. L. Bonardi, F. Groppi, A. Martinotti, S. Morzenti, M. Gallorini, and E. Rizzio
The nuclear properties of 186gRe make it a useful agent for radionuclide therapy and imaging. The coordination compound [186gRe]Re-HEDP has proved to be a successful bone seeking agent for palliation of metastatic bone pain. Chemical, radiochemical and radionuclidic purity of commercial radiopharmaceutical [186gRe]Re-HEDP have been checked by means by γ- and β-spectrometries, INAA and paper radio-chromatography. The results indicate a good radionuclidic purity, with levels of contamination from the short-lived 188Re well below the required specifications. After injection of the radiopharmaceutical, the radiochemical measurements conducted in vivo, on biological matrices, blood, plasma and urine, have shown that, entering the systemic circulation, 186gRe dissociates from the bis-phosphonate complex as hydrosoluble [186gRe]ReO4-, and the two chemical species follow different biokinetics.
Authors:E. Knust, C. Müller-Platz, and M. Schüller
2-[18F]-nicotinic acid diethylamide was prepared by nucleophilic aromatic substitution in an acetamide melt of the corresponding
chloro-compound and purified by high pressure liquid chromatography. Optimization of the reaction conditions led to a maximum
radiochemical yield of about 50% within less than one half-life of18F. Tissue distribution of 2-[18F]-nicotinic acid diethylamide in various organs of mice showed a very fast accumulation of activity in the brain (mean body
concentration MBC-239%) with a brain to blood ratio of 1.34.
Authors:Cheng Yang, Jia Guan, Jiang-sheng Zhang, and Shao-ping Li
A simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM),
Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii
. Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine-phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform-
-butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 (
) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines.
Authors:Nirmala Sikder, N. Bulakh, A. Sikder, and B. Gandhe
Qualitative and quantitative analysis of the organic components of composite modified double-base (CMDB) propellants have been performed by HPTLC. The method enabled analysis of a five-component mixture in less than 10 min. The relative distribution of the components of the mixture was determined by means of a UV absorbance detector capable of monitoring at several different wavelengths and of providing UV spectra which could be used to assess peak purity. Detection limits were at nanogram levels.
Authors:Amir Jalilian, Seyyedeh Hosseini-Salekdeh, Morteza Mahmoudi, Hassan Yousefnia, A. Majdabadi, and Majid Pouladian
In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a
modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were
characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O4) was characterized by FT-IR. The particle size was shown to be ≈5–10 nm. To ensure biocompatibility, the interaction of these
SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled
nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature
for at least 2 days and were evaluated for their biodistribution in normal rats up to 24 h compared with free Ga3+ cation and [67Ga]-SPION biodistribution. The biodistribution of the tracer among 3 other folate tracers were compared, showing lower liver
uptake and higher blood circulation after 24 h leading to better bioavailability. The bone:muscle, kidney:muscle, lung:muscle,
stomach:muscle ratios were 9.3, 9.32, 7.6 and 5.83 respectively. The developed folate-containing nano-system can be an interesting
folate receptor tracer, capable of better cell membrane permeability while possessing paramagnetic properties for thermotherapy.