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Abstract

Schizonepeta tenuifolia Briq. (ST) has been used as an aromatic exterior-releasing medicine in clinical practice for thousands of years in China. Previous researches have revealed both volatile oil (STVO) and aqueous extract (STAE) from ST showed significant pharmacological activities, such as anti-virus, anti-inflammation, anti-oxidation, and immunoregulation. However, the influence between each other was still unknown. The purpose of this study was to compare the pharmacokinetic profiles of three main flavonoids (luteoloside, apigetrin, and hesperidin) in STAE to illustrate the influence of STVO. A liquid chromatography-tandem mass spectrometry (HPLC-MS) method was established to quantitatively analyze the three absorbed ingredients in the plasma of healthy rats. Biological samples were analyzed on an Agilent Eclipse Plus C18 column (3.0 mm × 150 mm, 3.5 μm) with gradient mobile phase (containing 0.2% formic acid and acetonitrile) at a flow rate of 0.8 mL/min. All the analytes and quercitrin (IS) were investigated with an electrospray ionization source (ESI) using multiple-reaction monitoring (MRM) in negative ionization mode. In addition, this quantitative method showed good linearities (r ≥ 0.9995) and the lower limits of quantification were 0.590–1.19 ng/mL. The intra- and inter-day precisions ranged 3.47–10.45% and 4.29–11.28% for the three analytes. The mean extraction recoveries were in the range of 77.41–109.79% and the average matrix effects were within 83.41–112.67%. The validated method has been fully applied to compare the pharmacokinetic parameters of the three flavonoid glycosides in rat plasma after oral administration of STAE and STAE+STVO. In comparison of luteoloside, apigetrin, and hesperidin in STAE group, it was found that different degree of increasing existed for the time to reach the maximum concentration (T max), elimination half-life time (T 1/2), the area under the concentration curves (AUC0→t and AUC0→∞) and the maximum concentrations (C max) in STAE+STVO group. As can be seen from above results, STVO could improve the absorption and bioavailability of the three analytes. These findings would provide some active and strong basis of safe clinical application for ST and further exploitation for STVO from the perspective of drug–drug interaction.

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. 33 Soldin, S. J., Soldin, O. P.: Steroid hormone analysis by tandem mass spectrometry. Clin. Chem., 2009, 55 (6), 1061–1066. 34 Hsing, A. W

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Orvosi Hetilap
Authors: Béla Gyarmati, Eszter Szabó, Balázs Szalay, Áron Cseh, Noémi Czuczy, Gergely Toldi, Barna Vásárhelyi, and Zoltán Takáts

Murphy, A. T., Witcher, D. R., Luan, P. és mtsa: Quantitation of hepcidin from human and mouse serum using liquid chroma tography tandem mass spectrometry. Blood, 2007, 110 , 1048–1054. Luan P

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Acta Veterinaria Hungarica
Authors: István Szatmári, Péter Laczay, and Zsuzsa Borbély

-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Anal. Chem. 74 , 1509–1518. Nau H. Determination of persistent tetracycline residues in soil fertilized

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. Rosén , J. & Hellenäs , K.-E. ( 2002 ): Analysis of acrylamide in cooked foods by liquid chromatography tandem mass spectrometry . Analyst , 127 , 880 – 882

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, H. , GERMAN , J.B. , KJELDEN , R. , LEBRILLA , C.B. , BARILE , D. ( 2013 ): Quantitative analysis of gangliosides in bovine milk and colostrum-based dairy products by ultra-high performance liquid chromatography-tandem mass spectrometry

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cepa L.) infected by Fusarium oxysporum using liquid chromatography–tandem mass spectrometry . Food Chem. , 133 , 1653 – 1657 . Ly , T.N. , Hazama

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. Elaneeda , Development and validation of fast and simple flow injection analysis-tandem mass spectrometry (FIA-MS/MS) for the determination of metformin in dog serum , J. Pharm. Biomed. Anal. 107 ( 2015 ) 229 – 235 . [15

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(AKH) precursor processing in Locusta migratoria obtained by capillary liquid chromatography-tandem mass spectrometry. Peptides 23 , 635–644. Schoofs L. New insights in

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Acta Microbiologica et Immunologica Hungarica
Authors: Katalin Szakmár, Olivér Reichart, István Szatmári, Orsolya Erdősi, Zsuzsanna Szili, Noémi László, Péter Székely Körmöczy, and Péter Laczay

electrospray ionization tandem mass spectrometry. Anal Chem 74 , 1509–1518 (2002). Nau H Determination of persistent tetracycline residues in soil fertilized with liquid manure by high

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