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Acta Alimentaria
Authors: B. Vecseri-Hegyes, P. Fodor, and Á. Hoschke

The most sensitive and time-consuming technological step of beer production is the fermentation of wort. Problems during fermentation will not only prolong production time but it will lead to the deterioration of beer quality. Most often it is due to low zinc concentration of the wort or the yeast. Due to lack of zinc the fermentation lasts longer, composition of fermentation by-products changes, maltose intake slows down, sedimentation ability and heat sensitivity of the yeast increase. In Part I of our work, the factors affecting the degree of zinc supply of wort were examined. During the examination of zinc supply of wort two kinds of beer were produced: all-malt beer and beer with adjunct. Change of zinc concentration was followed throughout the brewing process at every technological step. It was found that concentration of zinc gradually decreases during production of wort, and only a small fraction of the calculated amount appears in it. Wort made with adjunct has even lower zinc concentration than all-malt wort. In all malt wort 3.4%, while in wort containing adjunt only 0.4% of the zinc appeared in the wort. Yeast can absorb only the ionic form of zinc during fermentation, thus we had elaborated a method for the separation of the organic and inorganic form of zinc, which was followed by the determination of the concentration of ionic zinc in wort prior to fermentation.

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Chelidonii herba has long been known in herbal medicine for its choleretic, cholagogue, spasmolytic and antiviral activities. It is important to monitor the amount of mineral elements in Chelidonii herba preparations consumed mainly for their phytotherapeutical effects. In addition to organic compounds, dissoluble mineral elements in the teas and tinctures of herbs may also have a role in therapy. The concentration of 24 elements (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Ti, V, Zn) in crude drugs (herb and root), in their aqueous solutions (infusion, decoction) and alcoholic extracts were studied by ICP-OES. The difference between the concentration of elements in extracts - except for copper, manganese and sodium - was highly significant. It has been found that the root contains higher concentrations of mineral elements except for boron, copper, phosphorus and sulphur. The infusion contained most elements in the highest concentration and proved to be the best source for obtaining minerals. The same tendency was observed in the case of dissolutions. In aqueous extracts, the dissolution of mineral elements was between 10% and 65% for most elements, especially for potassium (65%) and phosphorus (54%). The dissolution of mineral elements in the case of tinctures decreased with increasing alcohol concentration. It may be stated that the presence of macro- and microelements in extracts greatly contribute to their therapeutical value.

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Antifungal effect of Micromeria myrtifolia Boiss. & Hohen. in Boiss. and Prangos uechtritzii Boiss. Hawsskn decoctions was tested against Alternaria alternata, Aspergillus niger, Aspergillus parasiticus, Botrytis cinerea, Fusarium oxysporum f.sp. melonis and Penicillium digitatum. Of the 2 substances tested Pr. uechtritzii, being present at 75 to 80% concentration in potato dextrose agar, partly inhibited growth of A. alternata, B. cinerea and P. digitatum. Pr. uechtritzii had higher antifungal effect than M. myrtifolia on mycelial growth during incubation. M. myrtifolia partly affected mycelial growth of A. alternata and A. niger at the beginning of incubation. But the mycelial growth of F. oxysporum was not inhibited by M. myrtifolia concentrations during incubation. Also, Pr. uechtritzii did not have any affect on mycelial growth of A. niger during incubation P. digitatum, the most sensitive microorganism to both decoctions. Higher decoction concentrations of plants used in study will be probably inhibit mycelial growth of microorganisms.

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The production of Kluyveromyces marxianus biomass enriched with copper ions were studied. For that reason the growth of Kluyveromyces marxianus in whey with different concentrations of copper ions in batch process under semiaerobic and aerobic conditions were examined. The kinetics of copper ions accumulation in yeast cells, under the same conditions, as well as the reduction of chemical oxygen demand (COD) during yeast growth in aerobic condition, were monitored, as well. The concentration of copper ions in media up to 4 mg l −1 did not affect the yeast growth, whereas at a higher concentration, a marked decrease in the rate of yeast growth, ethanol production and lactose consumption occurred. In semiaerobic conditions, the maximum uptake of 0.35 mg Cu g −1 d.m. was obtained, while in aerobic conditions a lower uptake of 0.26 mg Cu g −1 d.m. and COD reduction of 85% were achieved. COD reduction was independent of the copper addition or uptake.

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Abstract

This paper is about comparing the effectiveness of the sorbent materials of granulated activated carbon in removing of bromates from drinking water. The limit value (10 µgl−1) of bromates in the drinking water was stated by World Health Organization. In order to insure the reduced concentration of bromates in the drinking water, an experiment was performed using the batch test. In this experiment different types of sorbent materials with different properties were testing. Based on batch test were measured the parameters like the immediate adsorption capacity, the adsorption efficiency, and the concentration of bromates after the adsorption were measured at the evaluated time.

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A capillary isotachophoretic (cITP) method to determine the concentration of nitrates and nitrites in vegetables and lactic acid fermented tomato, cucumber and cucumber-onion juices is described. Method characteristics (selectivity, response function, linearity, precision, accuracy and limit of quantification) were determined. The minimal sample pretreatment and relatively low running make isotachophoresis a good alternative to existing methods.

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In this study honey samples of Eucalyptus honey (Eucalyptus lanceolatus) were botanically characterized. Response surface methodology was used to analyze the effect of temperature, time and pH on the quality responses (hydroxymethylfurfural concentration and diastatic activity) of Eucalyptus honey. A central-composite rotatable design was used to develop models for the responses. At the central value of time (10 min), the maximum concentration of hydroxymethylfurfural was demonstrated at the highest temperature and pH. The maximum value of hydroxymethylfurfural concentration was also obtained at the maximum time and temperature, while keeping the pH at the central value of 5.3. Diastatic activity decreased as the pH moved away from the central pH value of 5.3 to 5.6 at any level of temperature and time. Three-dimensional response surfaces were superimposed, and the overlapping regions gave the diastatic activity (calculated as diastase number 9 to 23.09 °G) and hydroxymethylfurfural concentration (3 to 10.21 mg kg-1) at 48±1 °C for 9.5±1 min at 5.15±0.15 pH.

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Abstract

The main goal of this study is firstly to model the phenomenon of advection and diffusion of the gaseous residues of coal combustion. On the other hand, to study the dispersion of this pollution by varying the parameters like the wind and the resources of pollution, where there are four emissions: SO2, CO, NO2 and PM10. The present model will compare estimating concentrations with the results found by the contribution of the thermal power plant of Jerada city, where the American Environmental Regulatory Model is used. The results suggest that SO2, PM10, NO2 and CO concentration simulated by turbulent k-ε model in combination with the transport model of diluted species, at the 3h and 24h intervals, greater than their respective observed concentrations compared with the American Environmental Regulatory Model. The concentrations of the model found are very high and exceed the limit values under the study conditions. Indeed, the simulated model was used in 2D with a very low wind speed (10−4 m/s) and in the absence of gravity, hence the total neglect of altitude because the two sources of pollution are at the height of 120 m.

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Alpha keto acids are important food additives, which commonly produced by microbial deamination of amino acids. In this study, production of phenylpyruvic acid (PPA), which is the alpha keto acid of phenylalanine was enhanced in 2-l bench scale bioreactors by optimizing of fermentation medium composition using the Box-Behnken Response Surface Methodology (RSM). Optimum glucose, yeast extract, and phenylalanine concentrations were determined to be 119.4 g 1−1, 3.7 g 1−1, and 14.8 g 1−1, respectively, for PPA production, and 163.8 g 1−1, 10.8 g 1−1, and 9.8 g 1−1, respectively, for biomass production. Under these optimum conditions, PPA concentration was enhanced to 1349 mg 1−1, which was 28% and 276% higher than the unoptimized bioreactor and shake-flask fermentations, respectively. Moreover, P. vulgaris biomass concentration was optimized at 4.36 g 1−1, which was 34% higher than under the unoptimized bioreactor condition. Overall, this study demonstrated that optimization of the fermentation media improved PPA concentration and biomass production in bench scale bioreactors compared to previous studies in the literature and sets the stage for scale up to industrial production.

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Herbal teas may be effective in adjuvant therapy for the prevention of complications of diabetes mellitus II. Agrimoniae herba, Betulae folium, Bursae pastoris herba, Foenigraeci semen, Galegae herba, Maydis stigma, Taraxaci radix, Phaseoli fructus sine seminibus, Urticae folium, and their teas were analysed by ICP-OES for element content. Concentration of the elements of crude drug samples obtained are in good agreement with the average concentrations of plants, although some significantly high concentration has been found for manganese (893 mg kg-1), zinc (275 mg kg-1) and iron (492 mg kg-1) in Betulae folium, for copper (41.2 mg kg-1) in Galegae herba, for iron (2692 mg kg-1) in Taraxaci radix, for calcium (41210 mg kg-1) and magnesium (6275 mg kg-1) in Urticae folium. The common characteristic feature of crude drugs is the relatively high concentration of chromium (0.8-16.3 mg kg-1). Ion concentrations of teas are relatively low. The quantities of metal ions do not cover the daily needs, nevertheless, they may be important as food supplements. According to U.S. Recommended Dietary Allowances (RDA), the following teas are good sources: Agrimoniae herba for chromium, Betulae folium for manganese, Taraxaci radix for copper and chromium, Urticae folium for potassium and calcium. The dissolution of elements from plant drugs varies: potassium (22.5-74.7%), sodium (1.9-60.5%), calcium (6.6-28.1%), magnesium (12.3-52.5%) and copper (3.7-51.4%) are readily dissolved. The dissolution of manganese (6.8-32.3%) and of zinc (0-31%) is lower. The dissolution of chromium from Agrimoniae herba (9.55%) is significantly higher, while the dissolution ranges from other plant drugs are between 0-5.9%.

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