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Acta Physiologica Hungarica
Authors: Simona Clichici, C. Catoi, T. Mocan, A. Filip, C. Login, A. Nagy, D. Daicoviciu, N. Decea, C. Gherman, R. Moldovan, and Adriana Muresan

Oxidative stress is related to the liver fibrosis, anticipating the hepatic stellate cells’ (HSC) activation. Our aim was to correlate oxidative stress markers with the histological liver alterations in order to identify predictive, noninvasive parameters of fibrosis progression in the evolution of toxic hepatitis.CCl4 in sunflower oil was administered to rats intragastrically, twice a week. After 2, 3, 4 and 8 weeks of treatment, plasma levels of malondialdehyde (MDA), protein carbonyls (PC), hydrogen donor capacity (HD), sulfhydryl groups (SH), and glutathione (GSH) were measured and histological examination of the liver slides was performed. Dynamics of histological disorders was assessed by The Knodell score. Significant elevation of inflammation grade was obtained after the second week of the experiment only (p=0.001), while fibrosis started to become significant (p=0.001) after 1 month of CCl4 administration. Between plasma MDA and liver fibrosis development a good correlation was obtained (r=0.877, p=0.05). Correlation between PC dynamics and liver alterations was marginally significant for inflammation grade (r=0.756, p=0.138). HD evolution revealed a marginally inverse correlation with inflammation grade (r=−0.794, p=0.108). No correlations could be established for other parameters with either inflammation grade or fibrosis stage.Our study shows that MDA elevation offers the best prediction potential for fibrosis, while marginal prediction fiability could be attributed to high levels of plasma PC and low levels of HD.

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Membrane emulsification (ME) is a relatively new technique for the highly controlled production of particulates, which helps to obtain a narrower distribution compared to other emulsification techniques such as homogenizers or ultrasound. Benefits of membrane emulsification for food applications include the low shear properties and the uniform size distribution. In this process, the dispersed phase (oil) is pressed through the pores of a microporous membrane directly into the continuous phase (water) flowing tangentially to the membrane surface. The purpose of the emulsification experimentations was to find and model operating conditions of the operation. In laboratory experiments from conventional, commercial grade sunflower oil (dispersed phase) and from distilled water (solid phase), emulsions were prepared. The ceramic tube membrane with nominal pore size of 1.4 μm was used in the experiments (ZrO2). In order to increase the shear-stress near the membrane wall (influence the characteristics of the flow regime of the continuous phase), a kind of self-fabricated helical-shaped-ribbon reducer was installed inside the tube membrane.

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We evaluated the microbiological safety, the short-term storability, and the macronutrients of oil seed cake (OSC) obtained from walnut (WnC), linseed (LC), and sunflower seed with hull (SC). The OSCs had 3.6–5.8% moisture content, 0.50–0.60 water activity (aw), 29.9–39.4% protein, 15.5–23.6% fat, and 36.6–48.0% dietary fibre content. The grinded OSCs could be stored in opaque plastic bags for 4 months at temperatures of 4 °C, 14 °C, and 25 °C. Total colony count of mesophilic microorganisms depending on oxygen demand and spore-forming ability, and faecal indicator microorganisms were determined during a 4-month storage term at 4 °C, 14 °C, and 25 °C using traditional culturing methods. The OSCs were free from sulphite-reducing Clostridia and coliforms, including Escherichia coli. Data were analyzed statistically by multifactor analysis of variance. Ascending order of the average contamination of the three products (log10 CFU g−1) was aerobic sporogenic bacteria (2.39), fungal count (2.51), total aerobic microbe count (3.00), anerobic sporogenic bacteria (3.75), and total anaerobic microbe count (4.23). As for the average microbial count, WnC was the least (2.73 log10 CFU g−1) and LC was the highest (3.53 log10 CFU g−1) contaminated material. Regarding the variation of microbial contamination during storage, temperature was indifferent (P=0.191), while storage time (P=0.0033) and the product type were influential (P=0.000).

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Acta Alimentaria
Authors: J. Tarek-Tilistyák, J. Agócs, M. Lukács, M. Dobró-Tóth, M. Juhász-Román, Z. Dinya, J. Jekő, and E. Máthé

The nutritive value, the microbiological safety of oilseed cake (OSC) obtained from naked pumpkin seed (PuC), sunflower seed (SC), yellow linseed (LC), and walnut (WnC), and their impact on wheat flour (WF) dough and bread sensory characteristics at 5% and 10% addition ratio were investigated. The OSCs had high protein (34–50%), fat (8–15%), total dietary fibre (23–36%) content and high energy value (383–444 kcal/100 g)). The OSC samples with a minimal exception fulfilled the requirements of feed legislation in force. An increased water absorption, dough development time, and reduced elasticity were observed probably due to the enhanced fiber and protein content. Dough stability increased with WnC, and decreased with PuC or SC addition. Enrichment provided the appearance of a brown bread for WnC, of a half-brown bread for LC. PuC gave an unusual look. The appearance of OSC fortified bread similar to daily bread, was an advantage resulting the 1st rank for 10% WnC bread and the 2nd one for 10% LC bread (P=0.05). The studied OSCs are suitable for food enrichment, however, in case of PuC and SC fortified flour blends, hydrocolloid application is recommended. Our data suggest that the newly developed fortified breads could be a valuable source for healthy nutrition.

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Replacement of animal fat with plant oils is a very popular research field, due to the increasing prevalence of cardiovascular diseases in association with saturated fatty acid consumption. The aim of this study was to reduce amount of pig backfat in a meat product (Párizsi) and to partially replace it with soybean- (SBL) or sunflower lecithin (SFL). Between the samples difference was realized in fatty acid (FA) profile, mostly in total n6 FA content. The replacement also altered the colour compared to the control. The oxidative stability (MDA) analysis showed that SBL was more prone towards preparation technology (10 nmol MDA/g) than the Sfl(9 nmol MDA/g). The lecithin appeared as foreign taste based on the sensory test. The aromatic difference, as assessed with electronic nose, was clearly detectable between Sfland SBL. The increasing supplementation levels were also properly distinguished with discriminant analysis within the SBL and Sflseries. Summarized, Sflwas found to be a better antioxidant, but SBL improved the FA profile into a more favourable state. The lecithin-replacement made unlikeness in the taste compared to the control.

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Acta Alimentaria
Authors: K. Szentmihályi, A. Blázovics, I. Kocsis, Edit Fehér, B. Lakatos, and Péter Vinkler

The metabolic alteration of fatty liver causes significant changes in the composition of bile fluid secreted by epithelial liver cells. These processes include both organic molecular and inorganic ion content changes in bile juice as well. In this work ion concentration differences of bile fluid in experimental hyperlipidemic rats compared to normal ones were monitored. Fatty liver in young male Wistar albino rats was induced by 2% cholesterol, 20% sunflower oil and 0.5% cholic acid added to the normal food and 3% alcoholic water was administered to rats for 9 days. The development of hyperlipidemy was detected by measuring serum parameters and fatty liver was proved by morphological investigations. The ion concentrations of bile fluid samples were determined by ICP-OES (inductively coupled plasma optical emission spectrometry). The bile fluid samples were digested with a mixture of HNO3 and H2 O2 . The results show that the concentration of calcium and phosphorus in bile juice increased significantly in hyperlipidemic rats compared to the control. Significant concentration changes have also been detected for chromium and manganese. The changes in metal ion metabolism also point to the damage of liver cells owing to metal complex evacuation.

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Abstract

Water availability is one of the major physiological factors influencing plant growth and development. An assessment study has been done at the Szent István University, Gödöllő to evaluate and identify the water footprint of protein yield of field crop species. Twelve field crop species (Sugar beet Beta vulgaris, spring and winter barley Hordeum vulgare, winter wheat Triticum aestivum, maize Zea mays, sunflower Helianthus annuus, peas Pisum sativum, potato Solanum tuberosum, alfalfa Medicago sativa, oilseed rape Brassica napus, rye Secale cereale and oats Avena sativa) were involved in the study. Evapotranspiration patterns of the crops studied have been identified by the regular agroclimatology methodology and physiologically reliable protein ranges within crop yields were evaluated.

The results obtained suggest, that water footprint of cereals proved to be the lowest, however maize values were highly affected by the high variability of protein yield. Oilseed crops had considerably high protein yield with medium water efficiency. Alfalfa, potato and sugar beet water footprints were in accordance with their evapotranspiration patterns.

Protein based water footprint assessment seems to be more applicable in crop species evaluations than that of yield based methodologies.

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. et al., 1995. Genotypic variation in kernel cadmium concentration in sunflower germplasm under varying soil conditions. Crop Sci. 35 . 137--141. Genotypic variation in kernel cadmium concentration in sunflower germplasm

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Mészlepedékes csernozjom vályogtalajon, az MTA TAKI Nagyhörcsök Kísérleti Telepén vizsgáltuk a K és B elemek közötti kölcsönhatásokat 1988-ban napraforgó jelzőnövénnyel. Az alaptrágyázás 100–100 kg N és P2O5·ha-1 volt. A K-szinteket 0, 1000 és 2000 kg·ha-1 K2O feltöltő adaggal, a B-szinteket 0, 20, 40 és 60 kg·ha-1 adaggal állítottuk be 1987 őszén, lucerna elővetemény után. A műtrágyákat Ca-ammónium-nitrát, szuperfoszfát, 60%-os KCl és 11%-os bórax formában alkalmaztuk. Főparcellaként a 3 K-szint, alparcellaként a 4 B-szint szolgált. A kísérletet 12 kezeléssel 3 ismétlésben, összesen 36 parcellával, osztott parcellás (split-plot) elrendezésben állítottuk be. A termőhely szántott rétege 5% körüli CaCO3-ot, 3% humuszt, 20–22% agyagot tartalmazott. A talaj eredetileg N, Ca, Mg és Mn elemekkel jól, káliummal közepesen, P és Zn elemekkel viszonylag gyengén ellátottnak minősült. A talajvíz 13–15 m mélyen helyezkedik el, a termőhely aszályérzékeny. A napraforgó tenyészideje alatt 290 mm csapadékot kapott (a sokéves átlaghoz közeli érték), igen száraz volt viszont a május és a július. A főbb megállapítások, eredmények: – Betakarítás idején a ha-onkénti tőszám 34 ezerről 23 ezerre csökkent igazolhatóan a B-terhelés nyomán a K-kontroll parcellákon. A tőszámcsökkenés nagyobb tányérokat, ezerkaszattömeget és tányéronkénti kaszattömeget indukálva terméskiegyenlítődést eredményezett. A káliummal feltöltött parcellákon a bór ilyen irányú negatív hatása elmaradt. A kaszat 2,1, a szár szintén 2,1, a tányér 1,3, az összes légszáraz föld feletti biomassza tömege 5,5 t·ha-1 mennyiséget tett ki. – A B-trágyázás igazolhatóan növelte a napraforgó szerveinek B-tartalmát, míg a K-trágyázás igazolhatóan vagy tendencia jelleggel mérsékelte. Az aratáskori tányérban halmozódott fel a legtöbb bór (69 mg·kg-1 átlagos koncentrációban), mely a szár és a kaszat átlagos B-készletét mintegy 3-szorosan múlta felül. A K-trágyázással a növényben mért K-tartalom érdemben nem módosult. – A 4–6 leveles korú gyökérben főként a Na és Fe, a hajtásban a N, K, Ca és Mg, az aratáskori tányérban a B mellett a Ca és Cu, míg a kaszatban a N, P, Zn és Cu elemek dúsultak. A szár N, P, Zn és Cu elemekben szegényedett el, melyekben a generatív kaszat dúsult. – Az 1 t kaszattermés + a hozzá tartozó szár és tányér melléktermés elemtartalma közelítően 46 kg N, 40 kg K (47 kg K2O), 27 kg Ca (38 kg CaO), 7 kg P (16 kg P2O5), 7 kg Mg (11 kg MgO) mennyiséget tett ki. Adataink iránymutatóul szolgálhatnak a napraforgó elemigényének becslésekor a szaktanácsadásban. – A talajzsaroló, illetve trágyaigényesnek tartott napraforgó trágyaigénye drasztikusan lecsökken kombájn betakarításnál, amennyiben a K, Ca és Mg elemek döntően a táblán maradó melléktermésben találhatók és el sem kerülnek az adott tábláról.

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Krička, Tajana; Pliestić, S.; Dobričević, Nadica (1998): The Influence of Incresead Moisture on the Velocity of Drying, Dinamic Properties and Fluidization of Sunflower Seed. Agricultural Scientific Review, Faculty of Agriculture, University of Zagreb

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