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Kaurah, P., MacMillan, A., Boyd, N. és mtsai: Founder and recurrent CDH1 mutations in families with hereditary diffuse gastric cancer. JAMA, 2007, 297 , 2360–2372. Boyd N. Founder and

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Acta Microbiologica et Immunologica Hungarica
Authors: Farnaz Zahedi Avval, Shadi Shomali, Mohammad Nadri, Reza Boostani, Lida Jarahi, and Masoud Youssefi

ST2 is a member of IL-1 receptor family expressed on Th2 cells and regulates Th2 responces. The gene of ST2 encodes soluble ST2 (sST2) and the transmembrane ST2 (ST2L) isoforms through alternative mRNA splicing. The discovery of IL33/ ST2 signaling pathway, has drawn a great scientific attention to this system. sST2 has been shown to be an indacating factor in various infl ammatory conditions. This study aims to evaluate serum sST2 levels in HTLV-1 infected patients. This study included 49 HTLV-1 seropositive cases of which 14 were sympthomatic. Controls consisted of 30 healthy volunteers. sST2 level was measured using a quantitative ELISA assay and the results of the study groups were compared. Corroborating the previous reports, sST2 was lower in females (P = 0.003). The sST2 levels was slightly increased in HTLV-1 patients, though such increase was not statistically significant (P = 0.91), in addition sST2 level did not correlate significantly to the disease duration (P = 0.78). Despite some other chronic viral infection, HTLV-1 seems not to induce high serum sST2. However owing to relatively high normal variation of sST2 levels and rather small sample size, we stongly recommend further reseach with preferably larger sample size to evalute sST2 in HTLV-1 infected patients.

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Cytolethal distending toxins (CDT) represent an emerging toxin family, widely distributed among pathogenic bacteria. The cdtABC genes in E. coli are either part of the genome of prophages, plasmid or pathogenicity island. In order to investigate the stability and the transfer potential of cdt-IV genes cdtB gene was replaced by chloramphenicol (Cm) resistance encoding cat gene in the avian pathogenic E. coli (APEC) strain E250. After consecutive passages in non-selective medium at 37 °C 7.6% (219/2900) of the investigated colonies of E250::cat strain became Cm-sensitive (CmS). To reveal deletion mechanism 177 CmS colonies were investigated for presence of cdtA, cdtC and cdtC associated gene by PCR. One hundred and sixteen colonies of the CmS colonies (65.5%) showed partial or complete deletion in the cdt-IV region. Progressive loss of the upstream genes of the cdt cluster in E250 compared to other CDT-IV producing APEC strains and the fact that all the potential deletion patterns were identified, suggests the presence of an unstable hitherto unknown genomic region. The failure of in vitro transfer of cdt genes into a porcine EPEC E. coli strain suggests that the deletion of cdt-IV flanking genes alone do not promote the spread of cdt-IV.

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Abstract

Chronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhibitor) is found to attenuate the levels of IL6 and Cox 2 which are induced by HBV replication.

The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. Our results show that HBx is involved in the increase of the expression of Cox2 through the NFκB pathway. However, the expression of Cox2 is decreased when the HBx-expressing cells are incubated with ibuprofen. The contrasting effect of HBx on Cox2 is found to be determined by differential dimer formation among the members of the NFκB family of proteins, including NFκB, RelA, and C-rel. Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. Additional information on the interaction network involving HBx, ibuprofen, and NFκB pathways is revealed by two-dimensional liquid chromatography-tandem mass spectrometry proteomics analysis. Taken together, our findings provide new insights on the angiogenesis induced by HBV replication.

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Absztrakt:

A közlemény az orozomukoid és ezen belül a sziálsav molekuláris szerkezetét, strukturális sajátosságát mutatja be, valamint ismerteti élettani, kórélettani és klinikai tulajdonságait, illetve szerepét. Az orozomukoid a lipokalinok családjába, illetve az immunokalinok családjába tartozó, az immunrendszerre ható antiinflammatoricus, valamint transzportmolekula. Az orozomukoid további sajátossága, hogy lektinekhez tud kapcsolódni. Az orozomukoid elterjedt a természetben, és további vizsgálata révén a funkciójával, biológiai szerepével kapcsolatos ismeretek is gazdagodni fognak. A cikkben a klinikai vonatkozások is említést nyernek. Orv Hetil. 2019; 160(8): 283–290.

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Absztrakt

Az utóbbi években megjelent tanulmányok egyre inkább alátámasztják, hogy bár a sclerosis multiplex már fiatal felnőtt korban manifesztálódik, ennek ellenére nem foszthatja meg a reproduktív korú nőket az anyaság csodálatos érzésétől. Sőt – az ellentmondásos nemzetközi irodalom ellenére – védőfaktor magára a betegségre, és nincs káros hatással a magzatra. Az immunmoduláns terápia alkalmazása terhesség alatt azonban egyedi elbírálást és gondos körültekintést kíván, amellyel a szakember hozzájárulhat a sclerosis multiplexben szenvedő édesanya családi boldogságához. Orv. Hetil., 2015, 156(34), 1360–1365.

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Acta Veterinaria Hungarica
Authors: Sándor Hornok, Attila D. Sándor, Gábor Földvári, Angela M. Ionică, Cornelia Silaghi, Nóra Takács, Anna-margarita Schötta, and Michiel Wijnveld

Abstract

Recently, the occurrence of Ixodes (Pholeoixodes) kaiseri has been reported for the first time in several European countries, but data on the molecular analysis of this hard tick species are still lacking. Therefore, in this study DNA extracts of 28 I. kaiseri (collected from dogs and red foxes in Germany, Hungary and Romania) were screened with reverse line blot hybridisation (RLB), PCR and sequencing for the presence of 43 tick-borne pathogens or other members of their families from the categories of Anaplasmataceae, piroplasms, rickettsiae and borreliae. Rickettsia helvetica DNA was detected in one I. kaiseri female (from a red fox, Romania), for the first time in this tick species. Six ticks (from red foxes, Romania) contained the DNA of Babesia vulpes, also for the first time in the case of I. kaiseri. Molecular evidence of R. helvetica and B. vulpes in engorged I. kaiseri does not prove that this tick species is a vector of the above two pathogens, because they might have been taken up by the ticks from the blood of foxes. In addition, one I. kaiseri female (from a dog, Hungary) harboured Babesia sp. badger type-B, identified for the first time in Hungary and Central Europe (i.e. it has been reported previously from Western Europe and China). The latter finding can be explained by either the susceptibility of dogs to Babesia sp. badger type-B, or by transstadial survival of this piroplasm in I. kaiseri.

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Acta Veterinaria Hungarica
Authors: Virág Somogyi, Tamás L. Horváth, István Tóth, Tibor Bartha, László Vilmos Frenyó, Dávid Sándor Kiss, Gergely Jócsák, Annamária Kerti, Frederick Naftolin, and Attila Zsarnovszky

Thyroid hormones (THs) and oestrogens are crucial in the regulation of cerebellar development. TH receptors (TRs) mediate these hormone effects and are regulated by both hormone families. We reported earlier that THs and oestradiol (E2) determine TR levels in cerebellar cell culture. Here we demonstrate the effects of low concentrations (10–10 M) of the endocrine disruptor (ED) bisphenol A (BPA) on the hormonal (THs, E2) regulation of TRα,β in rat cerebellar cell culture. Primary cerebellar cell cultures, glia-containing and glia-destroyed, were treated with BPA or a combination of BPA and E2 and/or THs. Oestrogen receptor and TH receptor mRNA and protein levels were determined by real-time qPCR and Western blot techniques. The results show that BPA alone decreases, while BPA in combination with THs and/or E2 increases TR mRNA expression. In contrast, BPA alone increased receptor protein expressions, but did not further increase them in combination with THs and/or E2. The modulatory effects of BPA were mediated by the glia; however, the degree of changes also depended on the specific hormone ligand used. The results signify the importance of the regulatory mechanisms interposed between transcription and translation and raise the possibility that BPA could act to influence nuclear hormone receptor levels independently of ligand–receptor interaction.

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Orvosi Hetilap
Authors: Zoltán Sohajda, Márta Hódos, and László Módis

Absztrakt:

A Wilson-kór autoszomális recesszív öröklésmenetet mutató, kóros rézfelhalmozódással járó anyagcsere-betegség. Tanulmányunkban egy családról számolunk be, amelyben a 6 gyermek közül két nő és egy férfi esetében jelentkezett a betegség. Míg a két nőnél szemészeti tünetek nélkül csak májérintettség volt észlelhető, addig a fiatal férfinál pszichiátriai és szemészeti eltérés volt kimutatható. Ismertetjük a klasszikus Kayser–Fleischer-gyűrű képét mutató cornealis elváltozásról modern vizsgálómódszerekkel (az elülső szegmentum optikaikoherencia-tomográfiája, corneatopográfia, endothelmikroszkópia) nyert adatokat. Felhívjuk a figyelmet a szemészeti vizsgálat fontosságára, amely segíthet a pontos diagnózis felállításában. Orv Hetil. 2019; 160(14): 555–557.

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Absztrakt:

A primer immunhiányos betegségekben az immunrendszer hibás működését genetikai eltérés okozza. A kezelőorvos a klinikai tünetek, jelek, a családi anamnézis és a kórokozó-kimutatás eredményei alapján veti fel a lehetséges diagnózisokat. A gyanú igazolásához nagy segítséget ad, ha az érintett immunológiai funkció in vitro diagnosztikai módszerekkel tesztelhető. A közlemény az antitestválasz, a T-sejtek, a phagocytafunkció, a komplementszisztéma és a veleszületett immunrendszer egyéb elemeinek vizsgálatára alkalmas szűrő, megerősítő és betegségspecifikus laboratóriumi módszereket foglalja össze, nem taglalva a végső diagnózist megadó genetikai teszteket. Orv Hetil. 2018; 159(49): 2087–2094.

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