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Phenotypic and genotypic evaluation of wheat genetic resources and development of segregating populations are pre-requisites for identifying rust resistance genes. The objectives of this study were to assess adult plant resistance (APR) of selected wheat genotypes to leaf rust and stem rust and to develop segregating populations for resistance breeding. Eight selected Kenyan cultivars with known resistance to stem rust, together with local checks were evaluated for leaf rust and stem rust resistance at seedling stage and also across several environments. Selected diagnostic markers were used to determine the presence of known genes. All eight cultivars were crossed with local checks using a bi-parental mating design. Seedling tests revealed that parents exhibited differential infection types against wheat rust races. Cultivars Paka and Popo consistently showed resistant infection types at seedling stage, while Gem, Romany, Pasa, Fahari, Kudu, Ngiri and Kariega varied for resistant and susceptible infection types depending on the pathogen race used. The control cultivars Morocco and McNair consistently showed susceptible infection types as expected. In the field, all cultivars except for Morocco showed moderate to high levels of resistance, indicating the presence of effective resistance genes. Using diagnostic markers, presence of Lr34 was confirmed in Gem, Fahari, Kudu, Ngiri and Kariega, while Sr2 was present in Gem, Romany, Paka and Kudu. Seedling resistance gene, Sr35, was only detected in cultivar Popo. Overall, the study developed 909 F6:8 recombinant inbred lines (RILs) as part of the nested mating design and are useful genetic resources for further studies and for mapping wheat rust resistance genes.
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172 25 36 Jatau ED, Olonitola OS, Olayinka AT: Prevalence of trichomonas infection among women attending antenatal clinics in Zaria, Nigeria. Annals
watermelon and the role of seed in the spread of infection. In: Sokol, P. F. (ed.), pp. 141-164. Izdat, Urozhai Kiv, Ukr. Izdat 141 164
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Several grapevine viruses were reported in Algeria and especially in grapevine germplasm collection, therefore it is a great challenge to free these varieties from virus infection before any breeding programs. Our study focused on the development of chemotherapy on autochthonous varieties collected in the grapevine germplasm collection of ITAFV. All these varieties were tested by DAS-ELISA and the presence of GLRaV-3 and GFLV was confirmed in all used samples for the sanitation. After 8 weeks of shoot tips in vitro culture in a modified M S medium containing ribavirin, DAS-ELISA test revealed that GLRaV-3 was completely eliminated and GFLV to a significant rate.
Chelladurai, V., Jayas, D. S., White, N. D. G. (2010): Thermal imaging for detecting fungal infection in stored wheat. J. Stored Prod. Res. , 46 , 174–179. White N. D. G. Thermal imaging
(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice Infect Immun 75 861 869 . 19
Campylobacter jejuni Infections: update on emerging issues and trends Clin Infect Dis 32 1201 – 1206 . 8. M.J. Blaser
