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Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.

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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Maria Gadzikowska, Anna Petruczynik, Monika Waksmundzka-Hajnos, Mirosław Hawrył, and Grzegorz Jóźwiak

M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

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M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

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. [13] S. Gurule , D. Goswami , A. Saha , Y. Modhave , A.K. Monif , Bioanalytical method development and validation using incurred samples: Quantitative estimation of

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M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997, pp. 53–71. Krull I.S. Analytical

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. [13] L.M. Gray , Analytical method development and validation of the simultaneous UV-Vis detection of fosinopril sodium, benazepril hydrochloride

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] R. Shanmugam , K. Gowthamarajan , D.L. Priyanka , K. Madhuri , V.V. Karri Narayanareddy , Bioanalytical method development and validation for herbal quercetin in nano formulation by RPU-FLC in rabbit plasm , J

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that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation has been completed. The USP (2000) defines parameters that can be used to

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into the UPLC–MS/MS system for analysis. Method Validation According to the guidelines for bioanalytical method development and validation issued by the Food and Drug Administration (FDA) [ 11 ] and European Medicines

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the analyte signal at the same retention time of an LOQ sample prepared from the same matrix. Results and discussion Method development and validation The development and validation of an analytical method for quantification of nicotine in e

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