Authors:Ekambaram Sanmuga Priya, Perumal Senthamil Selvan, and Krishnamoorthy Marudhupandian
Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.
Authors:Shraddha Jadhao, Asha Thomas, Amol Raje, Shatrughna Nagrik, Lata Kothapalli, and Aaftab Shaikh
, K. Gowthamarajan , D.L. Priyanka , K. Madhuri , V.V. Karri Narayanareddy , Bioanalytical methoddevelopmentandvalidation for herbal quercetin in nano formulation by RPU-FLC in rabbit plasm , J
Authors:Hisham Elrefay, Omnia A. Ismaiel, Wafaa S. Hassan, Abdalla Shalaby, and Ali Fouad
that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after methoddevelopmentandvalidation has been completed. The USP (2000) defines parameters that can be used to
Authors:Peiwu Geng, Xinhua Luo, Xiufa Peng, Zixia Lin, Wenhao Chen, Jin Zhang, Congcong Wen, Lufeng Hu, and Siyi Hu
into the UPLC–MS/MS system for analysis.
According to the guidelines for bioanalytical methoddevelopmentandvalidation issued by the Food and Drug Administration (FDA) [ 11 ] and European Medicines
the analyte signal at the same retention time of an LOQ sample prepared from the same matrix. Results and discussion Methoddevelopmentandvalidation The development and validation of an analytical method for quantification of nicotine in e