Authors:Ekambaram Sanmuga Priya, Perumal Senthamil Selvan, and Krishnamoorthy Marudhupandian
Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.
Authors:Shraddha Jadhao, Asha Thomas, Amol Raje, Shatrughna Nagrik, Lata Kothapalli, and Aaftab Shaikh
, K. Gowthamarajan , D.L. Priyanka , K. Madhuri , V.V. Karri Narayanareddy , Bioanalytical methoddevelopmentandvalidation for herbal quercetin in nano formulation by RPU-FLC in rabbit plasm , J
Authors:Peiwu Geng, Xinhua Luo, Xiufa Peng, Zixia Lin, Wenhao Chen, Jin Zhang, Congcong Wen, Lufeng Hu, and Siyi Hu
into the UPLC–MS/MS system for analysis.
According to the guidelines for bioanalytical methoddevelopmentandvalidation issued by the Food and Drug Administration (FDA) [ 11 ] and European Medicines
included in this article, our extensive previous experience in drug product analysis and exhaustive literature searches through SciFinder® (Chemical Abstracts), ISI Web of Science, and Google Scholar were employed to guide our methoddevelopmentand
Authors:Nitin Kumar, D. Sangeetha, L. Kalyanraman, and K. Sainath
condition on bench top for 48 h at room temperature. The freshly prepared sample and standard were injected by using the stored mobile phase at a time interval of 24 h.
Results and Discussion