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Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.

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. [13] S. Gurule , D. Goswami , A. Saha , Y. Modhave , A.K. Monif , Bioanalytical method development and validation using incurred samples: Quantitative estimation of

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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Maria Gadzikowska, Anna Petruczynik, Monika Waksmundzka-Hajnos, Mirosław Hawrył, and Grzegorz Jóźwiak

M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

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. [13] L.M. Gray , Analytical method development and validation of the simultaneous UV-Vis detection of fosinopril sodium, benazepril hydrochloride

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] R. Shanmugam , K. Gowthamarajan , D.L. Priyanka , K. Madhuri , V.V. Karri Narayanareddy , Bioanalytical method development and validation for herbal quercetin in nano formulation by RPU-FLC in rabbit plasm , J

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M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

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M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997, pp. 53–71. Krull I.S. Analytical

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into the UPLC–MS/MS system for analysis. Method Validation According to the guidelines for bioanalytical method development and validation issued by the Food and Drug Administration (FDA) [ 11 ] and European Medicines

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included in this article, our extensive previous experience in drug product analysis and exhaustive literature searches through SciFinder® (Chemical Abstracts), ISI Web of Science, and Google Scholar were employed to guide our method development and

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development and validation. The output signal (254 nm) was monitored and processed using QuickStart Empower Waters 2002 software. A Phenomenex Luna C-18 (150 × 4.6 mm, 5 μ) column was used for the HPLC studies and method development, as well as the

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