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Pressurized planar electrochromatography (PPEC) with commercially available chiral plates has been used for separation of the enantiomers of tryptophan and valine. The effects on migration distance of polarization voltage, buffer pH and concentration, and the concentration of the organic component of the mobile phase have been investigated. Solute separation is compared for PPEC and conventional planar chromatography.

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Thin-layer chromatography and electrophoresis, with their long histories of simple and effective characterization of chemical mixtures, have motivated an effort to combine these techniques. Simultaneous chromatography and electrophoresis (SCE) utilizes an electric field orthogonal to capillary action or pressure flow to achieve a single-step two-dimensional separation. In this work, plate conditioning and pressurized simultaneous chromatography and electrophoresis (pSCE) are introduced. These improvements reduce separation times and concurrently increase or maintain separation quality as described by visual comparisons, nearest neighbor distance descriptors, and relative spot capacities.

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, valine, phenylalanine) D- and L-enantiomers with high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) both with β-CD as the component of the mobile phase is reported [ 17 ]. In view of the above knowledge

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