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Orvosi Hetilap
Authors: Szimonetta Lohner, Tamás Marosvölgyi, István Burus, János Schmidt, Dénes Molnár, and Tamás Decsi

Thies, F., Miles, E. A., Nebe-von-Caron, G. és mtsai: Influence of dietary supplementation with long-chain n-3 or n-6 polyunsaturated fatty acids on blood inflammatory cell populations and functions on plasma soluble adhesion molecules in healthy

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Acta Chromatographica
Authors: Andreea Silvia Neamţu, Andrei Biţă, Ion Romulus Scorei, Gabriela Rău, Ludovic Everard Bejenaru, Cornelia Bejenaru, Otilia-Constantina Rogoveanu, Carmen Nicoleta Oancea, Antonia Radu, Cătălina Gabriela Pisoschi, Johny Neamţu, and George Dan Mogoşanu

, 10 ]. Since July 2013, NAR became accessible in dietary supplement form under the brand name NIAGEN® (Chromadex Incorporated, Irvine, California, USA). A lot of different other products that contain the compound have been commercially

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. 21 16 Maresh CM, Armstrong LE, Hoffmann JR, Hannon DR, Gabaree CLV, Bergeron MF, Whittlesey MJ, Deschenes MR: Dietary Supplementation and Improved Anaerobic

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The effect of omega–3 fatty acids in the human body is multidirectional. They are used in cancer and cardiovascular disease prevention, to stabilize blood pressure, to improve immune function, and in the treatment of concentration disorders. Omega-3 acids, especially eicosapentaenoic and docosahexaenoic acids, are needed for proper human growth. There are several groups of pharmaceutical products containing these compounds, available in pharmacies and markets. Due to a high accessibility of various consumable products such as dietary supplements or food, their quality monitoring is advisable. Therefore, analytical procedures are needed, which will allow the quality of these products to be fast and accurately defined. In our work, thin-layer chromatography (TLC) with densitometric detection was designed for the identification and quantification of the omega–3 fatty acids. The stationary phase constituted TLC silica gel plates activated by AgNO3 solution. As the mobile phase, a mixture of toluene–n-propanol–acetic acid (20:2:0.1, v/v) was used. Detection was performed at 520 nm, after staining with iodine vapor. The method was validated according to the International Conference on Harmonisation (ICH) guidelines. The correctness of the results guarantees good precision and satisfactory recovery (96.59–103.20%). The presented analytical method was used for the determination of omega-3 fatty acids in selected dietary supplements and cooking oils giving satisfactory results.

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The paper discusses a possibility to grow seeds on solutions of microelements and application of sprouts enriched in such a way as an alternative to commercial dietary supplements. It contains a short review of the approaches reported till now and a systematic experimental study, carried on the most frequently used seeds (Lens culinaris, Helianthus annuus, Vigna radiata, Glycine max, and Lepidium sativum).Seven metals (Fe, Cu, Zn, Ni, Co, Cd, and Mn) were studied. Seeds were grown on cellulose in 20°C temperature using deionized water enriched with metals in concentrations: 100, 50, 25, 12.5, 6.25, and 3.125 mg/L in a period of 4 days. The reference samples were the seeds grown on pure deionized water. Sprouts were mineralized by microwave radiation, and the metal content was quantified by ion chromatography with on-line post-column derivatization and spectrophotometric detection.The conclusions can be treated as general recommendations, which seeds should be grown and what concentrations of metals in solutions should be applied to provide good enrichment and to avoid risk of microelement overdose.

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market mainly as food or dietary supplements [ 4 ]. Consequently, neither the right choice of the products, nor their appropriate usage is controlled by healthcare professionals in all cases. Since the effect of probiotics is strain specific and greatly

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Thin-layer chromatography (TLC) is generally regarded as a common analytical technique. Everybody ‘knows’ it and it is used to some extent in almost every laboratory in the world. Unfortunately for most analysts TLC remains what it has been for many years — a rapid, simple, inexpensive, and not very precise tool. When one considers the latest technical and methodological developments, however, modern high-performance thin-layer chromatography (HPTLC), also known as planar chromatography, is a reliable and powerful analytical technique, which can be in full compliance with current good-manufacturing practice (cGMP). TLC is the method of choice when many samples must be compared, when flexibility is important, and when rapid quantitative and semi-quantitative data are needed at low cost per sample.

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References [1] US Food and Drug Administration , 21 CFR Part 111, Current good manufacturing practice in manufacturing, packaging, labeling, or holding operations for dietary supplements: final

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pharmaceuticals and dietary supplements have risen. In this context, up to 17-fold and 13-fold differences in hypericin and pseudohypericin amounts, respectively, are reported in several Hypericum -derived commercial products ( Murthy et al., 2014 ). The first

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, (trade name Levitra), and tadalafil (trade name Cialis) [ 7 , 8 ]. Another class of substances frequently subjected to counterfeiting is herbal dietary supplements for weight loss. The use of herbal products for these two types of adjustments has

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