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Introduction Enterococci are Gram-positive facultative anaerobic bacteria and they are commensals of the intestinal tract of healthy humans and animals including invertebrates ( Fisher and Phillips, 2009; Bender et al., 2018 ). Due to their
A comparison of the results obtained using the classical methods with those of the API 20 Strep system was carried out in identifying 24 enterococci strains isolated from San Simón cow's milk cheese, a traditional Spanish variety. The results of both identification systems coincided exactly in 9 strains (37.5% of the strains studied). In one strain the results obtained using the classical methods did not coincide with those using the API 20 Strep method. 3 strains (12.5%) could not be identified using the API 20 Strep system. However, 11 strains (45%), that remained doubtful between both species E. faecalis and E. faecium on the basis of the classical methods, were identified using the API 20 Strep system. The API 20 Strep system does not include some biochemical tests of importance in identifying of foodborne enterococci and could not identify the atypical strains of Enterococcus. Moreover, this system is adapted to the identification of enterococci of clinical origin and their database does not include some species common in foods. However, it could have an application in combination with the classical methods in order to carry out a reasonably rapid and reliable identification of enterococci related to cheese.
] 9 Al-Nassir, W. N., Sethi, A. K., Li, Y., et al.: Both oral metronidazole and oral vancomycin promote persistent overgrowth of vancomycin-resistant enterococci during treatment of
, Germany) were used (different types for staphylococci, enterococci and for P. aeruginosa ). Samples were inoculated on the three different media within 1–2 h after collection. From each Petri dish, one colony was used for further studies except for dishes
, Germany) were used (different types for staphylococci, enterococci and for P. aeruginosa ). Samples were inoculated on the three different media within 1–2 h after collection. From each Petri dish, one colony was used for further studies except for dishes
and water habitats [ 1 ]. Some Enterococcus species are used as probiotics to treat diarrhea and improve host immunity [ 2 ]. Enterococci are opportunistic pathogens for humans and animals. Enterococcus faecalis and Enterococcus faecium
