Search Results

You are looking at 11 - 20 of 88 items for :

  • "microsatellite markers" x
  • Biology and Life Sciences x
  • All content x
Clear All

As locus-specific co-dominant PCR-based markers that allow semi-automated, high-throughput investigation technologies, microsatellites are ideal tools for genotype identification. Eleven of a set of 114 microsatellite markers available at the Agricultural Biotechnology Center proved to be suitable to distinguish between the parents of at least one of nine sweet pepper hybrid combinations. Markers with the highest information capacity were found to be capable of distinguishing between the parents of four different hybrid combinations and exhibited up to four different alleles in 18 haplotypes.

Restricted access
Cereal Research Communications
Authors: O. Tereshchenko, E. Gordeeva, V. Arbuzova, A. Börner, and E. Khlestkina

1 174 181 Plaschke, J., Ganal, M.W., Röder, M.S. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers

Restricted access

38 1397 1403 Leonova, I., Börner, A., Budashkina, E., Kalinina, N., Unger, O., Röder, M., Salina, E. 2004. Identification of microsatellite markers

Restricted access

By employing in silico tools, we devised new chloroplast microsatellite primers for inferring phylogenetic relationships within Brassicaceae. Microsatellite repeats were scanned in 12 chloroplast genomes of Brassicaceae, regions flanking these repeats were aligned and 19 universal primers were designed. Fifteen of these primer pairs are predicted to yield polymorphic amplicons, that are more or less evenly distributed throughout the chloroplast genomes. Finally, using PCR, we have validated three primer pairs on a limited ‘test set’ of plants, different from those used in computational analysis.

Restricted access

–135. Plaschke, J., Ganal, M.W., Röder, M.S. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 191 :1001–1007. Röder M

Restricted access
Cereal Research Communications
Authors: L.R. Vemireddy, N. Ranjithkumar, A. Vipparla, M. Surapaneni, G. Choudhary, K.V. Sudhakarrao, and E.A. Siddiq

India bred high yielding rice varieties have enriched to a great extent the global rice germplasm since the mid-sixties. Systematic research efforts for development of cultivar-specific DNA fingerprints of major Indian rice cultivars, however, have not received due attention. The present investigation was aimed at development of DNA fingerprints for 90 high yielding rice varieties using hypervariable microsatellite (hvRM) markers. A panel of eight markers, viz. RM11313, RM13584, RM15004, RM5844, RM22250, RM22565, RM24260 and RM8207 was chosen from 52 polymorphic markers based on their highly polymorphic nature, SSR repeat type and number and ability to distinguish genotypes, in order to develop DNA fingerprints of 90 varieties. The remaining high polymorphic hvRM markers could be of immense value in future to distinguish new cultivars, in case they could not be distinguished by the 8 marker panel. Four of the 8 markers, viz. RM22250, RM13584, RM24260 and RM5844 were located in expressed genes and could be of value in DUS (Distinctness, Uniformity and Stability) testing. Thus we suggested, that this set of 8 loci could be used as standard for DNA fingerprinting of Indian rice cultivars.

Restricted access
Cereal Research Communications
Authors: Katalin Puskás, Gyula Vida, Judit Komáromi, Hermann Bürstmayr, Marc Lemmens, Zoltán Bedo, and Ottó Veisz
Restricted access

Huang, X. Q., Börner, A., Röder, M. S., Ganal, M. W. 2002. Assessing genetic diversity of wheat ( Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105 :699–707. Ganal M

Restricted access

Khlestkina, E.K., Röder, M.S., Efremova, T.T., Börner, A., Shumny, V.K. 2004b. The genetic diversity of old and modern Siberian varieties of common spring wheat determined by microsatellite markers. Plant Breed. 123 :122

Restricted access