Authors:R. Aggarwal, S. Purwar, L. Kharbikar, and Sangeeta Gupta
Aggarwal, R., Das, Soma, Jahani, Mehdi and Singh, D. V. (2008): Histopathology of spotblotch [ Bipolaris sorokiniana (teleomorph: Cochliobolus sativus )] infection in wheat. Acta Phytopathologica et Entomologica Hungarica 43, 23
Authors:L. Tyryshkin, M. Gashimov, M. Kolesova, and N. Anphilova
Tyryshkin L.G. and N.N. Solovieva, 2001. Resistance of barley
L. to leaf rust, dark-brown leaf spotblotch and common root rot: methods of evaluation and characteristics of collection samples. Tr. po prikl. bot., gen. i sel. 159: 93
Comparison of two storage techniques (Castellani’s method and freezing) was carried out for 16 isolates of four Fusarium head blight species and 54 isolates of Cochliobolus sativus causing spot blotch and common root rot. The viability, purity and morphological stability of cultures were verified for a one year period. Duplicate isolates were stored as a fungal suspension in sterile distilled water at 4 °C, and fungal cultures by freezing at –16 °C. The viability of 70 isolates was 100% for the two storage methods. Morphological alternations and pathogenic contaminations were not detected. All isolates were able to retain viability, purity and morphological stability by the two tested storage methods for over 12 months. Castellani’s method and freezing were found to be safe, easy, inexpensive and reliable.
Aggarwal, R., Tewari, A. K., Srivastava, K. D. and Singh, D. V. (2004): Role of antibiosis in the biological control of spotblotch ( Cochliobolus sativus ) of wheat by Chaetomium globosum. Mycopathologia 157
Aggarwal, R. (2000): Annual Report, Cess Fund Project on "Biological Control of Spotblotch ( Drechslera sorokiniana ) of wheat using Chaetomium globosum . Division of Plant Pathology, IARI, New Delhi, 13 pp