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, N. 2005. SSR marker of wheat stripe rust resistance gene Yr2 . Journal of Triticeae Crops (in Chinese) 25 :17–19. Li N. SSR marker of wheat stripe rust resistance gene Yr

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Aegilops sharonensis (Sharon goatgrass) is a valuable source of novel high molecular weight glutenin subunits, resistance to wheat rust, powdery mildew, and insect pests. In this study, we successfully hybridized Ae. sharonensis as the pollen parent to common wheat and obtained backcross derivatives. F1 intergeneric hybrids were verified using morphological observation and cytological and molecular analyses. The phenotypes of the hybrid plants were intermediate between Ae. sharonensis and common wheat. Observations of mitosis in root tip cells and meiosis in pollen mother cells revealed that the F1 hybrids possessed 28 chromosomes. Chromosome pairing at metaphase I of the pollen mother cells in the F1 hybrid plants was low, and the meiotic configuration was 25.94 I + 1.03 II (rod). Two pairs of primers were screened out from 150 simple sequence repeat markers, and primer WMC634 was used to identified the presence of the genome of Ae. sharonensis. Sequencing results showed that the F1 hybrids contained the Ssh genome of Ae. sharonensis. The sodium dodecyl sulfate polyacrylamide gel electrophoresis profile showed that the alien high molecular weight glutenin subunits of Ae. sharonensis were transferred into the F1 and backcross derivatives. The new wheat-Ae. sharonensis derivatives that we have produced will be valuable for increasing resistance to various diseases of wheat and for improving the quality of bread wheat.

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mapping of 2240 new SSR markers for rice ( Oryza sativa L.). DNA Res. 9 :199–207. Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L

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., Xing, Y., Zhang, Q., Kono, I., Yano, M., Fjellstrom, R., DeClerck, G., Schneider, D., Cartinhour, S., Ware, D., Stein, L. 2002. Development and mapping of 2240 new SSR markers for rice ( Oryza sativa L.). DNA Res. 9 :199

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Chen, H.M., Li, L.Z., Wei, X.Y., Li, S.S., Lei, T.D., Hu, H.Z., Wang, H.G., Zhang, X.S. 2005. Development, chromosome location and genetic mapping of EST-SSR markers in wheat. Chinese Sci. Bull. 50 :2328

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515 527 Song, Q.J., Shi, J.R., Singh, S., Fickus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.B. 2005. Development and mapping of microsatellite (SSR) markers

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, P.G. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110 :550–560. Cregan P.G. Development and mapping of microsatellite (SSR

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Baiftlik Beyazi potato landraces using SSR markers . Turk. J. Field Crops 15 ( 1 ): 84 – 88 . Kara , K. , Mezghani , N. , Saddoud Debbabi , O. , Madini , M. , Rached-Kanouni , M

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SSR markers. Genet. Resour. Crop Evol. 56 :499–506. Hassani M.E. Genetic diversity of the D-genome in T. aestivum and Aegilops species using SSR markers

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, H.M. , Li , L.Z. , Wei , X.Y. , Li , S.S. , Lei , T.D. , Hu , H.Z. , Wang , H.G. , Zhang , X.S. 2005 . Development, chromosome location and genetic mapping of EST-SSR markers in wheat . Chinese Sci. Bull. 50 : 2328 – 2336

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