Authors:Mieczysław Sajewicz, Robert Piętka, Gabriela Drabik, Ewa Namysło, and Teresa Kowalska
We have previously discussed in detail thin-layer chromatographic studies of the retention of the 2-arylpropionic acids (2-APA)
-(±)-2-phenylpropionic acid. Chiral TLC was performed on commercial silica gel layers impregnated with l
-arginine in the cationic form as chiral ion-pairing reagent. Retention and separation of the species was checked by densitometry.In this paper we report the striking skewed migration of these chiral APA in these TLC systems. Scanning of the chromatographic plates at 1-mm intervals revealed that the tracks of the
-(+) and the
-(−) APA can deviate markedly from the vertical in mutually opposite directions. This striking effect in the two-dimensional separation of the enantiomers is most probably caused by stereospecific intermolecular interactions which occur during ion-pair formation between l
-arginine in the cationic form, deposited on the silica gel layer as chiral selector, and each individual 2-APA in the anionic form.
The chromatographic behavior of the phenolic drugs niclosamide, hexachlorophene, ibuprofen, pentazocine, ethamivan, bithionol, salicylanilide, caffeic acid,
-coumaric acid, 4-aminosalicylic acid, ferulic acid, and methyldopa has been investigated on RP8F
TLC plates with methanol-water mixtures in different volume proportions as mobile phases. Linear relationships were obtained between the
values of the drugs and the volume fraction of methanol in the mobile phase. Retention values,
, were extrapolated to zero methanol content and the lipophilicity values
obtained were compared both with measured partition coefficients (log
) and with partition coefficients (AlogPs, IAlogP, AB/logP, COSMOFFrag, miLogP, KOWWIN, and xlogP) calculated using seven different software products. Comparison of the calculated partition coefficients revealed IAlogP, KOWWIN, miLogP, and
(where is the average of all the theoretical partition coefficients) usually correlate best with chromatographic lipophilicity
. The results indicate that chromatographic lipophilicity
can be used as a measure of the lipophilicity of the phenolic drugs investigated.
Authors:Mieczysław Sajewicz, Monika Gontarska, Dorota Kronenbach, and Teresa Kowalska
Our earlier thin-layer chromatographic and polarimetric investigations enabled discovery of the spontaneous in-vitro oscillatory chiral inversion of the profen drugs
-(−)-flurbiprofen, etc., and then of the α-amino acids l
-alanine, and l
-tyrosine. In those investigations, thin-layer chromatography convincingly demonstrated its potential as a flexible and handy tool in the service of physical organic chemistry in general and investigation of organic reaction mechanisms in particular. Later — largely on the basis of thin-layer chromatographic evidence — we proposed a reaction-diffusion model that may provide the core of a mechanistic understanding of the spontaneous oscillatory in-vitro chiral inversion of profens and α-amino acids. In this study, we present thin-layer chromatographic and polarimetric evidence of the analogous process of the oscillatory chiral in-vitro inversion of
-(+)-ketoprofen, which is meant to expand an already existing database, mostly originating from our laboratory and documenting the universal nature of this process with α-substituted chiral propionic acid derivatives (in the first instance, profen drugs and α-amino acids).
The chromatographic separation of fenbufen, ibuprofen, ketoprofen, diclofenac sodium, mefenamic acid, and tiaprofenic acid has been investigated. Normal-phase chromatography on silica gel by the ascending and horizontal techniques, and reversed-phase chromatography on octadecyl-bonded silica gel (RP-18) in horizontal chambers, were performed with suitable mobile phases. The substances were identified by UV illumination at
= 254 nm and by use of dyeing reagents. Reversed phase chromatography with phosphate buffer, pH 5.73–10% CTMA-Br in methanol, 3.5 + 6.5 (
), as mobile phase enabled better separation of the six drugs than normal-phase mode. A simple videodensitometric TLC method on silica gel RP-18 was developed and validated for quantitative determination of fenbufen in tablets. The limits of detection and quantification were determined by videodensitometry at
= 254 nm. A calibration plot was constructed in the range 2.0–12.0 μg/5-μL spot and was linear with a good correlation coefficient (0.9926).
for quantitation of fenbufen were from 2.44 to 3.10%. The method was applied satisfactorily to pharmaceutical preparations.
Transfer of four rapid thin-layer chromatography (TLC) screening methods used to detect substandard and counterfeit pharmaceutical products to quantitative high-performance TLC (HPTLC)-densitometry methods is demonstrated. These methods for acetaminophen, acetylsalicylic acid, ibuprofen, and chlorpheniramine maleate are contained in a Compendium of methods developed by Kenyon and Layloff for use in countries with limited resources. The new quantitative methods use Merck HPTLC silica gel 60 F254 glass plates, automated standard and sample application, and automated densitometry for detection, identification, and quantification. Standard and sample solution preparation and application procedures for obtaining calibration curves and bracketed samples are described. The HPTLC plates give better efficiency, selectivity, and resolution than TLC, and the new methods overcome the deficiencies in technology related to manual application and visual zone comparison that do not allow the Compendium TLC procedures to support regulatory compliance actions. These transferred methods can be fully validated according to International Conference on Harmonization (ICH) guidelines or by interlaboratory studies if their applications require. The approach described can be used to transfer the remaining Compendium methods as well as the GPHF [Global (formerly German) Pharma Health Fund E.V.] Minilab kit TLC screening methods.
Authors:C. Negro, L. Longo, G. Vasapollo, L. Bellis, and A. Miceli
Four ecotypes of pomegranate fruits growing in the Salento area were characterized for their content in terms of total phenols, sugars, organic acids, anthocyanins, antioxidant and anti-inflammatory activity. The results showed that in the fruit juice the amount of phenols ranged between 0.8–1.7 g l−1; the content in terms of simple sugars was about 140 g l−1, while the amount of citric and malic acids ranged between 1.4–13.3 and 0.8–7.7 g l−1, respectively. Seven anthocyanin pigments were found; these were analysed quantitatively and qualitatively by HPLC-DAD-MS and identified as delphinidin 3,5-diglucoside, cyanidin 3,5-diglucoside, pelargonidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, pelargonidin 3-glucoside and cyanidin 3-arabinoside. The antioxidant activity, determined as DPPH scavenger and TEAC, ranged between 17.2 and 39.1% and between 9 and 19 μmol Trolox per ml of juice, respectively. All the pomegranate juices showed an anti-inflammatory activity (measured by COX inhibitory assay) which was compared with two commercial anti-inflammatory drugs (Ibuprofen and Nimesulide).
Authors:Alan Ramić, Marica Medić-Šarić, Srećko Turina, and Ivona Jasprica
Thin-layer chromatography has been used to investigate possible chemical interactions of vitamins A and D with frequently used therapeutics (estrogens and progestins, corticosteroids, HMG CoA reductase inhibitors, vitamins, and nonsteroidal anti-inflammatory drugs). We also compared our results with biochemical interactions found in the literature. Concentrations of vitamins and drugs applied to the plate were adjusted to mimic the doses usually prescribed in therapy. TLC was performed on 10 cm × 20 cm TLC plates precoated with silica gel 60 F
. The first step was to determine
values for each vitamin and drug using three different mobile phases — cyclohexane-ether, 50 + 50 (
), cyclohexane—ether, 85 + 15 (
), and ethyl acetate. Solutions of vitamins A and D were applied to the plates as 8 mm bands and the investigated drugs were applied as spots. Chromatography was performed in ascending mode. After calculation of
values we used a combination of stationary and mobile phase for which overlapping of the vitamin band and drug spot occurred during chromatogram development. The strength of interaction was measured as a surface below or above the distorted part of the sample band, visible under short wavelength UV light (
= 254 nm) or after exposing the plates to iodine vapor. The chromatograms were documented by use of a Camag Reprostar 3 System.We established the occurrence of chemical interactions between vitamin A and estradiol propionate, tocopherol acetate, ibuprofen, and ketoprofen, and between vitamin D and hydrocortisone, medrox-yprogesterone acetate, norethisterone acetate, aspirin, ketoprofen, and acetaminophen. A clear relationship between chemical and biochemical interactions could not be established.
Authors:Viktor József Horváth, Gy. Ádám Tabák, Gergely Szabó, Zsuzsanna Putz, Csaba Géza Koós, and Péter Lakatos
placebo-controlled, ex vivo, serial placebo-controlled serial crossover study. Eur. J. Clin. Pharmacol., 2013, 69 (3), 365–371.
Curtis, J. P., Wang, Y., Portnay, E. L., et al.: Aspirin, ibuprofen, and mortality after
studies. PLoS Med. 2011; 8: e1001098.
European Medicines Agency. Updated advice on use of high-dose
ibuprofen. EMA/325007/2015. Available from: