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Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.

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Abstract  

The reaction14N(p, n)14O(71 s) is used, with a proton beam of 14 MeV energy and 100 nA intensity. The14O activity is detected by means of its characteristic 2.312 MeV gamma-ray (9.4%), with a NaI(T1) detector. The intensity ratio of gamma-rays to that of the incident particles for the sample, related to a similar ratio in adequate standards determines the nitrogen content in the sample. The measurement is completely automated. If we protect the embryo region during irradiation, the future germination of the analyzed seeds will be normal. Good correlation has been obtained between the results of the present method and those of the chemical Kjeldahl method.

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The effects of 100, 250, and 500 ppm acetylsalicylic acid solutions treatments on weight alteration, pigment and protein amounts in discs from the primary leaves of one month old bean (Phaseolus vulgaris L.) seedlings produced under greenhouse conditions are presented. The experiments show that: 100 ppm ASA had no significant influence (P?0.05) but 250 and 500 ppm ASA caused an increase on weight loss (P<0.01); ASA at higher concentrations (250 and 500 ppm), generally, caused a decrease on pigment amounts (P<0.05-P<0.01) but 100 ppm ASA had no considerably significant influence on them (P?0.05), none of the ASA treatments caused a statistically significant influence on carotenoid amount (P?0.05); 100 and 250 ppm ASA treatments did not cause a significant influence on protein amount (P?0.05), however 500 ppm ASA treatment caused an increase on protein injury (P<0.05). Consequently, it is supposed that wet weight loss, pigment and protein injury have somewhat increased on leaf discs, depending on the toxic effect of high acetylsalicylic acid concentrations.

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Wang, L., Licheng, H. (1995): Breeding for new spring soybean cultivar Heinong 35 with high protein content and high yield and problems on soybean breeding for dwarf and other mutants. Sci. Agr. Sinica , 28 , 38

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84 201 218 Ceapoiu, N., Ittu, G., Oproiu, E. 1974. Ereditatea conţinutului şi calitaţii proteinelor la grâu [Heredity of protein content and quality

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Wang, J-M., Zhang, G-P. and Chen, J.X. (2001). Cultivar and environmental effects on protein content and grain weight of malting barley. Journal of the Zhejiang University (Agriculture and Life Sciences) 27: 503

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The unfavourable effects of climate change were studied in terms of changes in the stress tolerance of cereals. The yield and physiological parameters of two winter wheat genotypes (Mv Mambó, Mv Regiment) were analysed in the phytotron after water was completely withheld for 7 or 14 days in three phenophases. The plants were raised in climate chambers, one adjusted to ambient CO2 concentration and the other to a higher level (750 μmol mol−1). The aim of the present work was to determine the correlations between the duration of water withholding and the phenological, physiological and yield parameters of winter wheat. It was hoped to identify how elevated CO2 levels affected the stress sensitivity of plants and whether they contributed to counteracting the damaging effects of drought. In both varieties, the grain mass decreased to the greatest extent when water was withheld at first node appearance (5.9–71.3%). A longer period of drought at first node appearance and grain filling only reduced the grain number and mass in the case of enhanced CO2. The yield and physiological parameters of Mv Regiment, however, deteriorated substantially as a result of water deficiency, though this variety was better able to utilise surplus CO2, giving outstanding results at elevated CO2 level.

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Izsáki, Z. (1999): A nitrogén és foszfor ellátottság hatása néhány szántóföldi kultúra fehérjetartalmára és aminosav összetételére. (Effect of N and P supplies on protein content and amino acid composition of some field crops.) In: Ruzsányi, L., Pepó, P

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–124. Cyran, M., Bona, L., Boros, D., Hajós, G. 2002. Dietary fiber composition, viscosity and protein content of winter and spring cereals. In: Arseniuk, E. (ed.), Proc. 5th Int. Triticale Symp., International Triticale Association, Radzikow, Poland, pp. 438

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