, D, and E viruses, transfusion transmitted virus, herpes simplex virus, cytomegalo virus, and Epstein–Barr virus, can lead to FH, liver dysfunction, and jaundice in pregnancy universal, as well as the clinical features, have implicated a variant of
's instructions (Qiagen, USA). OXA-48, KPC, NDM-1, IMP, and VIM-1 carbapenemases were identified by PCR amplification and sequencing as described previously [ 14 ]. The colistin resistant isolates were screened by simplex PCRs for the presence of mcr-1, mcr-2
Becker, Y., Hadar, J., Tabor, E., Ben-Hur, T., Raibstein, I., Rosen, A., et al.: A sequence in HpaI-P fragment of herpes simplex virus-1 DNA determines intraperitoneal virulence in mice. Virology 149 , 255
Authors:Yasemin Baskin, Z. Yazici, H. Baskin, A. Ozkul, Nur Olgun, and I. H. Bahar
. (1999): Induction and prevention of apoptosis in human HEp-2 cells by herpes simplex virus type 1. J. Virol. 73, 10359-10370.
Induction and prevention of apoptosis in human HEp-2 cells by herpes simplex virus type 1
Authors:M. Yazdanpanah, Mojgan Mokhtari, K. Mostofi, M. Soleimani, M. Ebrahimirad, H. Esmaili, and Sara Ahmadi
Oral poliovirus vaccine (OPV) is reported to be effective in treatment of recurrent herpes simplex (RHS). According to our observation during recent years, OPV was not only effective in management of RHS but also in some patients with concomitant recurrent aphthous stomatitis (RAS) reducing its severity and frequency. The purpose of this study was to evaluate the efficacy of OPV in the management of RAS.In a longitudinal, case — control study 48 patients with RAS were recruited. Twenty patients received OPV and 28 patients received placebo. OPV was administered in a dose of 4 drops at monthly intervals for 3 months to the study group while the control group received placebo. The results were registered in 3 months after the last dose.Eight cases (40%) in the OPV group showed significant reduction in the duration of the ulcers, while no change was seen in the control group (P = 0.048). The frequency of recurrence of RAS was reduced in 13 cases (65%) in the OPV group, and in 6 cases (21.4%) of the placebo group (P = 0.006). The severity of attacks was reduced in 12 cases (60%) in the OPV group and in 4 cases (14.3%) in the placebo group (P = 0.008).In conclusion OPV appeared to be effective in the management of RAS.
Authors:Adauto Chiamenti, Cristiano Filho, Marcelo Moura, Fabíola Paula-Lopes, Jairo Neves, Cícero Neto, Paulo Gonçalves, Paulo Lima, and Marcos Oliveira
Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and retinoic acid (RA) on goat oocyte maturation as well as the effects of insulin-like growth factor-I (IGF-I), RAc and RA during embryo culture under chemically defined conditions. In Experiment 1, in vitro maturation (IVM) was performed in a chemically defined basic maturation medium (bMM) supplemented with 0.3 μM RAc or 0.5 μM RA. Presumptive zygotes and embryos (2–4 cells) were cultured in droplets of potassium simplex optimised medium (KSOM); however, none of the embryos reached the blastocyst stage. In Experiment 2, oocytes were matured in bMM + RAc or bMM + RA. Presumptive zygotes and 2- to 4-cell embryos were placed in fresh KSOM droplets supplemented with RAc, RA, IGF-I, RAc+IGF-I or RA+IGF-I. In Experiment 1, addition of RAc and RA to bMM increased (P < 0.05) the proportion of 2- to 4-cell embryos reaching the morula stage as compared to the control. In Experiment 2, supplementation of embryo culture media with retinoids and IGF-I increased (P < 0.05) the proportion of 2- to 4-cell stage embryos developing to the morula and blastocyst stage. Our data demonstrate that goat embryo production in chemically defined media could be improved by exogenous RAc or RA and by the interaction between retinoids and IGF-I, and that goat embryos can be produced in vitro from oocytes following protocols similar to those currently used for cattle.
Fawl, R. L., Gesser, R. M., Valyi-Nagi, T. and Fraser, N. W. (1996): Reactivation of herpes simplex virus from latently infected mice after administration of cadmium is mouse-strain-dependent. J. Gen. Virol. 77, 2781