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Cereal Research Communications
Authors: W.F. Song, Z.Y. Ren, Y.B. Zhang, H.B. Zhao, X.B. Lv, J.L. Li, C.H. Guo, Q.J. Song, C.L. Zhang, W.L. Xin, and Z.M. Xiao

Two lines, L-19-613 and L-19-626, were produced from the common wheat cultivar Longmai 19 (L-19) by six consecutive backcrosses using biochemical marker-assisted selection. L-19 (Glu-D1a, Glu-A3c/Gli-A1?; Gli-A1? is a gene coding for unnamed gliadin) and L-19-613 (Glu-D1d, Glu-A3c/Gli-A1?) formed a set of near-isogenic lines (NILs) for HMW-GS, while L-19-613 and L-19-626 (Glu-D1d, Glu-A3e/Gli-A1m) constituted another set of NILs for the LMW-GS/gliadins. The three L-19 NILs were grown in the wheat breeding nursery in 2007 and 2008. The field experiments were designed using the three-column contrast arrangement method with four replicates. The three lines were ranked as follows for measurements of gluten strength, which was determined by the gluten index, Zeleny sedimentation, the stability and breakdown time of the farinogram, the maximum resistance and area of the extensogram, and the P andWvalues of the alveogram: L-19-613 > L-19-626 > L-19. The parameters listed above were significantly different between lines at the 0.05 or 0.01 level. The Glu-D1 and Glu-A3/Gli-A1 loci had additive effects on the gluten index, Zeleny sedimentation, stability, breakdown time, maximum resistance, area, P and W values. Although genetic variation at the Glu-A3/Gli-A1 locus had a great influence on wheat quality, the genetic difference between Glu-D1d and Glu-D1a at the Glu-D1 locus was much larger than that of Glu-A3c/Gli-A1? and Glu-A3e/Gli-A1m at the Glu-A3/Gli-A1 locus. Glu-D1d had negative effects on the extensibility and the L value compared with Glu-D1a. In contrast, Glu-A3c/Gli-A1? had a positive effect on these traits compared with Glu-A3e/Gli-A1m.

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., Békés, F. 2011. A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary. Cereal Res. Commun. 39 :226–237. Békés F. A retrospective analysis

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Li, X.H., Ma, W., Gao, L.Y., Zhang, Y.Z., Wang, A.L., Ji, K.M., Wang, K., Appels, R., Yan, Y. 2008. A novel chimeric LMW-GS gene from the wild relatives of wheat Ae. kotschyi and Ae. juvenalis : evolution at the Glu-3 loci. Genetics

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1683 Gupta, R.B., Shepherd, K.W. 1990. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor. Appl

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Hareland, G. (2004): Durum wheat products. In: Proc. 3 rd Food Sci. Int. Symp, pp. 26. He, Z.H., Liu, L., Xia, X.C., Liu, J.J., Pena, R.J. (2005): Composition of HMW and LMW Glutenin subunits and their effects on

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weight (HMW) and low molecular weight (LMW) glutein subunits controlled by Glu-1 and Glu-3 loci on durum wheat quality. Cereal Chem. 78 :59–63. Carrillo J.M. Influence of high

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., Ateş Sönmezoğlu, O., Sayaslan, A., Koyuncu, M., Güleç, T., Kandemir, N. 2013. Marker-assisted breeding of a durum wheat cultivar for γ -gliadin and LMW-glutenin proteins affecting pasta quality. Turkish J. of Agriculture and Forestry 37 :1

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29 Singh, N.K., Shepherd, K.W., Cornish, G.B. 1991. A simplified SDS-PAGE procedure for separating LMW-GS. J. Cereal Sci. 14 :203–208. Cornish

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Acta Alimentaria
Authors: M. Oszvald, S. Tömösközi, L. Tamás, and F. Békés

81 Gupta, R.B. & Shepherd, K.W. (1993): Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin. 1. Variation and genetic control of the subunits in

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Clarke B.C., Phongkham T., Gianibelli M.C., Beasley H., Bekes F. (2003) The characterisation and mapping of a family of LMW-gliadin genes: effect on dough properties and bread volume. Theor. Appl. Genet. 106: 629

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