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Oilseeds, March 08–10, Toledo, Spain. EAAP European Association for Animal Production Publication, Wageningen Academic Publ., Wageningen, pp. 143–147. Kelly D

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, the emulsified meat products always have high fat content, typically 20–30% of animal fats with abundant cholesterol and saturated fatty acid (SFA) ( Herrero et al., 2014 ). Awareness on consumption of high fat food products, especially with high

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, J.M. , Leeas , M. & Sloance-Stanley , G.H. ( 1957 ): A simple method for the isolation and purification of total lipids from animal tissues . J. Biol. Chem. , 226 , 495 – 509 . IOM ( 2005 ): Dietary

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Folch, J. , Lees, V. & Sloane-Stanley, G.H. (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. , 226 , 497–506. Sloane-Stanley G

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, National Health Surveillance Agency. B RAZIL ( 2003 ): Métodos analíticos oficiais para análises microbiológicas para controle de produtos de origem animal e água (Official analytical methods for microbiological

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): Task Force Report no. 139 Mycotoxins: Risks in Plant, Animal and Human Systems. Council for Agricultural Science and Technology, Ames, Iowa, USA. Task Force Report no. 139 Mycotoxins: Risks in

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, M., Paredes, S., Pavez, H., Rodríguez, A.B. & Barriga, C. (2006b): The oral administration of tryptophan improves nocturnal rest in young animals: Correlation with plasma melatonin. Biogen. Amines. , 20 (1–2), 53

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. & Crackel, R. L. (1991): Lipid oxidation in meat products, -in: Coelho, M. B. (Ed.) Vitamin E in animal nutrition and management. BASF, New Jersey, USA, pp. 147-153. Vitamin E in animal nutrition and management

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Acta Alimentaria
Authors: S. Dubná, V. Rada, E. Vlková, V. Hořejšová, and J. Havlík

An animal protein-free culture medium was developed for the cultivation of bifidobacteria. The medium is based on fermented wheat germ (wheat germ medium; WGM), which serves as a good source of amino acids for bacteria. The number of colonies, changes in pH and optical density were compared with commercial Wilkins-Chalgren (WCB) broth and a nonmilk-based general edible medium (GEM). All bifidobacterial strains (n=9) displayed good growth on WGM. Strains of Bifidobacterium animalis, B. longum and B. breve grew on all three media tested. On the contrary, B. bifidum did not grow on GEM. WGM seems to be suitable medium for the propagation of bifidobacteria and for the production of animal protein-free probiotic food.

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The aim of this work was to identify new issues of the information demanded by beef consumers toward voluntary labelling. For this purpose, a total of 353 surveys were carried out to beef buyers who reside in three Spanish cities. To analyse the consumers’ demand information we used Chi-square tests. The results showed that information about sanitary and waste analysis and animal-rearing methods were the most demanded by consumers. Also, we found that there were clear links between the demand for information on the voluntary labelling of fresh beef and sociodemographic variables, such as gender, age, level of education, and the family income of the respondents.

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