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.A.P. , Selamat, A. 2012 . Molecular characterization of tropical sweet corn inbred lines using microsatellite markers . Maydica 57 : 154 – 63 . Menkir , A. , Badu-Apraku , B

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. Appl. Genet. 112 : 1239 – 1247 . Gorji , A.H. , Zolnoori , M. 2011 . Genetic diversity in hexaploid wheat genotypes using microsatellite markers . Asian J. of

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microsatellite markers. Acta Horticulturae 603 , 59–66. Dettweiler E. EU-Project Genres CT96 No81: European Vitis database and results regarding the use of a common set of microsatellite

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. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat . Genome 43 : 689 – 697 . Pretorius , Z.A. , Singh , R.P. , Wagoire , W.W. , Payne , T

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. , Ben Naceur , M. 2016 . Assessment of genetic diversity of wheat ( Triticuma estivum L.) using agro-morphological characters and microsatellite markers . International Journal of Biosciences 9 ( 4 ): 92 – 101

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. Choudhary , M. , Hoßsain , F. , Muthusamy , V. , Thirunavukkarasu , N. , Saha , S. , Pandey , N. , Jha , S.K. , Gupta , H.S. 2015 . Microsatellite marker-based genetic diversity analyses of novel maize inbreds poßsessing rare

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Sphagnum based on microsatellite markers are reported as of 0.594 for S. cossonii , 0.457 for S. revolvens ( Kophimai et al . 2014 ), 0.174 for S. junghuhnianum , 0.367 for S. palustre and 0.298 for S. imbricatum . Korpelainen et al . (2008

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microsatellite markers for the European corn borer, Ostrinia nubilalis Hübner (Lepidop-tera: Crambidae). Molecular Ecology Notes 6, 945–947. 7 Devereux, J., Haeberli, P. and Smithis O

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Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1F1 and BC2F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.

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The objectives of this research were to examine the inheritance of leaf rust resistance genes in the Iranian wheat cultivar ‘Marvdasht’, which is highly resistant to leaf and stripe rusts, and to identify Lr genes present in this cultivar using molecular markers. The genetic basis of resistance to the leaf rust pathogen (Puccinia triticina) in ‘Marvdasht’ was studied in F2:3 populations derived from crosses of Bolani (susceptible cultivar) × Marvdasht. Isolates 84-1 and 85-28 of P. triticina, which are the predominant isolates in Iran, were used to examine the segregation of resistance originating from ‘Marvdasht’. The results indicated that resistance in ‘Marvdasht’ to Puccinia triticina isolate 84-1 was governed by two dominant seedling resistance genes Lr1 and Lr17a. Allelism studies using an F2 population derived from a cross between ‘Falat’ (Seri 82) and Marvdasht indicated that resistance in Marvdasht was not due to the resistance gene Lr26 present in ‘Falat’. With the application of a previously developed molecular marker for Lr1, the STS marker RGA-567-5, the presence of Lr1 was verified in Marvdasht. Based on bulk segregant analysis, Lr17a was mapped to the distal end of chromosome 2AS and was closely linked to microsatellite marker Xbarc212 at a distance of 3.7 cM. In conclusion, the presence of Lr1 and Lr17a was confirmed in the cultivar Marvdasht.

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