from Sigma-Aldrich, St. Louis (Missouri, USA). Precoated TLC (silica gel 60 F254) and quercetin were purchased from Merck (Darmstadt, Germany). Rutin and β-sitosterol were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). The solvents used for
Authors:Yuping Shen, Minhui Xu, Peipei Deng, Qinying Gu, Huawu Yin, Guohua Xia, Xiaobin Jia, Huan Yang, and James Tam
quality of bioactive chemical constituents in natural products [ 15 ]. Accordingly, in our previous studies, gallic acid, methyl gallate, and a few non-specific flavonoids such as rutin, kaempferol 3- O - β - d -glucopyranoside, and quercitrin, in Red Toon
Authors:Omar M. Khalaf, Mosad A. Ghareeb, Amal M. Saad, Hassan M. F. Madkour, Ahmed K. El-Ziaty, and Mohamed S. Abdel-Aziz
:9, v/v), followed by PTLC eluted with (ethyl acetate: MeOH; 2.1:2.9; v/v) to give quercetin 3- O -α- l -rhamnopyranosyl-(1→6)-β- d -glucopyranoside (rutin) ( 5 ), while fraction (V) was purified on silica gel sub-column eluted with (EtOAc–MeOH; 17: 3, v
The quality of two samples (Indian and German mother tincture) of
Linn. prepared from marigold flowers have been evaluated on the basis of homeopathic literature. In addition to non-specific standards, two biomarkers, rutin and quercitin, have been used for microfingerprinting analysis of the samples of mother tincture. The results indicated the total absence of rutin in the Indian mother tincture whereas both rutin and quercitin were present in the German mother tincture. On the basis of these results a ratio calculation was performed. The study will be useful to manufacturers of homeopathic mother tinctures.
Annona squamosa, commonly known as custard apple, possesses various medicinal properties such as antimicrobial, insecticidal, cardiotonic, etc. Leaves of A. squamosa are known to contain various types of flavonoids which are responsible for these activities. The aim of this article was to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the determination of rutin and isoquercitrin in the extracts of A. squamosa and in an in-house developed herbal pharmaceutical formulation. Analysis was performed on TLC aluminum plates precoated with silica gel 60 F254 as the stationary phase. Linear ascending development was carried out in a twin-trough glass chamber saturated with a mobile phase consisting of ethyl acetate-formic acid-glacial acetic acid-ethyl methyl ketone-water 50:7:3:30:10 (ν/ν/ν/ν/ν) at room temperature (25 ± 2°C). A CAMAG TLC scanner III was used for the spectrodensitometric scanning at 366 nm. The system was found to give compact bands for rutin and isoquercitrin (RF values of 0.32 ± 0.05 and 0.59 ± 0.03, respectively). The detector response was linear for concentrations ranging between 200 and 1600 ng per band (r2 = 0.9885 ± 0.0041 for rutin and 0.9828 ± 0.0013 for isoquercitrin). The limits of detection and quantitation were 75 and 100 ng per band for rutin, and 40 and 80 ng per band for isoquercitrin, respectively. The recovery study was carried out by the standard addition method and was found to be 96–107%. The present method was found to be simple, specific, sensitive, and accurate and was used successfully to quantitate rutin and isoquercitrin in the leaf extracts of A. squamosa collected from five different locations of India and in a pellet formulation of the extract.
Authors:G.-F. Shi, Z.-J. Wang, G.-Y. Wang, R.-X. Yao, F.-W. Chen, and Z.-R. Shi
High-performance liquid chromatography-mass spectrometry (HPLC-MS) method coupled with radical reaction for screening active ingredients from perennial fujimoto bean whole herb was established. The active ingredients, present in perennial fujimoto bean whole herb, possess scavenging effects towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide, peroxy radical, and hydroxyl radical. The radical scavenging abilities of these active ingredients were evaluated based on the relative peak areas in the HPLC chromatogram. The results indicate that potent antioxidants are present in the anhydrous methanol extract of perennial fujimoto bean whole herb. Based on HPLC-MS analysis, it was found that the scavenging ability can be mostly attributed to the presence of three compounds: cyanidin-3-o-β-d-glucopyranoside, troxerutin, and rutin. The structures were identified based on the MS and nuclear magnetic resonance (NMR) data. Free radical scavenging activity decreased in the following order: troxerutin > rutin > cyanidin-3-o-β-d-glucopyranoside.
Authors:M. Wójciak-Kosior, G. Matysik, and A. Skalska
In this paper we report the possibilities and advantages of HPTLC for investigation of the acid hydrolysis of six flavonoid glycosides: the 3-arabinoside of quercetin (avicularin), the 3-glucoside of quercetin (isoquercitrin), the 3-rhamnoglucoside of quercetin (rutin), the 7-glucoside of apigenin, the 7-rhamnoglucoside of hesperetin (hesperidin) and the 7-rhamnoglucoside of naringenin (naringin). The results obtained are discussed in the terms of the chemical structures of analyzed compounds. Quantitative determinations of flavonoids were performed by use of densitometry.