Authors:Olivera Djuric, Ljiljana Markovic-Denic, Bojan Jovanovic, and Vesna Bumbasirevic
glycopeptide resistance – vancomycin-resistant enterococci; for Enterobacteriaceae : third-generation cephalosporins (ceftriaxone, cefotaxime, or ceftazidime) and carbapenems (imipenem, meropenem or doripenem, and ertapenem) resistance; and for Gram
Authors:Milica Jovanović, Sofie M. van Dorp, Mitra Drakulović, Dubravka Papić, Sladjana Pavić, Snežana Jovanović, Aleksandar Lešić, Miloš Korać, Ivana Milošević, and Ed J. Kuijper
. difficile isolates were sent to the Reference Laboratory at Leiden for PCR ribotyping, detection of toxin genes ( tcdA and tcdB ) genes, as well as binary toxin genes ( ctdA and ctdB ). In vitro susceptibility to metronidazole, vancomycin
The retention behavior of selected macrocyclic antibiotics (erythromycin, troleandomycin, tylosin, vancomycin, rifamycin B, and rifampicin) has been examined on LiChrospher Si 60 F
HPTLC plates as stationary phase. A wide range of mixtures of alcohols and ketones with hexamethyldisiloxane (HMDSO) in proportions from 0 to 100% (
) and with dimethyl sulfoxide (DMSO) in proportions from 0 to 50% (
) were used as mobile phases. Chromatographic retention data and a possible retention mechanism are discussed.
Authors:Zsuzsa Kreizinger, Kinga Mária Sulyok, László Makrai, Zsuzsanna Rónai, László Fodor, Szilárd Jánosi, and Miklós Gyuranecz
The susceptibility of 29 Bacillus anthracis strains, collected in Hungary between 1933 and 2014, was tested to 10 antibiotics with commercially available minimum inhibitory concentration (MIC) test strips. All strains were susceptible to amoxicillin, ciprofloxacin, clindamycin, doxycycline, gentamicin, penicillin, rifampicin, and vancomycin. Intermediate susceptibility to erythromycin and cefotaxime was detected in 17.2% (5/29) and 58.6% (17/29) of the strains, respectively. Correlations were not observed between the isolation date, location, host species, genotype, and antibiotic susceptibility profile of strains.
Authors:Ferenc Rozgonyi, Eszter Ostorházy, Csaba L. Maródi, and et al.
, D. O., Jochimsen, E., Murfitt, K. The impending apocalypse, the emergence of vancomycin resistance in Staphylococcus spp. Infect Control Hosp Epidemiol; 18 , Suppl, P32. Abstract (1997).
The impending apocalypse, the
Authors:Ewa Dworniczek, Justyna Piwowarczyk, Jacek Bania, Beata Kowalska-Krochmal, Ewa Wałecka, Alicja Seniuk, Izabela Dolna, and Grażyna Gościniak
Enterococci, a complex group of facultative pathogens have become increasingly isolated in various hospital settings. They are considerable frequently cultured from traumatic and surgical wounds. We investigated 57 strains of the species E. faecalis, E. faecium and E. casseliflavus isolated from infected wounds. Their ability to produce virulence factors and their sensitivity to antibiotics were evaluated using phenotypic and genotyping methods. In the phenotype studies, significant portion of the isolates produced biofilm (66.7%) and gelatinase (36.8%). Nearly 30% of the strains expressed hemolytic properties. Only a few produced DNAse (15.8%) and lipase (7.0%). The genes esp, gelE, cylA, cylB, cylM and agg were detected in most of the isolates (38.6–87.7%). All the isolated enterococci were susceptible to vancomycin and were characterized by their low resistance to antibiotics, except aminoglycosides (HLR).
Authors:A. Kanghae, J. Monkai, P.D. Eungwanichayapant, P. Niamsup, and E. Chukeatirote
In this study, two bacilli strains, namely TN51 and TN69, previously isolated from Thua Nao, a Thai traditional fermented soybean, were studied in terms of their phenotypic and biochemical properties. Initially, both strains were subjected to morphological determination and a series of biochemical tests. Both were Gram-positive, endosporeforming bacilli. Based on 16S rRNA gene sequence analysis, the identities of strains TN51 and TN69 were confirmed as Bacillus subtilis and B. cereus, respectively. In addition, these two strains were also assessed for their antibiogram profiles. It was found that both strains were susceptible to chloramphenicol, erythromycin, kanamycin, tetracycline, and vancomycin and resistant to ampicillin and intermediately susceptible to bacitracin.
Authors:Boris Habrun, Ivana Račić, Relja Beck, Ana Budimir, Miroslav Benić, Gordan Kompes, Silvio Špičić, and Željko Cvetnić
Methicillin-resistant Staphylococcus aureus (MRSA) have emerged worldwide and have become resistant to a variety of antibiotics. MRSA colonisation in pigs was first reported from the Netherlands in 2005, where pigs were implicated as a source of human MRSA infections (Voss et al., 2005). This paper presents the first report on the presence of MRSA on large pig breeding farms in Croatia, together with the determination of the mecA gene, the results of spa typing and susceptibility to commonly used antimicrobials. Dust samples (7–11 per farm) were collected from eight large pig farms in Croatia. Of the total 68 swabs, the mecA gene was detected in 24 isolates growing on the MRSA agar. All isolates were resistant to oxacillin, tetracycline and streptomycin, and susceptible only to vancomycin, while 92% of the strains were susceptible to ciprofloxacin. Genotyping of the MRSA strains was performed by spa typing, and revealed t011 (n = 17), t034 (n = 5) and t1451 (n = 2). The results presented here predict that MRSA is present on a large number of pig farms in Croatia.
Authors:Osman Tel, Özkan Aslantaş, Oktay Keskin, Ebru Yilmaz, and Cemil Demir
In this study, Staphylococcus aureus strains (n = 110) isolated from seven ewe flocks in Sanliurfa, Turkey were screened for antibiotic resistance and biofilmforming ability as well as for genes associated with antibiotic resistance and biofilm-forming ability. All isolates were found to be susceptible to oxacillin, gentamicin, clindamycin, cefoxitin, tetracycline, vancomycin, amoxicillin-clavulanic acid, ciprofloxacin and sulphamethoxazole-trimethoprim. The percent proportions of strains resistant to penicillin G, ampicillin and erythromycin were 27.2% (n = 30), 25.4% (n = 28) and 6.3% (n = 7), respectively. Regarding the antibiotic resistance genes, 32 (29%) isolates carried the blaZ and 8 (7.2%) the ermC gene. Other resistance genes were not detected in the isolates. All isolates showed biofilm-forming ability on Congo red agar (CRA), while 108 (98.18%) and 101 (91.81%) of them were identified as biofilm producers by the use of standard tube (ST) and microplate (MP) methods, respectively. All isolates carried the icaA and icaD genes but none of them harboured the bap gene. The results demonstrated that S. aureus isolates from gangrenous mastitis were mainly resistant to penicillins (which are susceptible to the staphylococcal beta-lactamase enzyme), and less frequently to erythromycin. Furthermore, all of the S. aureus isolates produced biofilm which was considered a potential virulence factor in the pathogenesis of staphylococcal mastitis.