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Adonina, I. G., Salina, E. A., Pestsova, E. G., Röder, M. S. (2005): Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in

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Kosuge, K., Watanabe, N., Kuboyama, T., Melnik, V.M., Yanchenko, V.I., Rosova, M.A., Goncharov, N.P. 2008. Cytological and microsatellite mapping of mutant genes for spherical grain and compact spikes in durum wheat. Euphytica 159 :289

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Dobrovolskaya, O.B., Arbuzova, V.S., Lohwasser, U., Röder, M.S., Börner, A. 2006. Microsatellite mapping of complementary genes for purple grain colour in bread wheat ( Triticum aestivum L.). Euphytica 150 :355

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Bowers, J. E., Dangl, G. S., Meredith, C. P. (1996) Development and characterization of additional microsatellite DNA markers for grape. Am. J. Enol. Vitic. 50 , 243

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wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers. — Theor. Appl. Genet. 103 : 191–196. Bianchi D. S. Search for evidence

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.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V., Börner, A. 2004. Mapping of 99 new microsatellite-derived loci in rye ( Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. 109 :725

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1472 1479 Somers, D.J., Issac, P., Edwards, K. 2004. A high-density wheat microsatellite consensus map for bread wheat ( Triticum aestivum L.). Theor. Appl. Genet. 109 :1105

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microsatellite screening. Theoretical and Applied Genetics 96 :1110–1120. Law C.N. Genetic analysis of the dwarfing gene Rht8 in wheat. Part II. The distribution and adaptive significance

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Altinkut, A., Gozukirmizi, N. (2003) Search for microsatellite markers associated with water-stress tolerance in wheat through bulked segregant analysis. Mol. Biotechnol. 23, 97

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Bread wheat is the primary bread crop in the majority of countries in the world. The most important product that is manufactured from its grain and flour is yeast bread. In order to obtain an excellent bread, grain with high physical properties is needed for flour and dough. The Russian spring wheat cultivar Saratovskaya 29 is characterized by its exclusively high physical properties of flour and dough. The purpose of this work was to identify the chromosomes carrying the main loci for these traits in Saratovskaya 29 and to map them using recombinant substitution lines genotyped with molecular markers. A set of inter-varietal substitution lines Saratovskaya 29 (Yanetzkis Probat) was used to identify the “critical” chromosomes. The donor of individual chromosomes is a spring cultivar with average dough strength and tenacity. Substitution of 1D and 4D*7A chromosomes in the genetic background of Saratovskaya 29 resulted in a significant decrease in the physical properties of the dough. Such a deterioration in the case of 1D chromosome might be related to the variability of gluten protein composition. With the help of recombinant substitution double haploid lines obtained from a Saratovskaya 29 (Yanetzkis Probat 4D*7A) substitution line the region on the 4D chromosome was revealed in the strong-flour cultivar Saratovskaya 29, with the microsatellite locus Xgwm0165 to be associated with the unique physical properties of flour and dough. The detected locus is not related to the composition gluten proteins. These locus may be recommended to breeders for the selection of strong-flour cultivars. Additionally, a QTL associated with vitreousness of grain was mapped in the short arm of chromosome 7A.

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