Sphagnum based on microsatellitemarkers are reported as of 0.594 for S. cossonii , 0.457 for S. revolvens ( Kophimai et al . 2014 ), 0.174 for S. junghuhnianum , 0.367 for S. palustre and 0.298 for S. imbricatum . Korpelainen et al . (2008
Authors:M.A. Adebayo, A. Menkir, S. Hearne, and A.O. Kolawole
Adebayo , M.A. , Menkir , A. , Malaku , G. , Blay , E. , Gracen , V. , Danquah , E. , Ladejobi , O.
2015 . Diversity assessment of drought tolerant exotic and adapted maize inbred lines with microsatellitemarkers . J. Crop Sci
Authors:A. Kaushik, S. Jain, V. Bhankar, and R. Jain
Availability of markers that distinguish commercially important premium Basmati rice varieties from cheaper Basmati or non-Basmati indica rice varieties is of interest to exporters, commercial suppliers and consumers to ensure the varietal purity of Basmati rice supplies. In this study, a fingerprint database of 27 rice varieties including commercially important traditional Basmati (TB), cross-bred (evolved) Basmati and some non-Basmati indica rice varieties has been prepared using 50 microsatellite markers. A total of 271 electromorphs (alleles) were detected at 50 SSR (simple sequence repeat) loci, 92 of which were observed only in 2–10 of the 16 Basmati rice varieties. Notably, 7–10 of the sixteen Basmati rice varieties had the same Basmati specific electromorphs at RM562, RM551 and RM547 loci. Alarge number of unique (22) and null (16) electromorphs were noticed in Basmati rice varieties. A combination of four SSR markers (RM224, RM248, RM547 and RM594) can be used to differentiate all the 27 rice varieties. Specific SSR markers have been identified for the authentication of Premium Basmati rice cultivars such as Taraori Basmati (RM547, RM594 and RM511), Basmati 370 (RM252, RM426 and RM527), Karnal Local (RM248, RM423 and RM488) and Pusa 1121 (RM252, RM400 and RM410), and for the detection of adulterant like Sharbati (RM215, RM423 and RM259). SSR database reported here, shall broaden the list of SSR markers already recommended for varietal identification and detection of adulteration in Basmati rice supplies.
Authors:M. Molnár-Láng, G Linc, E. D. Nagy, and et al.
New wheat × barley, wheat × Aegilops biuncialis and wheat × rye hybrids were produced with the aim of alien gene transfer from these species into wheat. Amphiploids were produced with the help of colchicine treatment from the last two combinations. The new wheat × barley hybrids were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Wheat-barley chromosome pairing was detected using genomic in situ hybridization (GISH) in two combinations (Mv9 kr1 × Igri, Asakazekomugi × Manas). In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants. Five wheat-barley translocations were produced in a wheat background and characterized through the combination of cytogenetic and molecular genetic approaches (GISH, FISH and SSR markers). The following translocations were identified: 2DS.2DL-1HS, 3HS.3BL, 6BS.6BL-4HL, 4D-5HS and 7DL.7DS-5HS. Physical mapping of the SSR markers on chromosomes 1H and 5H was carried out using the intragenomic and interspecific translocation breakpoints and the centromere as physical landmarks. Disomic wheat-Aegilops biuncialis additions were produced after backcrossing the wheat-Ae. biuncialis amphiploids. Fluorescence in situ hybridization (FISH) was carried out using two repetitive DNA clones (pSc119.2 and pAs1) on Ae. biuncialis and its two diploid progenitor species to detect chromosome polymorphism. The 7M and 3M disomic chromosome additions were selected and five more lines still need to be characterized. The octoploid triticale (Mv9 kr1 × Lovászpatonai) produced in Martonvásár was crossed with a 1RS.1BL wheat cultivar Matador. GISH analysis detected pairing between the 1RS arm of the translocation chromosome and that of Lovászpatonai rye in 32 % of the pollen mother cells, making it possible to select recombinants from this combination. The new recombinants between the 1RS of Petkus and the 1RS of Lovászpatonai rye cultivars are being analysed with the help of microsatellite markers.
Authors:J. Anerao, G. Sharangi, V. Jha, V. Pardhi, S. Chavan, N. Desai, and K. Mangaonkar
, W ., Morgante , M ., Andre , C ., Hanafey , M ., Vogel , J ., Tingey , S . and Rafalski , A . ( 1996 ): The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis . – Mol. Breed . 2 : 225 – 238 . 10