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Nicholson, V. M. and Prescott, J. F. (1997): Restriction enzyme analysis of the virulence plasmids of VapA-positive Rhodococcus equi strains isolated from humans and horses. J. Clin. Mi-crobiol. 35 , 738-740. Restriction enzyme

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(in Hungarian) . Ph.D. Thesis, Hungarian Academy of Sciences, Budapest. Benkő , M. , Bartha , A. and Wadell , G. ( 1988 ): DNA restriction enzyme analysis of bovine

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Acta Veterinaria Hungarica
Authors: S. Bodó, B. Baranyai, Elen Gócza, J. Dohy, and Merja Markkula

digestion with two restriction enzymes . Anim. Genet. 27 , 207 – 209 . Zsolnai , A. and Fésüs , L. ( 1997 ): Enhancement of PCR-RFLP typing of bovine leukocyte adhesion deficiency

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. Meulemans , G. , Boschmans , M. , van den Berg , T. P. and Decaesstecker , M. ( 2001 ): Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses . Avian Pathol. 30 , 655 – 660

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Acta Microbiologica et Immunologica Hungarica
Authors: M. Peterka, Katarina Tepšič, T. Accetto, R. Kostanjšek, Andreja Ramšak, L. Lipoglavšek, and G. Avguštin

Wood, J., Scott, K. P., Avgustin, G., Newbold, C. J., Flint, H. J.: Estimation of the relative abundance of different Bacteroides and Prevotella ribotypes in gut samples by restriction enzyme profiling of PCR-amplified 16S rRNA gene sequences. Appl

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was studied by pulsed-field gel electrophoresis (PFGE). The cell agarose suspension was prepared and lysed according to Morrison et al. (1999) . The chromosomal DNA of these strains was digested by SmaI restriction enzyme (Sigma, USA) and was run in a

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peptides were utilized for reverse translation and codon optimization. Finally, some additional sequences were added (Figure  1B and 1C ). The final sequences were synthesized (Generay biotechnology, China), digested by BamHI and XhoI restriction enzymes

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–horseradish peroxidase coupled antibody for detection. RFLP analysis of phage DNAs Phage DNAs were cut with Hin fI restriction enzyme and run through an EtBr-treated gel and were visualized and photographed

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Acta Microbiologica et Immunologica Hungarica
Authors: Sára Szuróczki, Zsuzsa Kéki, Szandra Káli, Anett Lippai, Károly Márialigeti, and Erika Tóth

restriction analysis (ARDRA) to group the 170 bacterial strains The amplified 16S rRNA gene fragments were digested using Bsu RI (GG CC) and Msp 1 (C CGG) restriction enzymes (10 U μL −1 , Fermentas), as described by Massol-Deya et al. [16] . The

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]. The complete bacterial genome was embedded in agarose plugs and lysed in several steps to purify DNA. For the digestion, Sma I restriction enzyme was used (3 h, 25 °C). The digested samples were run in a 1% agarose gel along with N0340S Lambda Ladder

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