Clostridium perfringens is a widely distributed foodborne pathogen. Its ability to survive cold encounters could contribute to its persistence in foods and the potential to cause disease. In this work five cold-shock proteins (101, 82, 70, 45 and 10 kDa) were induced by cold-shocking C. perfringens FD-1041 from 43 °C to 28 °C, as revealed by labeling with L-[35S]methionine and cysteine followed by gel electrophoresis. Cold shock also increased the cold tolerance of the cells at least fifteen fold. The acquired tolerance was maintained for 2 h after the cold treatment. This ability of C. perfringens could improve the survival in foods and present a significant hazard.
The paper presents results of the HPLC determination of sulphachloropyrazine residues (active component of the drug „Esb3 30%”) in muscle tissue and liver of broiler chickens inoculated with laboratory-grown coccidium in the course and after treatment with this sulphonamide.Extraction of sulphachloropyrazine from samples of broiler muscle tissue and liver was carried out with a mixture of solvents dichloromethane-methanol-acetic acid (90:5:5, v/v/v), followed by extract purification by chromatographic separation on a XAD-2 column and elution of sulphachloropyrazine residues with dichloromethane. The HPLC determination of sulphachloropyrazine residues was accomplished on a Bio Sil C-8 HL 5 ?m column with a mobile phase consisting of 60% aqueous solution of acetonitrile and NH3 (pH=9.5), using a UV detector at 254 nm.The method developed allows quantitative determination of the residues of the anticoccidial agent in broiler tissue samples with a detection limit of 0.02 ?g g–1. Recovery of the method for this type of samples with a complex matrix was satisfactory, the results ranging from 79.2(0.6 to 86.7(0.2% for muscle tissue and from 81.7(0.8 to 87.3(0.7% for liver.
Authors:Z. Lisiewska, W. Kmiecik, P. Gębczyński, and L. Sobczyńska
Cruz-Garcia, C., Gonzalez-Castro, M.J., Oruna-Concha, M.J., Lopez-Hernandez, J., Simal-Lozano, J.A. & Simal-Dandara, J. (1997): The effects of various culinary treatments on the pigment content of green beans ( Phaseolus vulgaris L.). Fd Res. int
Authors:É. Andrássy, J. Farkas, Zs. Seregély, I. Dalmadi, E. Tuboly, and V. Lebovics
Experiments were performed to study changes caused by irradiation or high hydrostatic pressure pasteurization of liquid egg white by differential scanning calorimetry, spectrofluorimetry, electronic nose measurements and NIR-spectrometry. The non-thermal pasteurization treatments were also assessed in relation to loss of carotenoid content, and lipid- and cholesterol oxidation of liquid egg yolk. Unlike radiation pasteurization, high pressure processing caused protein denaturation in egg white, which manifested in changes of its DSC-thermogram and intrinsic tryptophan fluorescence. Electronic nose testing showed changes of the head-space volatile composition of egg albumen, particularly as a function of radiation treatment. Both treatments caused changes in the NIR-spectrometric “fingerprint” of the liquid egg white. Various chemometric analyses of the results of the latter instrumental methods, particularly statistical techniques developed by the group of one of the co-authors of this article, demonstrated the potential for detection and characterization of the applied non-thermal processing techniques on liquid egg white. Irradiation induced more carotenoid degradation and lipid oxidation in liquid egg yolk than pressure processing.
Authors:Kalman Buzas, Peter Budai, and Adrienne Clement
One and a half year long field sampling was established in order to evaluate the contamination of storm water runoff. The event means (EMC) total petroleum hydrocarbons (TPH) concentration of the runoff was a function of the actual traffic intensity and the rainfall depth. It was concluded that this TPH, of which dominant component is the engine-oil (characteristically C28) does not form an ‘oil-in-water’ type emulsion in the condition of normal motorway-operation, but is interlocked to asphalt, rubber and soot particles of maximum some ten microns size. This condition influences the possibility, way and extent of separation alike. The separation equipments and their sizing applied so far are not suitable to achieve efficient surface water protection. Practical prevention method of accidental type environment pollution was also suggested. On the basis of the international literature evaluation, technical solutions capable to decrease the runoff pollution were summarized. Among these, useful calculation method was developed for designers to determine the sufficient storage capacity (water quality volume) of reservoirs applied for water quality protection. Monitoring technique of runoffs, which are characterized time dependent, variable pollutant concentrations by their nature, was proposed. It was stated that the administrative regulation should aim at the EMC instead of current concentration. Beside TPH information was gained on polycyclic aromatic hydrocarbons (PAHs), suspended solids, heavy metals, pH, and nutrient (N, P) pollution of the runoff, which are valuable for the grounding of further research results.
Authors:Szilvia Ormándi, Ildikó Cora, Zsolt Dallos, Ferenc Kristály, and István Dódony
Zeolites have complex alumosilicate frameworks. These frameworks contain large channels and interconnected cages. We use zeolites in gas and petroleum industry, water softening, sewage treatment
Authors:Alessandro Benedetti, Luis Miguel Sender, Ignacio Escapa, Rubén Cúneo, and José Bienvenido Diez
treatment or sample preparation was required. The fossil samples were mounted on a SEM stub and then Au-coated to make them conductive.
The use of a FEI Helios 600 Nanolab FIB, equipped with a field emission gun (FEG) and an Inca X-ray analysis
Authors:Monika Furko, Zsolt Fogarassy, Katalin Balázsi, and Csaba Balázsi
chemical oxidization treatment (GO1), the graphene have become even more exfoliated, and individual GO sheets can be observed with wrinkled edges. After thermal oxidization, the morphology of GO particles has changed, and smaller-sized platelets (100–600 nm
castings are normally (but not totally) limited to non-structural components that do not require such a heat treatment or welding. Porosity causes more rejected castings than any other factor.
In cold-chamber die casting of aluminum alloys, air and
Authors:H. Al-Maliki, Z. Károly, Sz. Klébert, and G. Kalácska
, Williams R. L. , Pond R. C. ( 2001 ), Plasma modification of PTFE surfaces. Part I: Surfaces immediately following plasma treatment . Surface and Interface Analysis , 31 , 385 – 396 . DOI: 10.1002/sia